Cell membrane effects of some common biocides

Al-Adham, Ibrahim; Dinning, Anthony Joseph; Eastwood, Ian M.; Austin, P. W.; Collier, Phillip J.

doi:10.1038/sj.jim.2900554

Cited by 44 publications

(27 citation statements)

References 23 publications

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“…Those electrostatic bonds create stress in the cell wall, leading to cell lysis and death. QACs also cause cell death by protein denaturation, disruption of cell wall permeability and reduction of the normal intake of nutrients into the cell [1,4]. Cetyltrimethyl ammonium bromide (CTAB) is a QAC that is considered to rupture the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

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Comparative antibacterial potential of selected aldehyde-based biocides and surfactants against planktonic Pseudomonas fluorescens

Simões

Pereira

Machado

et al. 2006

J IND MICROBIOL BIOTECHNOL

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The antimicrobial efficacy of two aldehyde-based biocides (glutaraldehyde, GTA, and ortho-phthalaldehyde, OPA) and two surfactants (cetyltrimethyl ammonium bromide, CTAB, and sodium dodecyl sulphate, SDS) was tested against planktonic Pseudomonas fluorescens. The antimicrobial effects were evaluated by respiratory activity as a measure of the oxygen uptake rate, adenosine triphosphate (ATP) release, outer membrane proteins (OMP) expression and cellular colour changes. The results were compared with the bacterial characteristics without chemical treatment. Tests in the presence of bovine serum albumin (BSA), in order to mimic a disinfection process in the real situation under dirty conditions, were performed according to the European Standard EN-1276. P. fluorescens was completely inactivated with OPA (minimum bactericidal concentration, MBC = 0.5 mM) and CTAB (MBC = 5 mM) and was resistant to GTA and SDS. Only CTAB promoted cellular disruption and consequent ATP release. The antimicrobial action of the chemicals tested was significantly reduced when BSA was introduced into the bacterial cultures, increasing markedly the MBC values. Additionally, the presence of BSA acted as a disruption protective agent when CTAB was applied and stimulated the bacterial respiratory activity when lower concentrations of SDS were tested. The OMP of the bacterial cells was affected by the application of both surfactants. OMP expression remained unaltered after biocide treatment. Bacterial colour change was noticed after treatment with biocides and surfactants. In summary, P. fluorescens was extremely resistant to GTA and SDS, with antimicrobial action being quenched markedly by the reaction with BSA.

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Section: Introductionmentioning

confidence: 99%

“…Control of microbial growth is required in many microbiologically sensitive environments where wet surfaces provide favourable conditions for proliferation of microorganisms [1,5,22]. The aim of a disinfection process is to reduce the viable microorganisms and to prevent microbial growth on the surfaces [31,32].…”

Section: Introductionmentioning

confidence: 99%

Comparative antibacterial potential of selected aldehyde-based biocides and surfactants against planktonic Pseudomonas fluorescens

Simões

Pereira

Machado

et al. 2006

J IND MICROBIOL BIOTECHNOL

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“…The process of H + dissociation can lead to an acidification of plasmatic membrane surface of microorganism, resulting in H + -ATPase rupture that is required for ATP synthesis (Vattem et al, 2004;Maeda et al, 1999). Besides, it causes intracellular coagulation of cytoplasm constituents, leading the cell to death, or inhibiting its growth (Adham et al, 1998). The Gram positive bacteria are more sensible to these compounds by presenting a simple membrane (Vattem et al, 2004).…”

Section: Antimicrobial Assaysmentioning

confidence: 99%

Cladia aggregata (lichen) from Brazilian northeast: chemical characterization and antimicrobial activity

Martins¹,

Lima²,

Silva³

et al. 2010

Braz. arch. biol. technol.

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“…The centrifugation and the filtration (ϕ: 0.2 µm (Millipore, MA, U.S.A.)) were made before the measurement. 9) Farnesol could contact with bacterial cells for one minute. The volume of bacterial suspension was 4 mL.…”

Section: Detection Of the Changes In The Concentrations Of Potassium mentioning

confidence: 99%

Farnesol-Induced Disruption of the <i>Staphylococcus aureus</i> Cytoplasmic Membrane

Inoué

Togashi

Hamashima

2016

Biological & Pharmaceutical Bulletin

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Farnesol, a sesquiterpene alcohol with an aliphatic carbon chain, inhibited the growth of Staphylococcus aureus and induced the leakage of potassium ions. We investigated the action of farnesol on the cytoplasmic membrane of S. aureus. No ion channels that would account for the loss of potassium ions were detected. Electron paramagnetic resonance measurements suggested that farnesol proceeds into the cytoplasmic membrane of S. aureus cells, where it induces the disordering and eventual disruption of the cytoplasmic membrane. This was supported by the result that the effects of farnesol decreased by the addition of carotenoid which was the stabilizing reagent for the lipid bilayer.Key words farnesol; cell membrane disordering; Staphylococcus aureus Staphylococcus aureus is an opportunistic pathogen. Novel antibiotics against this organism are needed due to the depletion of therapeutic options resulting from the development of resistance to various antibiotics and, consequently, the increasing difficulty of curing nosocomial infections. We previously investigated plant-derived essential oils and their constituents and found that some terpene alcohols with aliphatic carbon chains, such as farnesol, exhibited antibacterial activity against S. aureus.1) The cell membrane of S. aureus was damaged after only a few minutes exposure to farnesol, but the underlying mechanism of this activity was not elucidated.2,3) The aim of the present study was to determine the mechanism of farnesol-induced damage to the cell membrane of S. aureus. The findings of this study could aid in safely and effectively treating infections of this bacterium and enhance efforts to develop more effective medicines for treating S. aureus infections. MATERIALS AND METHODSGeneral Farnesol, carotenoid, 5-doxyl stearic acid (5-NS), 16-doxyl stearic acid methyl ester (16-NMS) and melittin were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). Tetraethyl ammonium chloride and quinine hydroxide were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). S. aureus FDA209P was used as the standard strain. 4)Nitro Blue Tetrazolium (NBT) Assay S. aureus cultures were incubated in brain-heart infusion (BHI) broth overnight, harvested, washed twice with phosphate-buffered saline (PBS), and resuspended in PBS. Farnesol was added to 1 mL of the bacterial suspension (ca. 10 5 colony forming units CFU mL . The cells were then incubated for 30 min at 37°C after the addition of 0.1% NBT solution. The assay was stopped by the addition of 0.1 M hydrochloric acid and the cells were pelleted by centrifugation (1500×g, 10 min, 4°C) and resuspended in dimethyl sulfoxide (600 µL) and PBS (800 µL). The absorbance of the solution at 575 nm was measured using a DU-65 spectrophotometer (Becton, Dickinson and Company, Franklin Lakes, NJ, U.S.A.)Electron Paramagnetic Resonance (EPR) Measurements EPR measurements were made using spin-labeling reagents (5-NS and 16-NMS).5) Spin-labeling reagents were dissolved individually in chloroform (0.1 mg mL −1 ), and a 25-µL ...

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Cell membrane effects of some common biocides

Cited by 44 publications

References 23 publications

Comparative antibacterial potential of selected aldehyde-based biocides and surfactants against planktonic Pseudomonas fluorescens

Comparative antibacterial potential of selected aldehyde-based biocides and surfactants against planktonic Pseudomonas fluorescens

Cladia aggregata (lichen) from Brazilian northeast: chemical characterization and antimicrobial activity

Farnesol-Induced Disruption of the <i>Staphylococcus aureus</i> Cytoplasmic Membrane

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