Cell-mediated immune response is better preserved by laparoscopy than laparotomy

Gitzelmann, Christopher A.; Mendoza-Sagaon, Mario; Talamini, Mark A.; Ahmad, Syed A.; Pegoli, Walter; Paidas, Charles N.

doi:10.1067/msy.2000.101152

Cited by 90 publications

(46 citation statements)

References 15 publications

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“…The findings suggest that both groups with air exposure of the peritoneal cavity have more pronounced immune alteration, a finding that was also observed in our former studies [22,23]. This finding may closely be linked to the suppressed recall immunity in terms of delayed type hypersensitivity during the four postoperative days in mice exposed to laparotomy observed earlier [8].…”

Section: Discussionsupporting

confidence: 73%

“…We used a relatively late time point of 48 h post-intervention, as delayed-type hypersensitivity in mice was suppressed at that time point after laparotomy, but not after laparoscopy [8]. Our aim was to assess eventual differences in cytokine patterns and T cell subpopulations (cytotoxic T cells, T helper cells and regulatory T cells) after an air versus CO 2 pneumoperitoneum.…”

mentioning

confidence: 99%

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Immune cell populations and cytokine production in spleen and mesenteric lymph nodes after laparoscopic surgery versus conventional laparotomy in mice

et al. 2012

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Section: Discussionsupporting

confidence: 73%

mentioning

confidence: 99%

Immune cell populations and cytokine production in spleen and mesenteric lymph nodes after laparoscopic surgery versus conventional laparotomy in mice

et al. 2012

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“…Gitzelmann et al [5] have previously demonstrated postoperative preservation of the immune function after laparoscopic surgery, as compared with laparotomy, in a murine model [5]. Cell-mediated delayed-type hypersensitivity was preserved after CO 2 pneumoperitoneum and sham extraperitoneal incision, as compared with open laparotomy, postoperative days 1 and 2 as well as postoperative days 4 and 5.…”

mentioning

confidence: 92%

“…Cell-mediated delayed-type hypersensitivity was preserved after CO 2 pneumoperitoneum and sham extraperitoneal incision, as compared with open laparotomy, postoperative days 1 and 2 as well as postoperative days 4 and 5. Tissue trauma was shown not to be a relevant factor in this study [5], whereas in other experiments, immunosuppression correlated directly with the surgical tissue trauma [1,6].…”

mentioning

confidence: 96%

Early peritoneal macrophage function after laparoscopic surgery compared with laparotomy in a mouse mode

Moehrlen¹,

Schwöbel

Ernst³

et al. 2005

Surg Endosc

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“…Using animal experiments, some researchers have studied the effect of CO 2 pneumoperitoneum on tumor relapse and metastasis after gastrointestinal tumor surgery and now believe that laparoscopic surgery may influence the relapse and metastasis of gastric and colorectal cancers by affecting the body’s immune system. Compared to laparotomy, laparoscopic surgery is generally recognized to create less trauma or stress on the body and less systemic immunosuppression, and these patients therefore recover more quickly after surgery [16,17,18]. However, because there are many animal models used to study the effect of CO 2 pneumoperitoneum on peritoneal immunity and very few clinical studies, conclusions are not consistent.…”

Section: Discussionmentioning

confidence: 83%

Effects of CO<sub>2</sub> Pneumoperitoneum on Peritoneal Macrophage Function and Peritoneal Metastasis in Mice with Gastric Cancer

Luo

Yu²,

Hao³

et al. 2011

Eur Surg Res

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Background: Whether laparoscopy with CO₂ pneumoperitoneum affects the peritoneal metastasis of gastric cancer is a pressing question. In light of the important impact change in peritoneal macrophage function has on the peritoneal metastasis of gastric cancer, this study investigated the change in peritoneal macrophage function in gastric cancer in the CO₂ pneumoperitoneum environment, as well as its effect on the peritoneal metastasis of gastric cancer. Methods: An orthotopic transplantation model of murine forestomach carcinoma was established using the 615 mouse line. The mice bearing tumors were randomly divided into four groups (30 mice each group): anesthesia alone, laparotomy, mini-laparotomy, and CO₂ insufflation. After the operation, peritoneal macrophages were collected from 6 mice in each group and cultured. The phagocytosis of neutral red by macrophages and the levels of NO, TNF-α, IL-10, and VEGF produced by macrophages were measured after 12, 24, 48, and 72 h of culture. The remaining mice were observed after 2 weeks for the rate of peritoneal metastasis of forestomach carcinoma cells and the total weight of implanted nodules. Results: In the laparotomy group, 4 mice died intraoperatively and 2 died in the CO₂ insufflation group. The uptake of neutral red by peritoneal macrophages and the levels of NO, TNF-α, IL-10, and VEGF secreted by peritoneal macrophages in the laparotomy group and mini-laparotomy group after 12 h of culture were all significantly higher than those in the anesthesia-alone group (p < 0.05). The corresponding levels in the CO₂ insufflation group after 12 h were all significantly lower than those in the anesthesia-alone group (p < 0.05). There were no significant differences among the four groups at 24, 48, and 72 h after culture. Comparing with those in the laparotomy group, the uptake of neutral red by peritoneal macrophages and the levels of NO, TNF-α, IL-10, and VEGF secreted by peritoneal macrophages in the CO₂ insufflation group were all significantly lower after 12 h of culture (p < 0.05), but did not differ significantly at 24, 48, and 72 h of culture (p > 0.05), and did not differ significantly in the mini-laparotomy group at all the time (p > 0.05). The rate of peritoneal metastasis of mouse forestomach carcinoma was 50% in the laparotomy group, 45.83% in the mini-laparotomy group, and 45.45% in the CO₂ insufflation group; this difference was not statistically significant (p > 0.05). The total weight of implanted nodules of mouse forestomach carcinoma was 1.02 ± 0.38 g in the laparotomy group, 0.97 ± 0.41 g in the mini-laparotomy group, and 0.93 ± 0.45 g in the CO₂ insufflation group, which was not a statistically significant difference (p > 0.05). Conclusion: CO₂ pneumoperitoneum neither significantly changes the phagocytosis and cytokine secretion functions of peritoneal macrophages in gastric cancer-bearing mice nor significantly promotes peritoneal metastasis of gastric ...

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Cell-mediated immune response is better preserved by laparoscopy than laparotomy

Cited by 90 publications

References 15 publications

Immune cell populations and cytokine production in spleen and mesenteric lymph nodes after laparoscopic surgery versus conventional laparotomy in mice

Immune cell populations and cytokine production in spleen and mesenteric lymph nodes after laparoscopic surgery versus conventional laparotomy in mice

Early peritoneal macrophage function after laparoscopic surgery compared with laparotomy in a mouse mode

Effects of CO<sub>2</sub> Pneumoperitoneum on Peritoneal Macrophage Function and Peritoneal Metastasis in Mice with Gastric Cancer

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