Cell lysis methods for high‐throughput screening or miniaturized assays

Lin, Zhanglin; Cai, Zhen

doi:10.1002/biot.200800222

Cited by 23 publications

(12 citation statements)

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“…For example, cells can be lysed and the intracellular products can directly be post processed (PCR or DNA isolation for diagnostics) inside the same device [ 47 , 48 ]. Although there have been a number of reviews on cell lysis in the past 10 years [ 7 , 8 , 49 ], some of the recent developments in the field have not been reviewed. This review will focus on the recent developments from 2014 onwards and will briefly cover the developments from before, which have been extensively surveyed.…”

Section: Microfabricated Platforms For Cell Lysismentioning

confidence: 99%

A Review on Macroscale and Microscale Cell Lysis Methods

2017

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The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit operation in biomolecular analysis. This paper presents a critical evaluation of the various methods that are available both in the macro and micro scale for cell lysis. Various types of cells, the structure of their membranes are discussed initially. Then, various methods that are currently used to lyse cells in the macroscale are discussed and compared. Subsequently, popular methods for micro scale cell lysis and different microfluidic devices used are detailed with their advantages and disadvantages. Finally, a comparison of different techniques used in microfluidics platform has been presented which will be helpful to select method for a particular application.

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Section: Microfabricated Platforms For Cell Lysismentioning

confidence: 99%

A Review on Macroscale and Microscale Cell Lysis Methods

2017

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“…Mechanical techniques, such as cell ultrasonication, usually result in protein denaturation caused by the heat produced during the process and some of them are also unfeasible on industrial scale, whereas non-mechanical techniques, such as chemical membrane degradation with detergents or enzymes, involve the purchase of expensive reagents [8]. Other recently proposed mechanical, chemical, physical and enzymatic treatments to disrupt the cell membrane, especially focused on high throughput screening, are reviewed in [10]. The engineering of a lysis system that is triggered by a user-defined signal can avoid the use of common cell disruption techniques for the recovery of intracellularly expressed proteins.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

et al. 2011

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BackgroundBacterial cell lysis is a widely studied mechanism that can be achieved through the intracellular expression of phage native lytic proteins. This mechanism can be exploited for programmed cell death and for gentle cell disruption to release recombinant proteins when in vivo secretion is not feasible. Several genetic parts for cell lysis have been developed and their quantitative characterization is an essential step to enable the engineering of synthetic lytic systems with predictable behavior.ResultsHere, a BioBrick™ lysis device present in the Registry of Standard Biological Parts has been quantitatively characterized. Its activity has been measured in E. coli by assembling the device under the control of a well characterized N-3-oxohexanoyl-L-homoserine lactone (HSL) -inducible promoter and the transfer function, lysis dynamics, protein release capability and genotypic and phenotypic stability of the device have been evaluated. Finally, its modularity was tested by assembling the device to a different inducible promoter, which can be triggered by heat induction.ConclusionsThe studied device is suitable for recombinant protein release as 96% of the total amount of the intracellular proteins was successfully released into the medium. Furthermore, it has been shown that the device can be assembled to different input devices to trigger cell lysis in response to a user-defined signal. For this reason, this lysis device can be a useful tool for the rational design and construction of complex synthetic biological systems composed by biological parts with known and well characterized function. Conversely, the onset of mutants makes this device unsuitable for the programmed cell death of a bacterial population.

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“…For example, the freeze thaw technique uses repeated freezing and thawing of cells to form sharp ice crystals within the cells to disrupt the sample. 20 Osmotic shock lysis technique is dependent on the changes of hydrostatic pressure created by a change in concentration of solutes between a semipermeable cell membrane. The osmotic gradient forces water to flow through the cell membrane, and result in breaking the cells apart.…”

Section: Non-mechanical Methods Of Cell Disruptionmentioning

confidence: 99%