Cell lines containing varicella-zoster virus open reading frame 62 and expressing the "IE" 175 protein complement ICP4 mutants of herpes simplex virus type 1

Felser, James M.; Kinchington, Paul R.; Inchauspé, Geneviève; Straus, Stephen E.; Ostrove, Jeffrey M.

doi:10.1128/jvi.62.6.2076-2082.1988

Cited by 75 publications

(45 citation statements)

References 38 publications

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“…ICP4 is indispensable to the life cycle of the virus, is involved in negative regulation of IE genes, and is required throughout the viral replicative cycle for the expression of early and late genes (31). We previously showed that ORF62 protein is the functional homolog of HSV-1 ICP4 by demonstrating that ORF62-expressing cell lines can complement ICP4 mutants (15). This was confirmed when an HSV-1 recombinant which contains VZV ORF62 in place of the coding sequences for ICP4 was shown to grow in cell culture (11).…”

mentioning

confidence: 79%

“…African green monkey kidney cells (Vero; American Type Culture Collection, Rockville, Md.) were propagated as previously described (15) (20), were grown in Eagle's minimal essential medium supplemented with 10% heat-inactivated fetal bovine serum, 2 mM glutamine, penicillin G (100 U/ml), and streptomycin (100 pug/ml). FI-14 cells (VZV ORF62-expressing Vero cells [15]) and 3-3 cells (HSV-1 ICP27-expressing Vero cells [24]), provided by Priscilla A. Schaffer, were propagated as previously described.…”

Section: Methodsmentioning

confidence: 99%

“…were propagated as previously described (15) (20), were grown in Eagle's minimal essential medium supplemented with 10% heat-inactivated fetal bovine serum, 2 mM glutamine, penicillin G (100 U/ml), and streptomycin (100 pug/ml). FI-14 cells (VZV ORF62-expressing Vero cells [15]) and 3-3 cells (HSV-1 ICP27-expressing Vero cells [24]), provided by Priscilla A. Schaffer, were propagated as previously described. HSV-1 7134 (an ICPO null mutant [3]), HSV-1 Sdll.2 (an ICP27 deletion mutant [32]), and d120 (an HSV-1 ICP4 deletion mutant [9]) were provided by Priscilla A. Schaffer.…”

Section: Methodsmentioning

confidence: 99%

“…Vero and MeWo cells were cotransfected with pSV2neo and pMTPORF61R or pMTPORF61I. G418-resistant cell colonies were selected and subcloned as described previously (15) with the following modifications. About 6 x 105 Vero or 1.2 x 106 MeWo cells were plated the day before transfection in 60-mm-diameter dishes.…”

Section: Methodsmentioning

confidence: 99%

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Varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICP0

Moriuchi

Smith

et al. 1992

J Virol

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The varicella-zoster virus (VZV) open reading frame 61 (ORF61) protein is thought to be the homolog of herpes simplex virus type 1 (HSV-1) ICPO, based on gene location and limited amino acid homology. However, HSV-1 ICPO trans activates HSV-1 genes, while VZV ORF61 protein trans represses the function of VZV trans activators on VZV promoters in transient expression assays. To investigate the functional relatedness of HSV-1 ICPO and VZV ORF61 protein, we established Vero and MeWo cell lines which stably express VZV ORF61 under the control of a metallothionein promoter and performed complementation studies with an HSV-1 ICPO deletion mutant (7134). Mutant 7134 is impaired for plaque formation and replication at a low multiplicity of infection in cell culture, but these defects were complemented by up to 200-fold in Vero cell lines expressing VZV ORF61. Likewise, the efficiency of plaque formation was improved by up to 100-fold in MeWo cell lines expressing VZV ORF61. A cell line expressing another VZV immediate-early gene product (0RF62) was unable to complement mutant 7134. HSV-1 mutants which are deleted for other HSV-1 immediate-early gene products (ICP4, ICP27) were unable to grow in VZV ORF61-expressing cell lines. These results indicate that, despite marked differences in their sequences and in effects on their cognate promoters in transient expression assays, VZV ORF61 protein is the functional homolog of HSV-1 ICPO.

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mentioning

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICP0

Moriuchi

Smith

et al. 1992

J Virol

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“…DNA sequence analysis (Davison and Scott, 1986) and recent reports (Felser et al, 1988) have identified VZV genes that arc similar to HSV immediate early (IE) genes. VZV gene IE 62 encodes a protein of 175 Kd, which is the functional analog of HSV-1 ICP4, whereas VZV gene 63 displays sequence homology to HSV-I ICP22 (30 Kd).…”

Section: Introductionmentioning

confidence: 99%

Acute and persistent varicella‐zoster virus infection of human and murine neuroblastoma cell lines

Bourdon-Wouters¹,

Merville-Louis²,

Sadzot-Delvaux³

et al. 1990

J of Neuroscience Research

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Human and murine neuroblastoma cell lines were infected in vitro with varicella-zoster virus (VZV). Infected human neuroblastoma cells (IMR-32) supported the synthesis of abundant viral antigens as detected by indirect immunoperoxidase labeling using human serum rich in anti-VZV antibodies and did not survive the infection. In situ hybridization (ISH) with VZV-cloned probes revealed a strong hybridization signal in these infected cells. During cultivation, the virus was released in the culture medium, and viral polypeptides were revealed by Western blotting of infected cells, using either a monoclonal anti-gpI antibody or a rabbit antiserum. All these findings indicate that IMR-32 cells support a productive and lytic infection by VZV, whether infected by cell-free virus or by cocultivation with infected cells. Murine neuroblastoma cells (neuro-2A) survived VZV infection and did not produce any infectious virus. No VZV-specific proteins were detected in infected cells either by immunolabeling or by Western blotting. However, viral nucleic acids could be detected by ISH, indicating that mouse neuroblastoma cells displayed a nonproductive, nonlytic infection. Infected neuro-2A cells have been examined by ISH using probes corresponding to immediate early (IE) genes 4, 62, and 63 and late (L) gene 31 encoding gpII. A strong hybridization signal was detected when infected cells were probed with a fragment containing the IE genes 62 and 63. Lower levels of hybridization were detected with the other probes, corresponding to IE or L genes. These systems allow comparative molecular analysis of persistent and acute infection of nerve cells by VZV.

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Enhancement of varicella-zoster virus infection in cell lines expressing ORF4- or ORF62-encoded proteins

et al. 1996

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Varicella-Zoster virus (VZV) open reading frames 4 (ORF4) and 62 (ORF62) encode putative immediate early proteins (ORF4p and ORF62p, respectively) which are strong transactivators of other VZV genes and are involved in the very early stages of viral infection. ORF4p and ORF62p transactivate immediate-early and early gene promoters but have little or no effect on late gene promoters. To investigate the effect of ORF4p or ORF62p overexpression on the viral replication cycle, we constructed Vero cell lines expressing those genes under the control of the human cytomegalovirus major immediate-early promoter. VZV OKA infection of these stably transformed cell lines was followed-up using VZV glycoprotein E (gE) antigen quantification and virus titration. Upon serial passaging of infection in these cell lines expressing functionally active ORF4p or ORF62p, a 5- to 10-fold increase in viral gE antigen production was observed. Viral titers also demonstrated a 2- to 5-fold increase in viral production in these transformed cell lines. These results emphasize the role that both ORF4p and ORF62p play in enhancing the VZV replicative cycle.

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Cell lines containing varicella-zoster virus open reading frame 62 and expressing the "IE" 175 protein complement ICP4 mutants of herpes simplex virus type 1

Cited by 75 publications

References 38 publications

Varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICP0

Varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 ICP0

Acute and persistent varicella‐zoster virus infection of human and murine neuroblastoma cell lines

Enhancement of varicella-zoster virus infection in cell lines expressing ORF4- or ORF62-encoded proteins

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