Cell-mediated antiviral response to equine herpesvirus type 1 demonstrated in a murine infection model by means of adoptive transfer of immune cells

Lila, Mohd Azmi Mohd; Field, Hugh J.

doi:10.1099/0022-1317-74-2-275

Cited by 27 publications

(15 citation statements)

References 9 publications

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“…These findings can be compared to observations in the natural host, which appears to retain limited protection against re-infection, but for a short duration (Allen & Bryans, 1986). Recently, we have obtained evidence that the murine immune response to EHV-1, like that of the natural host, comprises both antibody and T cell responses, but that cell-mediated immunity predominates in the control or otherwise of infection (Azmi & Field, 1993).…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of a Thymidine Kinase-Deficient Mutant of Equine Herpesvirus 1 in Mice and Specific Pathogen-Free Foals

Slater

Gibson

Field

1993

Journal of General Virology

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Both intranasal (i.n.) and intracerebral (i.c.) inoculation of mice with wild-type equine herpesvirus type 1 (wt EHV-1) caused clinical signs and mortality. Virus could be recovered from target organs (turbinates, lungs and blood) for several days. By contrast, the thymidine kinase (TK)-deficient deletion mutant PR1 produced markedly less clinical disease following both i.n. and i.c. inoculation, and, in particular, no mortality occurred.PR1 did, however, establish productive infections following either route of inoculation. High titres of virus were recovered from target organs although virus did not persist for as long as wt EHV-1 and no viraemia was detected. Primary i.n. infection of mice with either wt EHV-1 or PR1 protected against subsequent challenge with wt EHV-1 5 weeks later. I.n. inoculation of specific pathogen-free (EHV-free) foals with PR1 produced results similar to those observed after infection of mice.Clinical signs were milder than for wt EHV-1 and pyrexia was short-lived or absent. PR1 could be recovered from nasal mucus at high titres but it persisted for only 5 days post-infection compared to 11 days in the case of wt EHV-1. No viraemia was detected in foals infected with PR1. On challenge with wt EHV-1, foals given a primary infection with the mutant were partially protected; but a viraemia with a TK + EHV-1 was observed. These results demonstrate that our TK mutant PR1 is markedly less pathogenic than wt EHV-1, despite being able to replicate in the host. The use of TK-deficient mutants of EHV-1 as potential vaccines in the horse is discussed.

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Section: Discussionmentioning

confidence: 99%

Pathogenicity of a Thymidine Kinase-Deficient Mutant of Equine Herpesvirus 1 in Mice and Specific Pathogen-Free Foals

Slater

Gibson

Field

1993

Journal of General Virology

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“…= mean cpm of triplicate cultures from the same group (medium alone) Cells were obtained from pooled splenic lymphocytes from three mice in each group and stimulated with either heat inactivated (56 °C for 30 min) semi-purified EHV-1 pelleted from EHV-1 infected RK-13 cell-supernatant at 28000 rpm for 2 h or mock infected RK cell lysates (results in brackets). Stimulation experiments were repeated twice and the data represent the result of one such experiment fND Not done gDelayed type hypersensitivity (DTH) was assessed as described [7]. The results represent increase in ear thickness 24h post inoculation of heat killed purified EHV-1 antigen in 20gl volume (1 x 1 0 6 erstwhile pfu) in right ear pinna.…”

Section: Tewari Et Almentioning

confidence: 99%

Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model

Tewari

Nair

Ungria

et al. 1995

Archives of Virology

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The glycoprotein C (gC) gene of equine herpesvirus-1 (EHV-1) was expressed in insect cells by a recombinant baculovirus as several products with apparent molecular weights of 66 kDa-80 kDa. The baculovirus EHV-1 gC products were recognised by monoclonal antibody and by EHV-1 convalescent equine sera, indicating conservation of antigenic determinants and confirming this glycoprotein as a target for the equine immune system. Mice immunized with recombinant EHV-1 gC showed accelerated clearance of EHV-1 from respiratory tissues following intranasal challenge. Virus clearance was accompanied by virus specific antibodies and by cell mediated immune responses measured by a delayed type hypersensitivity reaction and lymphocyte stimulation by killed EHV-1 as antigen.

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“…Adoptive transfer of CIL-stimulated CTL Splenocytes isolated as described previously 43 were resuspended in Hank's balanced salt solution containing 1% FCS, and stimulated with 5 g/ml PHA and 100 U/ml recombinant mouse IL-2 for 4 to 5 days. Animals bearing tumors, established for 14-20 days, received both intratumoral and intraperitoneal (i.p.)…”

Section: Immunohistochemistrymentioning

confidence: 99%

Taking lessons from dendritic cells: multiple xenogeneic ligands for leukocyte integrins have the potential to stimulate anti-tumor immunity

et al. 1999

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Expression of large numbers of different costimulatory intetumor-specific cytolytic T cell (CTL) activity involving both grin ligands (CILs) attributes dendritic cells with an ability the perforin and Fas-ligand pathways. Adoptive transfer of to induce primary anti-tumor immune responses. Here, we splenocytes from cured mice rapidly cleared established show that optimized gene transfer of the xenogeneic tumors in recipients. The mechanism for CIL-mediated (human) CILs VCAM-1, MAdCAM-1 and ICAM-1 causes immunity is unknown, but may involve CTL-facilitated rapid and complete rejection of established mouse EL-4 tumor lysis, since CTLs were generally twice as efficient at tumors, and generates prolonged systemic anti-tumor killing CIL-transfected tumor cells than parental tumor cells. immunity; whereas human E-cadherin weakly slows tumor Optimized CIL-based gene therapy may provide an growth. In each case the immune response was mediated approach to complement or replace conventional DC by CD8+ T cells and NK cells, accompanied by augmented adoptive cell therapy for suppressing tumor growth.

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Cell-mediated antiviral response to equine herpesvirus type 1 demonstrated in a murine infection model by means of adoptive transfer of immune cells

Cited by 27 publications

References 9 publications

Pathogenicity of a Thymidine Kinase-Deficient Mutant of Equine Herpesvirus 1 in Mice and Specific Pathogen-Free Foals

Pathogenicity of a Thymidine Kinase-Deficient Mutant of Equine Herpesvirus 1 in Mice and Specific Pathogen-Free Foals

Immunization with glycoprotein C of equine herpesvirus-1 is associated with accelerated virus clearance in a murine model

Taking lessons from dendritic cells: multiple xenogeneic ligands for leukocyte integrins have the potential to stimulate anti-tumor immunity

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