Cell membrane electroporation modeling: A multiphysics approach

Goldberg, Ezequiel; Suárez, Cecilia; Alfonso, M. Iacono; Marchese, Juan; Soba, Alejandro; Marshall, Guillermo

doi:10.1016/j.bioelechem.2018.06.010

Cited by 52 publications

(26 citation statements)

References 42 publications

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“…Furthermore, the application of an EMF may also induce a local elastic tension through Maxwell's tensor, forcing the membrane to which the field is applied to prolate or oblate, with the result dependent on the properties of the EMF and the mechanical properties of the membrane. 42,43 In summary, in this work, we have explored the potential of 18-GHz EMF treatment to induce transient permeabilization of cell membrane in a mammalian cell model without any detrimental effect to cell viability or metabolism, thereby providing a potential alternative to conventional poration techniques in drug delivery applications.…”

mentioning

confidence: 99%

Exposure to high-frequency electromagnetic field triggers rapid uptake of large nanosphere clusters by pheochromocytoma cells

Perera¹,

Nguyen²,

Dekiwadia³

et al. 2018

IJN

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BackgroundEffects of man-made electromagnetic fields (EMF) on living organisms potentially include transient and permanent changes in cell behaviour, physiology and morphology. At present, these EMF-induced effects are poorly defined, yet their understanding may provide important insights into consequences of uncontrolled (e.g., environmental) as well as intentional (e.g., therapeutic or diagnostic) exposure of biota to EMFs. In this work, for the first time, we study mechanisms by which a high frequency (18 GHz) EMF radiation affects the physiology of membrane transport in pheochromocytoma PC 12, a convenient model system for neurotoxicological and membrane transport studies.Methods and resultsSuspensions of the PC 12 cells were subjected to three consecutive cycles of 30s EMF treatment with a specific absorption rate (SAR) of 1.17 kW kg−1, with cells cooled between exposures to reduce bulk dielectric heating. The EMF exposure resulted in a transient increase in membrane permeability for 9 min in up to 90 % of the treated cells, as demonstrated by rapid internalisation of silica nanospheres (diameter d ≈ 23.5 nm) and their clusters (d ≈ 63 nm). In contrast, the PC 12 cells that received an equivalent bulk heat treatment behaved similar to the untreated controls, showing lack to minimal nanosphere uptake of approximately 1–2 %. Morphology and growth of the EMF treated cells were not altered, indicating that the PC 12 cells were able to remain viable after the EMF exposure. The metabolic activity of EMF treated PC 12 cells was similar to that of the heat treated and control samples, with no difference in the total protein concentration and lactate dehydrogenase (LDH) release between these groups.ConclusionThese results provide new insights into the mechanisms of EMF-induced biological activity in mammalian cells, suggesting a possible use of EMFs to facilitate efficient transport of biomolecules, dyes and tracers, and genetic material across cell membrane in drug delivery and gene therapy, where permanent permeabilisation or cell death is undesirable.

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confidence: 99%

Exposure to high-frequency electromagnetic field triggers rapid uptake of large nanosphere clusters by pheochromocytoma cells

Perera¹,

Nguyen²,

Dekiwadia³

et al. 2018

IJN

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“…This modelling is motivated in part by the recently proposed irreversible EP with high-frequency short bipolar pulses 11,12 that reduces the occurrence of muscle contractions during clinical therapy showing that it is of utmost importance to consider the physical forces that govern cell deformation in order to optimize the EP efficiency. 4 We also note that other authors have proposed interesting 3D models of simple or realistically shaped cells in densely packed tissues. [11][12][13][14]19,20 We use a model characterized by a principle of minimality in many respects.…”

mentioning

confidence: 74%

“…The pursuit of understanding the mechanisms of electrodeformation and electro-poration (EP) of biological cells in suspension or tissue began decades ago 1,2 and the search continues with renewed enthusiasm. 3,4 Understanding the connection between cell electro-deformation and EP is still a relatively unexplored area of research. One of the significant challenges in EP is high molecular weight molecules delivery such as DNA into the living cells.…”

mentioning

confidence: 99%

“…However, most of the existing numerical and analytical studies have tackled the modelling of this phenomenon based on various assumptions and constraints to predict and evaluate cell and tissue EP. [1][2][3][4][5][6][7][8][9][10][11][12][13][14] Typically, EP takes place when the transmembrane potential (TMP) exceeds a threshold V ep above which electrically conductive pores start forming in the membrane. [1][2][3] Experimental estimates for V ep fall in the range of 0.5-1.2 V but theoretical estimates point to V ep ¼ 0.258 V. 15 Most of the existing studies have so far assumed that cell membranes are rigid.…”

mentioning

confidence: 99%

“…24 The simulations use a 50 Â 50 Â 50 lm 3 computational domain with electrically insulated boundary conditions for the y-z and x-z planes (conservation of the electric current density), adopting previously published techniques. 4,8,9,23 Tetrahedral meshes are used in our calculations. The number of tetrahedral elements varies between 16 570 (single-cell) and 19 320 (3-cell configuration).…”

mentioning

confidence: 99%

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Assessing the electro-deformation and electro-poration of biological cells using a three-dimensional finite element model

Shamoon

Dermol–Černe

Rems

et al. 2019

Applied Physics Letters

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In this Letter, we explore how cell electro-deformation and electro-poration are connected. We build a time-domain model of layered concentric shells (a model of biological cells) including their dielectric and elastic properties. We simulate delivery of one trapezoidal voltage pulse to either a single spherical cell or an assembly of three neighboring cells in a specific configuration and calculate cell deformation and pore formation. We describe the qualitative features of the electric field, surface charge density, transmembrane voltage, cell elongation, and pore density distribution at specific times i.e., before, during and after the application of the electric pulse and explore the correlations between them. Our results show that (1) the polarization charge redistribution plays a significant role in the spatial distribution of electrical stresses at μs time scales and (2) the cell deformation and pore density can be correlated with regions of high surface charge density. In future work, our model could be used for understanding basic mechanisms of electro-deformation and electro-poration with high-frequency short bipolar pulses of biological cells in suspension or tissues.

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A Comprehensive Review on Barium Titanate Nanoparticles as a Persuasive Piezoelectric Material for Biomedical Applications: Prospects and Challenges

Sood

Desseigne

Dev

et al. 2022

Small

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Stimulation of cells with electrical cues is an imperative approach to interact with biological systems and has been exploited in clinical practices over a wide range of pathological ailments. This bioelectric interface has been extensively explored with the help of piezoelectric materials, leading to remarkable advancement in the past two decades. Among other members of this fraternity, colloidal perovskite barium titanate (BaTiO3) has gained substantial interest due to its noteworthy properties which includes high dielectric constant and excellent ferroelectric properties along with acceptable biocompatibility. Significant progression is witnessed for BaTiO3 nanoparticles (BaTiO3 NPs) as potent candidates for biomedical applications and in wearable bioelectronics, making them a promising personal healthcare platform. The current review highlights the nanostructured piezoelectric bio interface of BaTiO3 NPs in applications comprising drug delivery, tissue engineering, bioimaging, bioelectronics, and wearable devices. Particular attention has been dedicated toward the fabrication routes of BaTiO3 NPs along with different approaches for its surface modifications. This review offers a comprehensive discussion on the utility of BaTiO3 NPs as active devices rather than passive structural unit behaving as carriers for biomolecules. The employment of BaTiO3 NPs presents new scenarios and opportunity in the vast field of nanomedicines for biomedical applications.

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Cell membrane electroporation modeling: A multiphysics approach

Cited by 52 publications

References 42 publications

Exposure to high-frequency electromagnetic field triggers rapid uptake of large nanosphere clusters by pheochromocytoma cells

Exposure to high-frequency electromagnetic field triggers rapid uptake of large nanosphere clusters by pheochromocytoma cells

Assessing the electro-deformation and electro-poration of biological cells using a three-dimensional finite element model

A Comprehensive Review on Barium Titanate Nanoparticles as a Persuasive Piezoelectric Material for Biomedical Applications: Prospects and Challenges

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