Cell manipulation in autologous chondrocyte implantation: from research to cleanroom

Roseti, Livia; Serra, Marta; Tigani, Domenico; Brognara, Irene; Lopriore, Annamaria; Bassi, A.; Fornasari, Pier Maria

doi:10.1007/s12306-007-0024-1

Cited by 15 publications

(11 citation statements)

References 17 publications

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“…ASCs have come to be regarded as the ideal stem cell for regenerative clinical applications since their yield upon isolation is so high that they do not require extensive manipulation before delivery. Thus, there is no need for compliance with “cell manufacturing” in accordance with current Good Manufacturing Practice Guidelines [42]. Restrictions are not applied in the case of non substantial manipulation [Regulation (EC) No 1394/2007 of the European Parliament and of the Council].…”

Section: Discussionmentioning

confidence: 99%

Adipose-derived stem cells: Comparison between two methods of isolation for clinical applications

Raposio

Simonacci

Perrotta

2017

Annals of Medicine &Amp; Surgery

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BackgroundAdipose-derived stem cells are recognized as being an effective mesenchymal stem cell population with enormous potential in different fields of regenerative medicine and stem cell therapy. Although there is unanimous agreement on the harvesting procedure for adipose tissue, there are various protocols for adipose-derived stem cell isolation. The aim of this study was compare two methods of adipose-derived stem cells (ASCs) isolation, one based on a mechanical + enzymatic (ME) procedure and the other one exclusively mechanical (MC), in order to determine which one was superior to the other in accordance with current European and US legislation.MethodsWe reported step by step the two different methods ASCs isolation by comparing them. The ME procedure included the use of a centrifuge, an incubator and collagenase digestion solution (Collagenase NB 6 GMP Grade 17458; Serva GmbH, Heidelberg, Germany). The MC procedure was performed by vibrating shaker and centrifuge, both placed in a laminar airflow bench.ResultsWith the ME procedure, a mean of 9.06 × 105 ASCs (range, 8.4 to 9.72 × 105; SD ± 6.6 × 105) was collected, corresponding to 25.9% of the total number of harvested cells. With the MC procedure, a mean of 5 × 105 ASCs (range: 4.0 to 6.0 × 105; SD, ±1 × 105) was collected, corresponding to 5% of the total number of harvested cells.ConclusionBased on data collected, from the same amount of lipoaspirate the ME procedure allowed to isolate a greater number of ASCs (25,9%) compared to the MC one (5%).

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Section: Discussionmentioning

confidence: 99%

Adipose-derived stem cells: Comparison between two methods of isolation for clinical applications

Raposio

Simonacci

Perrotta

2017

Annals of Medicine &Amp; Surgery

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“…Thus, there is no need for compliance with “cell manufacturing” in accordance with current Good Manufacturing Practice Guidelines [80]. These restrictions do not apply in the case of minimal manipulation [Regulation (EC) No 1394/2007 of the European Parliament and of the Council].…”

Section: Adipose-derived Stem Cells (Ascs)mentioning

confidence: 99%

The biological and clinical basis for the use of adipose-derived stem cells in the field of wound healing

Bertozzi

Simonacci

Grieco

et al. 2017

Annals of Medicine &Amp; Surgery

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Worldwide, hard-to-heal lower limb wounds are estimated to affect 1.5–3% of the adult population with a treatment-related annual cost of $10 billion. Thus, chronic skin ulcers of the lower limb are a matter of economic and public concern. Over the years, multiple medical and surgical approaches have been proposed but they are still inadequate, and no effective therapy yet exists. Regenerative medicine and stem cell-based therapies hold great promise for wound healing. Recently, many plastic surgeons have studied the potential clinical application of adipose-derived stem cells (ASCs), which are a readily available adult stem cell population that can undergo multilineage differentiation and secrete growth factors that can enhance wound-healing processes by promoting angiogenesis, and hence increase local blood supply. ASCs have been widely studied in vitro and in vivo in animal models. However, there are few randomized clinical trials on humans, and these are still ongoing or recruiting patients. Moreover, there is no consensus on a common isolation protocol that is clinically feasible and which would ensure reproducible results. The authors aim to provide readers with an overview of the biological properties of ASCs as well as their clinical application, to help better understanding of present and future strategies for the treatment of hard-to-heal wounds by means of stem cell-based therapies.

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“…This review presents an overview of the knowledge and clinical applications of adult MSCs, highlighting the role Lipogems® technology has played in regenerative medicine so far. The urgent need to find new therapies for chronic immunologic and degenerative diseases prompted many investigators to search for products containing progenitor cells while avoiding the problems and restrictions related to enzymatic manipulation and cell expansion in accordance with good manufacturing practice (GMP) rules [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Adipose Tissue and Mesenchymal Stem Cells: State of the Art and Lipogems® Technology Development

Tremolada¹,

Colombo²,

Ventura³

2016

Curr Stem Cell Rep

197

164

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In the past few years, interest in adipose tissue as an ideal source of mesenchymal stem cells (MSCs) has increased. These cells are multipotent and may differentiate in vitro into several cellular lineages, such as adipocytes, chondrocytes, osteoblasts, and myoblasts. In addition, they secrete many bioactive molecules and thus are considered “mini-drugstores.” MSCs are being used increasingly for many clinical applications, such as orthopedic, plastic, and reconstructive surgery. Adipose-derived MSCs are routinely obtained enzymatically from fat lipoaspirate as SVF and/or may undergo prolonged ex vivo expansion, with significant senescence and a decrease in multipotency, leading to unsatisfactory clinical results. Moreover, these techniques are hampered by complex regulatory issues. Therefore, an innovative technique (Lipogems®; Lipogems International SpA, Milan, Italy) was developed to obtain microfragmented adipose tissue with an intact stromal vascular niche and MSCs with a high regenerative capacity. The Lipogems® technology, patented in 2010 and clinically available since 2013, is an easy-to-use system designed to harvest, process, and inject refined fat tissue and is characterized by optimal handling ability and a great regenerative potential based on adipose-derived MSCs. In this novel technology, the adipose tissue is washed, emulsified, and rinsed and adipose cluster dimensions gradually are reduced to about 0.3 to 0.8 mm. In the resulting Lipogems® product, pericytes are retained within an intact stromal vascular niche and are ready to interact with the recipient tissue after transplantation, thereby becoming MSCs and starting the regenerative process. Lipogems® has been used in more than 7000 patients worldwide in aesthetic medicine and surgery, as well as in orthopedic and general surgery, with remarkable and promising results and seemingly no drawbacks. Now, several clinical trials are under way to support the initial encouraging outcomes. Lipogems® technology is emerging as a valid intraoperative system to obtain an optimal final product that may be used immediately for regenerative purposes.

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Cell manipulation in autologous chondrocyte implantation: from research to cleanroom

Cited by 15 publications

References 17 publications

Adipose-derived stem cells: Comparison between two methods of isolation for clinical applications

Adipose-derived stem cells: Comparison between two methods of isolation for clinical applications

The biological and clinical basis for the use of adipose-derived stem cells in the field of wound healing

Adipose Tissue and Mesenchymal Stem Cells: State of the Art and Lipogems® Technology Development

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