Cell mechanics can be robustly derived from AFM indentation data using the brush model: error analysis

Makarova, Nadezda; Sokolov, Igor

doi:10.1039/d2nr00041e

Cited by 8 publications

(17 citation statements)

References 54 publications

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“…57 Thus, it was interesting to verify if these changes stay when using the self-consistent brush model, which allows obtaining of the quasistatic Young's modulus with an error of just a few percent. 24,25 Here we observed that bladder epithelial cancer cells are still softer than nonmalignant ones, which qualitatively agrees with the previously reported observations.…”

Section: ■ Results and Discussionsupporting

confidence: 92%

“…Although it was previously reported that malignant cells were softer than nonmalignant, ignoring the pericellular layer, it substantially changes the derived values of the quasistatic Young’s modulus . Thus, it was interesting to verify if these changes stay when using the self-consistent brush model, which allows obtaining of the quasistatic Young’s modulus with an error of just a few percent. , Here we observed that bladder epithelial cancer cells are still softer than nonmalignant ones, which qualitatively agrees with the previously reported observations.…”

Section: Discussionsupporting

confidence: 91%

“…AFM is a suitable method for examining the physical properties of cell glycocalyx. 21,24,25,45 First, we study the mechanical properties of the cell body. The link between the mechanical properties of cells and the Thus, we started the experiments to characterize the actin content and filament organization in nonmalignant HCV29 and transitional cell carcinoma TCCSUP cells.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Mechanical Way To Study Molecular Structure of Pericellular Layer

Makarova

Lekka²,

Gnanachandran³

et al. 2023

ACS Appl. Mater. Interfaces

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Atomic force microscopy (AFM) has been used to study the mechanical properties of cells, in particular, malignant cells. Softening of various cancer cells compared to their nonmalignant counterparts has been reported for various cell types. However, in most AFM studies, the pericellular layer was ignored. As was shown, it could substantially change the measured cell rigidity and miss important information on the physical properties of the pericellular layer. Here we take into account the pericellular layer by using the brush model to do the AFM indentation study of bladder epithelial bladder nonmalignant (HCV29) and cancerous (TCCSUP) cells. It allows us to measure not only the quasistatic Young’s modulus of the cell body but also the physical properties of the pericellular layer (the equilibrium length and grafting density). We found that the inner pericellular brush was longer for cancer cells, but its grafting density was similar to that found for nonmalignant cells. The outer brush was much shorter and less dense for cancer cells. Furthermore, we demonstrate a method to convert the obtained physical properties of the pericellular layer into biochemical language better known to the cell biology community. It is done by using heparinase I and neuraminidase enzymatic treatments that remove specific molecular parts of the pericellular layer. The presented here approach can also be used to decipher the molecular composition of not only pericellular but also other molecular layers.

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Section: ■ Results and Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanical Way To Study Molecular Structure of Pericellular Layer

Makarova

Lekka²,

Gnanachandran³

et al. 2023

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…More detail, including the robustness and error analysis of this model, was presented in ref. 27, where the high robustness of the model and the relatively low error of the derived parameters were reported. Each force curve derived from the AFM is a function of the cantilever deflection ( d ) with respect to the vertical displacement of the AFM scanner ( Z ).…”

Section: Methodsmentioning

confidence: 89%

“…As was recently shown, the errors in the definition of an effective Young's modulus due to the possible uncertainties of the model and experimental data are within 4%, which is less than the error, for example, due to the typical uncertainty in the spring constant of the AFM cantilever. 27 Knowledge of the PB layer has a big biological significance. This layer surrounds neurofilaments to maintain interfilamentous spacing.…”

Section: Introductionmentioning

confidence: 99%