Cell-mediated cytotoxicity in rainbow trout, Oncorhynchus mykiss, infected with viral haemorrhagic septicaemia virus

Utke, Katrin; Bergmann, Sven; Lorenzen, Niels; Köllner, Bernd; Ototake, Mitsuru; Fischer, Uwe

doi:10.1016/j.fsi.2006.04.008

Cited by 116 publications

(75 citation statements)

References 50 publications

(61 reference statements)

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“…Surprisingly, they found that specific CMC activity mediated by CTLs was produced much earlier than the innate activity, in sharp contrast to all the information at this respect. Strikingly, the NKEF gene expression followed the same time-profile than the CTL activity but in the case of CD8 was opposite, adding more controversy to these data (Utke et al, 2007). Furthermore, trout vaccination with VHSV DNA vaccines also elicited CMC activity against MHC I-matched infected cells, suggesting a role for CTLs (Utke et al, 2008).…”

Section: Cmc Activity Referencesmentioning

confidence: 96%

“…Thus, ginbuna crucian carps immunized with hematopoietic necrosis virus (CHNV) specifically killed CHNVinfected syngeneic cells in a viral antigen and MHC I-restricted manner (Somamoto et al, 2000, increased the TCR and CD8 gene expression (Somamoto et al, 2006) and helped to establish virus-dependent CTL clones in vitro (Somamoto et al, 2009). In rainbow trout, infection with VHSV greatly elicited specific-and MHC I-matched cytotoxic cells but a non-specific and MHC I-mismatched cytotoxic activity was also found (Fischer et al, 2006;Utke et al, 2007). Surprisingly, they found that specific CMC activity mediated by CTLs was produced much earlier than the innate activity, in sharp contrast to all the information at this respect.…”

Section: Cmc Activity Referencesmentioning

confidence: 99%

“…The transcript level of CD8 gene is related to the CTL presence, abundance and activity. Thus, fish CD8 transcripts are up-regulated by viral and bacterial infections, viral DNA vaccines, scale grafts, poly I:C or mitogens (Byon et al, 2005(Byon et al, 2006Cuesta et al, 2010;Cuesta & Tafalla, 2009;Forlenza et al, 2008;Overturf & LaPatra, 2006;Somamoto et al, 2005Somamoto et al, , 2006Utke et al, 2007;Xu et al, 2011). In some studies, these CD8 gene levels have been correlated with increased CTL activity.…”

Section: Modulation Of the Fish Cytotoxic Activitymentioning

confidence: 99%

“…Finally, other genes related to the cytotoxic activity have received less attention. In this category, the natural killer enhancing factor (NKEF), which increase the cytotoxic activity in humans but its role is unknown in fish, is up-regulated by viral infections and DNA vaccines (Cuesta & Tafalla, 2009;Ordás et al, 2011;Utke et al, 2007) while granulysin, which is secreted together to granzymes and lyses target cells, gene is up-regulated in CD8 + lymphocytes by mitogen stimulation (Takizawa et al, 2011). Further studies are needed to clearly state the gene expression with either innate or specific cytotoxic activity in fish.…”

Section: Modulation Of the Fish Cytotoxic Activitymentioning

confidence: 99%

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Fighting Virus and Parasites with Fish Cytotoxic Cells

Esteban¹,

Meseguer²,

Cuesta³

2012

Health and Environment in Aquaculture

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Section: Cmc Activity Referencesmentioning

confidence: 96%

Section: Cmc Activity Referencesmentioning

confidence: 99%

Section: Modulation Of the Fish Cytotoxic Activitymentioning

confidence: 99%

Section: Modulation Of the Fish Cytotoxic Activitymentioning

confidence: 99%

See 2 more Smart Citations

Fighting Virus and Parasites with Fish Cytotoxic Cells

Esteban¹,

Meseguer²,

Cuesta³

2012

Health and Environment in Aquaculture

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“…Both antiviral CTL and alloantigenspecific CTL have been studied in teleosts (21,22). For example, studies in ginbuna carp and rainbow trout have provided evidence of MHC-restricted killing of virus-infected cells after immunization, and the observed cytotoxic activity correlated with an increased expression of message for CD8a (23)(24)(25)(26). In catfish, the successful cloning of alloantigen-specific CTL from the PBL of a fish immunized with allogeneic 3B11 B cells provided the first direct evidence of alloantigen-specific T cells in fish.…”

mentioning

confidence: 99%

A Leukocyte Immune-Type Receptor Subset Is a Marker of Antiviral Cytotoxic Cells in Channel Catfish, Ictalurus punctatus

Taylor

Moulana

Stuge

et al. 2016

The Journal of Immunology

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Channel catfish, Ictalurus punctatus, leukocyte immune type receptors (LITRs) represent a multigene family that encodes Ig superfamily proteins that mediate activating or inhibitory signaling. In this study, we demonstrate the use of mAb CC41 to monitor viral cytotoxic responses in catfish and determine that CC41 binds to a subset of LITRs on the surface of catfish clonal CTLs. Homozygous gynogenetic catfish were immunized with channel catfish virus (CCV)–infected MHC-matched clonal T cells (G14D-CCV), and PBL were collected at various times after immunization for flow cytometric analyses. The percentage of CC41+ cells was significantly increased 5 d after primary immunization with G14D-CCV and at 3 d after a booster immunization as compared with control fish only injected with G14D. Moreover, CC41+ cells magnetically isolated from the PBL specifically killed CCV-infected targets as measured by 51Cr release assays and expressed messages for CD3γδ, perforin, and at least one of the CD4-like receptors as analyzed by RNA flow cytometry. When MLC effector cells derived from a G14D-CCV–immunized fish were preincubated with CC41 mAb, killing of G14D-CCV targets was reduced by ∼40%, suggesting that at least some LITRs have a role in target cell recognition and/or cytotoxicity. The availability of a LITR-specific mAb has allowed, to our knowledge for the first time, functional characterization of LITRs in an autologous system. In addition, the identification of an LITR subset as a cytotoxic cell marker will allow for more effective monitoring of catfish immune responses to pathogens.

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Immunology of Fish

Fletcher¹,

Secombes²

2010

Encyclopedia of Life Sciences

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Innate immunity provides some form of defence against pathogens in all multicellular organisms but with cartilaginous and bony fish, although the lowest group of jawed vertebrates, the addition of a classic adaptive immune response becomes apparent. This provides the refinements of specificity for antigen recognition and memory. The T‐ and B lymphocytes are the effector leucocytes, acquiring their antigen‐specific receptors (immunoglobulin for B cells and T‐cell receptor for T cells) in the anterior kidney (bone marrow being absent) and thymus. The B cells are responsible for the production of antibodies that function as different immunoglobulin classes, whereas subsets of T lymphocytes are capable of killing target cells or helping with B‐ and other T‐cell functions. Knowledge of these mechanisms is important for the use and design of vaccines, now so essential for the aquaculture industry, whereas studies of fish immunology contribute to the understanding of the evolution of adaptive immunity. Key concepts: Fish possess innate and adaptive immune responses, with classical adaptive immunity present only in jawed vertebrates. Adaptive immunity allows memory of prior exposure to foreign molecules (antigens), so that on subsequent contact protective mechanisms are more rapidly mobilized: the basis of vaccination. Lymphocytes mediate adaptive immunity and have surface receptors that recognize antigens, initiating the production of circulating antibodies and the activation of cell‐mediated responses. The functions of the immune system are similar in fish and mammals although there are differences due to the aquatic physiology and ectothermic nature of fish that provide evolutionary interest. Leucocyte–antigen interactions take place in immune sites, such as the kidneys, spleen, gills and intestine. Bone marrow and lymph nodes are lacking. Knowledge of fish immunology is proving essential for the development of vaccines for the expanding aquaculture industry.

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Cell-mediated cytotoxicity in rainbow trout, Oncorhynchus mykiss, infected with viral haemorrhagic septicaemia virus

Cited by 116 publications

References 50 publications

Fighting Virus and Parasites with Fish Cytotoxic Cells

Fighting Virus and Parasites with Fish Cytotoxic Cells

A Leukocyte Immune-Type Receptor Subset Is a Marker of Antiviral Cytotoxic Cells in Channel Catfish, Ictalurus punctatus

Immunology of Fish

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