Cell–Microsphere Constructs Formed with Human Adipose‐Derived Stem Cells and Gelatin Microspheres Promotes Stemness, Differentiation, and Controlled Pro‐Angiogenic Potential

Kodali, Anjaneyulu; Lim, Thiam Chye; Leong, David Tai; Tong, Yen Wah

doi:10.1002/mabi.201400094

Cited by 24 publications

(19 citation statements)

References 67 publications

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“…Recently, much attention has been paid to microcarriers composed of biodegradable polymers, such as poly(lactide) (PLA), poly(glicolide) (PGA), and their copolymer poly(lactide‐co‐glycolide) (PLGA), since the polymers possess good mechanical properties, low immunogenicity and toxicity, and an adjustable degradation rate . The biodegradable microcarriers not only provide a large number of cells for the field of cell therapy but also can be directly applied on tissue and organ as microcarriers/cells compound without enzyme treatment and harvest .…”

Section: Introductionmentioning

confidence: 99%

Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation

et al. 2015

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In this study, insulin-like growth factor 1 (IGF-1) was successfully immobilized on the poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA microcarriers via polydopamine (pDA). The results demonstrated that the pDA layer facilitated simple and highly efficient immobilization of peptides on the microcarriers within 20 min. Mouse adipose-derived stem cells (ADSCs) attachment and proliferation on IGF-1-immobilized microcarriers were much higher than non-immobilized ones. More importantly, the IGF-1-immobilized PLGA/HA microcarriers significantly increased alkaline phosphatase (ALP) activity and expression of osteogenesis-related genes of ADSCs. Therefore, it is considered that the IGF-1-decorated PLGA/HA microcarriers will be of great value in the bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation

et al. 2015

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“…Human adipose-derived stem cells (hASCs) have been widely used in cell therapy and tissue regeneration applications due to the multi-potent and effective inducement into a variety of cell types 20 . Bio-artificial liver hepatogenic differentiation of hASCs has attracted much recent research attention.…”

Section: Resultsmentioning

confidence: 99%

A novel cell-printing method and its application to hepatogenic differentiation of human adipose stem cell-embedded mesh structures

Ahn

Lee

et al. 2015

Sci Rep

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We report a cell-dispensing technique, using a core-shell nozzle and an absorbent dispensing stage to form cell-embedded struts. In the shell of the nozzle, a cross-linking agent flowed continuously onto the surface of the dispensed bioink in the core nozzle, so that the bioink struts were rapidly gelled, and any remnant cross-linking solution during the process was rapidly absorbed into the working stage, resulting in high cell-viability in the bioink strut and stable formation of a threedimensional mesh structure. The cell-printing conditions were optimized by manipulating the process conditions to obtain high mechanical stability and high cell viability. The cell density was 1 × 10 7 mL −1 , which was achieved using a 3-wt% solution of alginate in phosphate-buffered saline, a mass fraction of 1.2 wt% of CaCl 2 flowing in the shell nozzle with a fixed flow rate of 0.08 mL min −1 , and a translation velocity of the printing nozzle of 10 mm s −1 . To demonstrate the applicability of the technique, preosteoblasts and human adipose stem cells (hASCs) were used to obtain cell-laden structures with multi-layer porous mesh structures. The fabricated cell-laden mesh structures exhibited reasonable initial cell viabilities for preosteoblasts (93%) and hASCs (92%), and hepatogenic differentiation of hASC was successfully achieved.Tissue engineering has been widely applied to the regeneration of damaged tissues and organs using a combination of cells, an engineered extracellular matrix (or scaffold), and appropriate bioactive growth and differentiation factors 1,2,3 . The scaffold has been shown to be an important factor in cell attachment, growth, and differentiation; however, the mechanisms for the effects of the chemical and biological compositions and the physical structures that are required to encourage proper tissue regeneration are not completely understood 4 .Biomedical scaffolds for tissue engineering should possess various chemical and physical properties, including biocompatibility, with minimal cytotoxic effects to allow high cell attachment and proliferation; should be biodegradable; should have a highly porous structure (appropriate pore size, tortuosity, pore-interconnectivity) to enable easy vascularization and efficient transportation of nutrients and metabolic waste; and should have appropriate mechanical properties to endure the compressive and shear stresses from the micro-environmental conditions 5,6,7 .

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“…Rather than using the microgel suspension as a sacrificial printing medium, here we recognized the potential for a new class of spatially addressable extracellular matrix, in which cellular activity may be dictated by the properties of the suspension. The Segura group and others have explored these types of granular gels as a cell seeded scaffold to capture the benefits the porous nature of the scaffolds provides [34][35][36] , thereby demonstrating the potential for cells to be integrated with microgels. In an interesting twist to the composition of the yield stress fluid for printing, Lewis and colleagues demonstrated freeform vascular printing in a suspension of pure cell organoids 23 .…”

Section: Discussionmentioning

confidence: 99%

Freeform printing of heterotypic tumor models within cell-laden microgel matrices

Molley

Jalandhra

Nemec

et al. 2020

Preprint

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The tissue microenvironment is comprised of a complex assortment of multiple cell types, matrices, membranes and vessel structures. Emulating this complex and often hierarchical organization in vitro has proved a considerable challenge, typically involving segregation of different cell types using layer-by-layer printing or lithographically patterned microfluidic devices. Bioprinting in granular materials is a new methodology with tremendous potential for tissue fabrication. Here, we demonstrate the first example of a complex tumor microenvironment that combines direct writing of tumor aggregates, freeform vasculature channels, and a tunable macroporous matrix as a model to studying metastatic signaling. Our photocrosslinkable microgel suspensions yield local stiffness gradients between particles and the intervening space, while enabling the integration of virtually any cell type. Using computational fluid dynamics, we show that removal of a sacrificial Pluronic ink defines vessel-mimetic channel architectures for endothelial cell linings. Pairing this vasculature with 3D printing of melanoma aggregates, we find that tumor cells within proximity migrated into the prototype vasculature. Together, the integration of perfusable channels with multiple spatially defined cell types provides new avenues for modelling development and disease, with scope for fundamental research and drug development.

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Cell–Microsphere Constructs Formed with Human Adipose‐Derived Stem Cells and Gelatin Microspheres Promotes Stemness, Differentiation, and Controlled Pro‐Angiogenic Potential

Cited by 24 publications

References 67 publications

Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation

Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation

A novel cell-printing method and its application to hepatogenic differentiation of human adipose stem cell-embedded mesh structures

Freeform printing of heterotypic tumor models within cell-laden microgel matrices

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