Cell Mechanotransduction With Piconewton Forces Applied by Optical Tweezers

Falleroni, Fabio; Torre, Vincent; Cojoc, D.

doi:10.3389/fncel.2018.00130

Cited by 57 publications

(48 citation statements)

References 36 publications

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“…The proposed NOTOD is not only compact, having high optical efficiency, but also a flexible tool for optical method to control trapped bead in space and to stretch different kind of DNA molecules. Using organic dye with high nonlinearity, the optical trap efficiency of NOTOD is high enough, which can trap nanoparticle [21,22] and stretch DNA molecule [44][45][46][47], instead of using ultrafast pulsed laser.…”

Section: Discussionmentioning

confidence: 99%

Nonlinear Optical Tweezers As an Optical Method for Controlling Particles with High Trap Efficiency

Quy¹

2019

Comm. in Phys.

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Optical tweezers have seen as an essential tool for the manipulation dielectric microparticles and nanoparticles due to its non-contact action and high resolution of optical force. Up to now, there has been a lot of optical tweezers applications in the fields of biophysics, chemistry, medical science and nanoscience. Recently, optical tweezers have been theoretically and experimentally developing for the nanomechanical characterization of various kinds of biological cells. The configuration of optical tweezers has been day after day improving to enhance the trapping efficiency, spatial and temporal resolution and easy to control trapped objects. In common trend of optical tweezers improvements, we will discuss in detail of the several configurations of nonlinear optical tweezers using nonlinear materials as the added lens. We will also address the advantages of nonlinear optical tweezers, such as enhance optical efficiency, reduce trapping region, simplify controlling all-optical method. Finally, we present discussions about the specific properties of nonlinear optical tweezers used for stretch DNA molecule as example and an ideal to improve nonlinear optical tweezers using thin layer of organic dye proposed for going time.

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Section: Discussionmentioning

confidence: 99%

Nonlinear Optical Tweezers As an Optical Method for Controlling Particles with High Trap Efficiency

Quy¹

2019

Comm. in Phys.

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“…Different configurations have been developed to perform nanoindentation with optical tweezers on cells. Indentation can be performed axially by moving the sample through a piezoelectric stage against a trapped dielectric microsphere (Coceano et al 2016;Yousafzai et al 2016), or by moving the trap towards the cell either in a linear (Dy and Sugiura 2013) or in an oscillatory way (Falleroni et al 2018). Another possibility is to use a lateral indentation approach, where the sample or the trap is moved along the image plane against a perpendicular cell membrane (Zhou et al 2014).…”

Section: Nanoindentationmentioning

confidence: 99%

“…It has been recently demonstrated that cellular calcium transients can be precisely induced in mouse neuroblastoma NG108-15 cells with a periodic mechanical stimulation method employing an oscillatory optical trap to apply gentle forces perpendicularly to the cell membrane (Falleroni et al 2018). The induced calcium transients depended on the stimulus strength: the mechanical forces exerted in experiments had maximum values in the range 10-18 pN and induced detectable changes of intracellular Ca 2+ ; a decrease in the trap stiffness by a factor of 2, led to maximum forces in the range 4-10 pN, and no calcium activation was observed (Falleroni et al 2018). Besides force-triggered signals, optical tweezers can be used to trigger specific signals with high spatio-temporal resolution by positioning ligand-coated beads on the cell membrane.…”

Section: Measuring Mechanotransduction Signals In Living Cellsmentioning

confidence: 99%

Probing force in living cells with optical tweezers: from single-molecule mechanics to cell mechanotransduction

Arbore¹,

Perego²,

Σεργίδης³

et al. 2019

Biophys Rev

112

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The invention of optical tweezers more than three decades ago has opened new avenues in the study of the mechanical properties of biological molecules and cells. Quantitative force measurements still represent a challenging task in living cells due to the complexity of the cellular environment. Here, we review different methodologies to quantitatively measure the mechanical properties of living cells, the strength of adhesion/receptor bonds, and the active force produced during intracellular transport, cell adhesion, and migration. We discuss experimental strategies to attain proper calibration of optical tweezers and molecular resolution in living cells. Finally, we show recent studies on the transduction of mechanical stimuli into biomolecular and genetic signals that play a critical role in cell health and disease.

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“…This is achieved by tethering polystyrene beads to cell membranes, which can be manipulated when subjected to focused light. Using this technique, a recent study highlighted how mouse neuroblastoma cells respond to forces ranging from 5 to 20 pN by activation of Ca 2+ ion channels at defined force thresholds (Falleroni et al, 2018). Optical tweezers have been largely applied and optimized to 2D single cell scenarios and thus may prove less attractive to mechanically stimulate more complex 3D multicellular tissues and organoids.…”

Section: Extrinsic Control Of Cellular Forces Through Motorized Devicmentioning

confidence: 99%

Nanoparticles as Versatile Tools for Mechanotransduction in Tissues and Organoids

Fattah

Ranga

2020

Front. Bioeng. Biotechnol.

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Organoids are 3D multicellular constructs that rely on self-organized cell differentiation, patterning and morphogenesis to recapitulate key features of the form and function of tissues and organs of interest. Dynamic changes in these systems are orchestrated by biochemical and mechanical microenvironments, which can be engineered and manipulated to probe their role in developmental and disease mechanisms. In particular, the in vitro investigation of mechanical cues has been the focus of recent research, where mechanical manipulations imparting local as well as large-scale mechanical stresses aim to mimic in vivo tissue deformations which occur through proliferation, folding, invagination, and elongation. However, current in vitro approaches largely impose homogeneous mechanical changes via a host matrix and lack the required positional and directional specificity to mimic the diversity of in vivo scenarios. Thus, while organoids exhibit limited aspects of in vivo morphogenetic events, how local forces are coordinated to enable large-scale changes in tissue architecture remains a difficult question to address using current techniques. Nanoparticles, through their efficient internalization by cells and dispersion through extracellular matrices, have the ability to provide local or global, as well as passive or active modulation of mechanical stresses on organoids and tissues. In this review, we explore how nanoparticles can be used to manipulate matrix and tissue mechanics, and highlight their potential as tools for fate regulation through mechanotransduction in multicellular model systems.

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Cell Mechanotransduction With Piconewton Forces Applied by Optical Tweezers

Cited by 57 publications

References 36 publications

Nonlinear Optical Tweezers As an Optical Method for Controlling Particles with High Trap Efficiency

Nonlinear Optical Tweezers As an Optical Method for Controlling Particles with High Trap Efficiency

Probing force in living cells with optical tweezers: from single-molecule mechanics to cell mechanotransduction

Nanoparticles as Versatile Tools for Mechanotransduction in Tissues and Organoids

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