Cell-mediated cytotoxicity in isogeneic ginbuna crucian carp

Nakanishi, Teruyuki; Okamoto, Nobuaki

doi:10.1006/fsim.1998.0191

Cited by 21 publications

(14 citation statements)

References 26 publications

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“…In rainbow trout LMP2, LMP2/d, TAP1A, and TAP2B also have been located in the class Ia locus (20), which suggests that class I-dependent Ag processing and presentation could follow the same pathway as in mammals. In cloned goldfish, the involvement of a T cell response during viral infection was recently suggested by the observation of a specific cell-mediated lysis of virus-infected syngenic target cells (22,23). However, the effector cells were not unequivocally identified as T cells.…”

Section: Iral Hemorrhagic Septicemia Virus (Vhsv)mentioning

confidence: 99%

Rhabdovirus Infection Induces Public and Private T Cell Responses in Teleost Fish

Boudinot

Boubekeur

Benmansour

2001

The Journal of Immunology

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Many viruses induce a strong T cell response that contributes to the elimination of infected cells presenting viral peptides by MHC molecules. The structure and expression of genes encoding molecules homologous to mammalian αβ TCRs have been recently characterized in rainbow trout and in several teleost species, but the αβ T cell response against pathogens has not been directly demonstrated. To study the modifications of the T cell repertoire during an acute viral infection in rainbow trout, we adapted the immunoscope methodology, which consists of spectratyping the complementarity-determining region 3 length of the TCRβ chain. We showed that the naive T cell repertoire is polyclonal and highly diverse in the naive rainbow trout. Using viral hemorrhagic septicemia virus (VHSV), which provokes an acute infection in rainbow trout, we identified skewed complementarity-determining region 3 size profiles for several VβJβ combinations, corresponding to T cell clonal expansions during primary and secondary response to VHSV. Both public and private T cell expansions were shown by immunoscope analysis of spleen cells from several infected individuals of a rainbow trout clone sharing the same genetic background. The public response to VHSV consisted of expansion of Vβ4Jβ1 T cell, which appeared early during the primary response and was strongly boosted during the secondary response.

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Section: Iral Hemorrhagic Septicemia Virus (Vhsv)mentioning

confidence: 99%

Rhabdovirus Infection Induces Public and Private T Cell Responses in Teleost Fish

Boudinot

Boubekeur

Benmansour

2001

The Journal of Immunology

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“…Nakanishi and Okamoto reviewed that fish CTL was MHC class I restricted [22]. The CTL recognized and killed the virus-infected syngeneic cells but failed to recognize and kill the virus-infected allogeneic cells.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic CD8α+ leucocytes have heterogeneous features in antigen recognition and class I MHC restriction in grouper

Chang

Kai

Chi

et al. 2011

Fish & Shellfish Immunology

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“…In vitro assays for allospecific cytotoxicity have also been established using clonal and nonclonal catfish cell lines (33,34) or clonal rainbow trout (35). Autologous cell-mediated specific lysis of virus-infected syngenic target cells has also been reported in cloned goldfish (36,37). Finally, both public and private T cellspecific expansions have been observed in rainbow trout during secondary immune responses to viral hemorrhagic septicemia virus (VHSV), 3 a fish rhabdovirus, using TCR-␤ CDR3-length spectratyping (38).…”

mentioning

confidence: 99%

Costimulatory Receptors in a Teleost Fish: Typical CD28, Elusive CTLA4

Bernard

Riteau

Hansen

et al. 2006

The Journal of Immunology

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T cell activation requires both specific recognition of the peptide-MHC complex by the TCR and additional signals delivered by costimulatory receptors. We have identified rainbow trout sequences similar to CD28 (rbtCD28) and CTLA4 (rbtCTLA4). rbtCD28 and rbtCTLA4 are composed of an extracellular Ig-superfamily V domain, a transmembrane region, and a cytoplasmic tail. The presence of a conserved ligand binding site within the V domain of both molecules suggests that these receptors likely recognize the fish homologues of the B7 family. The mRNA expression pattern of rbtCD28 and rbtCTLA4 in naive trout is reminiscent to that reported in humans and mice, because rbtCTLA4 expression within trout leukocytes was quickly up-regulated following PHA stimulation and virus infection. The cytoplasmic tail of rbtCD28 possesses a typical motif that is conserved in mammalian costimulatory receptors for signaling purposes. A chimeric receptor made of the extracellular domain of human CD28 fused to the cytoplasmic tail of rbtCD28 promoted TCR-induced IL-2 production in a human T cell line, indicating that rbtCD28 is indeed a positive costimulator. The cytoplasmic tail of rbtCTLA4 lacked obvious signaling motifs and accordingly failed to signal when fused to the huCD28 extracellular domain. Interestingly, rbtCTLA4 and rbtCD28 are not positioned on the same chromosome and thus do not belong to a unique costimulatory cluster as in mammals. Finally, our results raise questions about the origin and evolution of positive and negative costimulation in vertebrate immune systems.

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Cell-mediated cytotoxicity in isogeneic ginbuna crucian carp

Cited by 21 publications

References 26 publications

Rhabdovirus Infection Induces Public and Private T Cell Responses in Teleost Fish

Rhabdovirus Infection Induces Public and Private T Cell Responses in Teleost Fish

Cytotoxic CD8α+ leucocytes have heterogeneous features in antigen recognition and class I MHC restriction in grouper

Costimulatory Receptors in a Teleost Fish: Typical CD28, Elusive CTLA4

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