Cell‐lined, Nonwoven Microfiber Scaffolds as a Blood Interface*

Burkel, William E.; Kahn, Raymond H.

doi:10.1111/j.1749-6632.1977.tb41787.x

Cited by 15 publications

(5 citation statements)

References 32 publications

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“…At the same time most earlier investigations were undertaken to produce pseudointimas from cells or tissue fragments in vitro on special surfaces such as diaphragms of artificial hearts, nonporous microfiber-lined prostheses, etc. [2]. Many of these studies had limited relevance to development of vascular conduits for use in peripheral vascular surgery.…”

Section: Discussionmentioning

confidence: 99%

“…During the past 20 years numerous investigators have endeavored to improve the performance of vascular prostheses by seeding cells or tissue fragments into them [2]. Recently, Herring and his colleagues demonstrated that tissue stripped from veins with steel-wool pledgets and subsequently seeded into vascular prostheses during graft preclotting enhanced development of an endothelial lining [ll].…”

mentioning

confidence: 99%

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Sequential studies of healing in endothelial seeded vascular prostheses: Histologic and ultrastructure characteristics of graft incorporation

Burkel

Vinter

Ford

et al. 1981

Journal of Surgical Research

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Chronological events leading to incorporation of endothelial cell seeded prosthetic vascular grafts were documented in this investigation. Forty-one adult dogs underwent thoracoabdominal bypass using double-velour Dacron grafts. Experimental grafts were preclotted with blood containing enzymatically derived endothelium immediately after derivation, or after 14 days of cultivation. Control grafts were preclotted without addition of endothelial cells. Grafts were studied grossly as well as by light, scanning electron, transmission electron, and fluorescence microscopy, 1 to 28 days postimplantation. Control graft healing proceeded from pannus and perigraft ingrowth. Experimental grafts healed from seeded cells as well. Platelets covered all grafts by Days 1 and 2. Thrombus accumulations on control grafts, first evident on Day 4, became maximal by Day 14. Seeded grafts appeared relatively thrombus free with patches of endothelial cells noted by 4 days. These cells were initially separated by gaps, often containing leukocytes. Endothehum became densely packed with cellular migration and proliferation. Subendothelial tissues were composed of fibrin and smooth muscle. Control and experimental grafts were approximately 10 and 80% endothelialized, respectively, by Day 28. Smooth muscle dominated subintimal tissue in experimental grafts. These cells initially appeared fibroblastoid. Endothelial seeding enhances both pseudointimal development and rapid graft incorporation.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Sequential studies of healing in endothelial seeded vascular prostheses: Histologic and ultrastructure characteristics of graft incorporation

Burkel

Vinter

Ford

et al. 1981

Journal of Surgical Research

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“…Parylene C, a material with well-documented biocompatibility and FDA-approval, is used as a flexible and robust framework and nanodiamond packaging agent for microfilm fabrication. , Parylene surfaces have been utilized in several medical applications because of their highly conformal nature, biostability, and inertness under physiological conditions with no known adverse biological degradation events. ,− …”

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confidence: 99%

Nanodiamond-Embedded Microfilm Devices for Localized Chemotherapeutic Elution

et al. 2008

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Nanodiamonds (NDs) of 2-8 nm diameters physically bound with the chemotherapeutic agent doxorubicin hydrochloride (DOX) were embedded within a parylene C polymer microfilm through a facile and scalable process. The microfilm architecture consists of DOX-ND conjugates sandwiched between a base and thin variable layer of parylene C which allows for modulation of release. Successive layers of parylene and the DOX-ND conjugates were characterized through atomic force microscopy (AFM) images and drug release assays. Elution rates were tested separately over a period of 8 days and up to one month in order to illustrate the release characteristics of the microfilms. The microfilms displayed the stable and continuous slow-release of drug for at least one month due to the powerful sequestration abilities of the DOX-ND complex and the release-modulating nature of the thin parylene layer. Since the fabrication process is devoid of any destructive steps, the DOX-ND conjugates are unaffected and unaltered. A DNA fragmentation assay was performed to illustrate this retained activity of DOX under biological conditions. Specifically, in this work we have conferred the ability to tangibly manipulate the NDs in a polymer-packaged microfilm format for directed placement over diseased areas. By harnessing the innate ND benefits in a biostable patch platform, extended targeted and controlled release, possibly relevant toward conditions such as cancer, viral infection, and inflammation, where complementary alternatives to systemic drug release enabled by the microfilm devices, can allow for enhanced treatment efficacy.

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“…On the other hand, cells will not attach themselves and grow on surfaces that are hostile to them. For example, Figure 14 is a SEM picture of a section of a poly-(tetramethylene terephthalate) microfiber web attached to a polyurethane tube, seeded with WI-38 cells and cultured for seven days [46]. Apparently owing to some toxic leachable components or residual solvent in the polyurethane, the pseudointima became necrotic, as is visible in the central portion of the photograph.…”

Section: Substrate Effects On Tissue-cultured Vascular Prosthesesmentioning

confidence: 99%

“…Contamination either in the microfibers or in the polyurethane can also kill the cells, as shown in Figures 15 and 16. Physical irregularities within the poly(tetramethy1ene terephthalate) microfiber web, such as shown in Figures 17-19, do not provide a good substrate on which the cells can grow [46]. On the other hand, as shown in Figure 20, under optimum conditions with carefully prepared substrates, cells seeded onto microfiber substrates in a perfusion apparatus can form a good live coverage [46].…”

Section: Substrate Effects On Tissue-cultured Vascular Prosthesesmentioning

confidence: 99%

Physicochemical aspects of the blood compatibility of polymeric surfaces

Bruck

1979

J. polym. sci., C Polym. symp.

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SYNOPSISThe hypothesis is advanced that electrical conduction and semiconduction properties of some natural biopolymers and synthetic polymers may have a relationship to blood compatibility. Newly developed impervious, thin carbon coatings perform best biologically when deposited on smooth polymeric and nonpolymeric surfaces. The biological performance of grafted polyacrylamide hydrogels is influenced by both substrate and hydrodynamic effects which may be more dominant in blood compatibility than surface energy forces. Although the latter are undoubtedly involved in bioadhesion, their contributions do not correlate well with the overall biological performance of synthetic materials. Species-related hematological differences of experimental animals, particularly calves, dogs, and primates, significantly influence the biological performance of materials which must be considered in the human application of medical devices in which biomaterials are in contact with blood.

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Cell‐lined, Nonwoven Microfiber Scaffolds as a Blood Interface*

Cited by 15 publications

References 32 publications

Sequential studies of healing in endothelial seeded vascular prostheses: Histologic and ultrastructure characteristics of graft incorporation

Sequential studies of healing in endothelial seeded vascular prostheses: Histologic and ultrastructure characteristics of graft incorporation

Nanodiamond-Embedded Microfilm Devices for Localized Chemotherapeutic Elution

Physicochemical aspects of the blood compatibility of polymeric surfaces

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