Cell migration on microposts with surface coating and confinement

Hui, Jianan; Pang, S. W.

doi:10.1042/bsr20181596

Cited by 14 publications

(13 citation statements)

References 38 publications

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“…Topographical guiding patterns. Osteoblast cell migration has been the primary focus of many topographical guiding effect studies 13,[23][24][25] . In this paper, three aspects of surface topography were used to investigate the impact of different guiding patterns on MC3T3-E1 osteoblast cell migration.…”

Section: Resultsmentioning

confidence: 99%

Effects of topographical guidance cues on osteoblast cell migration

Refaaq

Chen

Pang

2020

Sci Rep

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Cell migration is a fundamental process that is crucial for many biological functions in the body such as immune responses and tissue regeneration. Dysregulation of this process is associated with cancer metastasis. In this study, polydimethylsiloxane platforms with various topographical features were engineered to explore the influence of guiding patterns on MC3T3-E1 osteoblast cell migration. Focusing on the guiding effects of grating patterns, variations such as etch depth, pattern discontinuity, and bending angles were investigated. In all experiments, MC3T3-E1 cells on patterned surfaces demonstrated a higher migration speed and alignment when compared to flat surfaces. The study revealed that an increase in etch depth from 150 nm to 4.5 μm enhanced cell alignment and elongation along the grating patterns. In the presence of discontinuous elements, cell migration speed was accelerated when compared to gratings of the same etch depth. These results indicated that cell directionality preference was influenced by a high level of pattern discontinuity. On patterns with bends, cells were more inclined to reverse on 45° bends, with 69% of cells reversing at least once, compared to 54% on 135° bends. These results are attributed to cell morphology and motility mechanisms that are associated with surface topography, where actin filament structures such as filopodia and lamellipodia are essential in sensing the surrounding environment and controlling cell displacement. Knowledge of geometric guidance cues could provide a better understanding on how cell migration is influenced by extracellular matrix topography in vivo.

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Section: Resultsmentioning

confidence: 99%

Effects of topographical guidance cues on osteoblast cell migration

Refaaq

Chen

Pang

2020

Sci Rep

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“…Therefore, NP460 cells migrated with better alignment to the grating orientation of the top layer on platforms that were fully coated with FN. It is suggested that the NP460 cells became more sensitive to the topographical changes on the platforms with FN coating, perhaps related to the improved cell-surface interaction by enhancing the biocompatibility and hydrophilicity of the PDMS substrate [54]. Taken together, this study demonstrates that this two-layer scaffold platform can be used to access the changes in EMT on cell invasiveness that is otherwise only detectable by the use of specific biomarkers.…”

Section: Fibronectin Coating Enhanced Traversing Behavior Of Tgf-β1-tmentioning

confidence: 74%

Using Biomimetic Scaffold Platform to Detect Growth Factor Induced Changes in Migration Dynamics of Nasopharyngeal Epithelial Cells

Lam

Pang

2020

IEEE Access

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A polydimethylsiloxane two-layer scaffold platform was designed to provide a threedimensional biomimetic microsystem that allows the detection of epithelial-to-mesenchymal transition without the use of specific biomarkers. As a proof of concept, a novel microsystem that consisted of two layers of 15 μm thick grating structures was developed. These layers had gratings with 40 μm wide ridges and 10 μm wide trenches, and they were stacked together to form a scaffold platform. To investigate the feasibility of using the engineered platforms for detecting changes in epithelial-to-mesenchymal transition, transforming growth factor beta-1 was added to an untransformed nasopharyngeal epithelial cell line. On flat polydimethylsiloxane surfaces, transforming growth factor beta-1 did not significantly affect nasopharyngeal epithelial size, migration speed, or directionality. However, the effect of transforming growth factor beta-1 treatment on migration speed of nasopharyngeal epithelial cells cultured on the twolayer scaffold platform was significantly different. Furthermore, while almost no untreated nasopharyngeal epithelial cells could squeeze into the 10 μm wide trenches, 21% of the transforming growth factor beta-1 treated nasopharyngeal epithelial cells exhibited traversing behaviors on the two-layer scaffold platforms. Moreover, fibronectin coating on the trenches and bottom layers of the scaffold platforms further enhanced the transforming growth factor beta-1-induced traversing of nasopharyngeal epithelial cells into the narrow trenches. These results demonstrate that the engineered two-layer scaffold microsystem can be used to monitor epithelial-to-mesenchymal transition induced changes in cell migration and invasiveness, paving the way of using these platforms in high throughput drug screening INDEX TERMS 3D scaffold platform, cell migration, epithelial-to-mesenchymal transition, nasopharyngeal cell, traversing into narrow trenches This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.

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“…Similarly, cells encounter complex microenvironments during 3D cell migration. Cells were studied on microposts with different surface coatings and post spacings to mimic physical confinement . Cells traveled faster on the tops of microposts, while cells squeezing between microposts were slower.…”

Section: Deformable Structuresmentioning

confidence: 99%

“…To further investigate how cells respond to a confined space, they were covered with a second set of microposts. When the spacing between the top and bottom microposts was reduced such that cells could adhere to both sets of microposts, it was found that they migrated at a higher speed . In another study, cell migration toward a chemotactic stimulus was observed inside a microfluidic device consisting of microposts embedded in channels of varying cross‐sectional area .…”

Section: Deformable Structuresmentioning

confidence: 99%

(De)form and Function: Measuring Cellular Forces with Deformable Materials and Deformable Structures

Obenaus

Mollica

Sniadecki

2020

Adv Healthcare Materials

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The ability for biological cells to produce mechanical forces is important for the development, function, and homeostasis of tissue. The measurement of cellular forces is not a straightforward task because individual cells are microscopic in size and the forces they produce are at the nanonewton scale. Consequently, studies in cell mechanics rely on advanced biomaterials or flexible structures that permit one to infer these forces by the deformation they impart on the material or structure. Herein, the scientific progression on the use of deformable materials and deformable structures to measure cellular forces are reviewed. The findings and insights made possible with these approaches in the field of cell mechanics are summarized.

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Cell migration on microposts with surface coating and confinement

Cited by 14 publications

References 38 publications

Effects of topographical guidance cues on osteoblast cell migration

Effects of topographical guidance cues on osteoblast cell migration

Using Biomimetic Scaffold Platform to Detect Growth Factor Induced Changes in Migration Dynamics of Nasopharyngeal Epithelial Cells

(De)form and Function: Measuring Cellular Forces with Deformable Materials and Deformable Structures

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