Cell‐mediated immune response <i>in vitro</i>: Independent differentiation of thymocytes into cytotoxic lymphocytes

Wagner, H.

doi:10.1002/eji.1830010620

Cited by 37 publications

(18 citation statements)

References 11 publications

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“…After exposure to either antigens or mitogens (see articles in [4]) previously resting B lymphocytes undergo numerous divisions which lead to the formation of clones of cells. The rate of DNA synthesis, and thus the rate of thymidine uptake, increases between 12 and 23 h after stimulation [ 1, 51.…”

Section: J Anderson* and F Melchersmentioning

confidence: 99%

Cell‐mediated cytotoxicity to trinitrophenyl‐modified syngeneic lymphocytes

1974

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Spleen cells from B10 congenic mouse strains were cultured in vitro by a modification of the Mishell‐Dutton technique with trinitrophenyl (TNP)‐modified syngeneic spleen cells. Five days later, the effector cells generated were incubated with 51 Cr‐labeled unmodified or TNP‐modified spleen or tumor target cells, and the percentage of specific lysis determined. The results obtained using modified syngeneic as well as congenic target cells indicated that although TNP‐modification of the target cells was necessary to obtain lysis, the cytotoxicity was not specific for TNP exclusively, but was primarily directed against modified syngeneic cell surface components which may or may not have included the TNP moiety as an integral part of the recognition unit. A number of criteria were used to demonstrate that the phenomenon was attributed to T cell‐mediated cytotoxicity, and was not due to lymphocyte‐dependent antibody (LDA). These included: (a) failure of TNP‐lysine to block the effector phase; (b) lack of detectable LDA in the culture media; (c) failure of spleen cells from athymic nude donors to generate effector cells; (d) sensitivity of effector cells to rabbit anti‐mouse brain serum; and (e) the generation of effector cells by cortisone‐resistant thymocytes. The specificity observed between the syngeneic TNP‐modified sensitizing and target cells is compatible with the hypothesis that the TNP is altering cell surface proteins which are controlled by genes within distinct regions of the H‐2 major histocompatibility complex, and that these proteins are conformationally changed so as to be immunogenic to syngeneic thymus‐derived lymphocytes.

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Section: J Anderson* and F Melchersmentioning

confidence: 99%

Cell‐mediated cytotoxicity to trinitrophenyl‐modified syngeneic lymphocytes

1974

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“…mouse allograft responses has been shown to be a function of thymus-derived (T) lymphocytes (12,13). It was of interest therefore to investigate whether there exists a heterogeneity within T lymphocytes as obtained from different tissues in regard to their capacity to differentiate into cytotoxic effector cells.…”

Section: Heterogeneity Among T Lymphocytes In Their Capacity To Mountmentioning

confidence: 99%

Synergy During in Vitro Cytotoxic Allograft Responses

Wagner

1973

The Journal of Experimental Medicine

129

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The importance of cell interaction among lymphoid cells in the development of immune responses is well established. In humoral responses bone marrowderived (B) lymphocytes are recognized to be precursors of the cells that release serum antibody (1-4). Thymus-derived (T) lymphocytes, on the other hand, do not develop into antibody-forming cells (5) but play an important role in primary antibody responses by cooperating with B cells (6, 7).In contrast, cellular immunity is thought to be mediated by T lymphocytes, apparently without collaboration with B lymphocytes. However there is increasing evidence to suggest that cooperative interaction occurs among subsets of T lymphocytes (8-11). For example, Cantor and Asofsky proposed that a Ti-T~ cell interaction takes place during the initiation of graft-vs.-host responses in F1 neonates (8,9).Cytotoxic in vitro allograft responses have been shown to be mediated by T lymphocytes (12, 13). As such, they provide a convenient tool for analyzing the cellular requirements necessary for such a response to occur. Experiments therefore were designed to evaluate in vitro the concept of T-T cell interaction in cellular immunity against transplantation antigens. The results obtained are compatible with the idea that two cell types interact with each other in the initiation of such a response. Moreover, evidence is provided that one cell type acts mainly as helper cells allowing precursor cells of cytotoxic lymphocytes (CL) i to be immunized effectively.

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“…Thus experiments were performed to determine whether enriched populations of macrophages could restore the cytotoxic response of lymphocytes. Since PEC populations also contain T lymphocytes, and since the allograft response in vitro is caused by T lymphocytes (29,14), the PEC were treated with AKR anti-OC~H serum and complement before use in culture. Fig.…”

Section: Reconstitution Of the Cytotoxic Response With Peritoneal Exumentioning

confidence: 99%

Cell-Mediated Immune Response in Vitro

Wagner

Feldmann

Boyle

et al. 1972

The Journal of Experimental Medicine

151

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The combined use of in vitro culture techniques together with efficient cell separation methods have revealed that macrophages are essential participants in the initiation of some antibody responses (1-4). The experimental design used was to obtain "purified" (macrophage-depleted) lymphocytes by a cell separation procedure and to demonstrate that these cells could not respond to a particular antigen unless macrophages were also present. Several mechanisms of macrophage function during the induction of the humoral immune response have been proposed. Since it was found that in vitro responses to particulate antigens, such as whole erythrocytes, required the participation of macorphages, whereas responses to smaller sized antigens, such as "soluble sheep red blood cell antigen" (3), or polymeric flagellin of Salmonella adelaide (POL) 1 did not, it was proposed that the role of macrophages may therefore be merely to reduce antigenic particles to a smaller and more immunogenic size. Experiments demonstrating that macrophage supernatants enhance responses to erythrocytes (5, 6) are consistent with this simple mechanism of macrophage function. Recently it was found that the immune response to thymus-dependent antigens, even those of small size, such as monomeric flagellin (MON), dinitrophenylated MON (DNP MON), or DNP fowl gamma globulin (DNP F~G), all required the presence of macrophages, whereas responses to the same antigenic determinants (DNP) on polymeric carriers (i.e. POL or DNP POL) which are thymus independent were also found to be

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Cell‐mediated immune response in vitro: Independent differentiation of thymocytes into cytotoxic lymphocytes

Cited by 37 publications

References 11 publications

Cell‐mediated cytotoxicity to trinitrophenyl‐modified syngeneic lymphocytes

Cell‐mediated cytotoxicity to trinitrophenyl‐modified syngeneic lymphocytes

Synergy During in Vitro Cytotoxic Allograft Responses

Cell-Mediated Immune Response in Vitro

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