Cell migrations during morphogenesis: Some clues from the slug of <i>Dictyostelium discoideum</i>

Williams, Keith L.; Vardy, Phil H.; Segel, Lee A.

doi:10.1002/bies.950050403

Cited by 28 publications

(18 citation statements)

References 11 publications

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“…We also showed that the observed collective coordinated motion of cells in Dictyostelium, from aggregation to slug movements, follows directly from the behavior of individual cells; no additional assumptions are necessary, such as the squeeze-pull mechanism [49] for the slug. Also since we balance all the forces, the model allows us to study the differences in movements when the cells are dragging themselves forward by grabbing onto other cells and not propelling themselves forward by some internal "jet engine" [45].…”

Section: Discussionmentioning

confidence: 73%

A three-dimensional model of cell movement in multicellular systems

Pálsson

2001

Future Generation Computer Systems

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Section: Discussionmentioning

confidence: 73%

A three-dimensional model of cell movement in multicellular systems

Pálsson

2001

Future Generation Computer Systems

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“…We have shown that the observed collective, coordinated motion of cells in Dd, from aggregation to slug movements, follows directly from the behavior of individual cells; no additional assumptions or mechanisms, such as the squeeze-pull mechanism (49), are necessary to produce motion of an aggregate. We are able to simulate large numbers of interacting cells, accounting for both the passive interactions caused by adhesion and the active locomotory forces, and we allow a restricted class of deformations of individual cells.…”

Section: Discussionmentioning

confidence: 91%

A model for individual and collective cell movement in Dictyostelium discoideum

Pálsson

Othmer

2000

Proc. Natl. Acad. Sci. U.S.A.

260

223

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“…Our results indicate that all cells in the prespore zone move forward at similar speed, while cells in the tip rotate around the slug axis. The squeeze-pull model (16) postulates that the cells in the slug move forward by contraction of a ring of cells in the back of the slug and pulling of cells which are stationary to the substratum in the front of the slug. We never observed stationary cells in the anterior part of the slug as claimed by the squeeze-pull model.…”

Section: Resultsmentioning

confidence: 99%

Three-dimensional scroll waves organize Dictyostelium slugs.

Siegert¹,

Weijer²

1992

Proc. Natl. Acad. Sci. U.S.A.

191

131

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To test the hypothesis that periodic signals and chemotaxis direct later morphogenesis in Dictyostelium discoideum, we investigated cell behavior and cell movement in slugs. Trails of neutral red-stained prestalk and anterior-like cells were recorded by high-resolution digital image processing. Neutral red-stained anterior-like cells in the prespore zone of slugs move straight forward in the direction of slug migration and, furthermore, show coherent periodic cell movement. In contrast, cells in the prestalk zone move along completely different trajectories. Prestalk cells move perpendicular to the direction of slug migration; that is, they rotate around the tip. The cell movement data show that the chemotactic signal in the slug propagates as a three-dimensional scroll wave in the prestalk zone and as a planar wave in the prespore zone. The different behavior of prestalk and prespore cells is most likely caused by a difference in the oscillatory properties of the two cell types. We provide evidence that the slug stage of Dictyostelium behaves like an excitable system and that a (twisted) scroll wave organizes slug formation and migration.The cellular slime mold Dictyostelium discoideum is well suited to the study of cellular communication and the role of biological oscillations in spatiotemporal pattern formation. During the life cycle, solitary amoebae collect to form a multicellular organism. This aggregation process is well characterized and occurs by chemotaxis to periodic cAMP signals initiated by the aggregation center (1). The cAMP pulses, generated and secreted by cells in the aggregation center, are detected and amplified by the monolayer of surrounding cells. This leads to the outward propagation of cAMP waves. The signal propagates as expanding concentric rings or, more often, as spirals (2). These waves direct the cells toward the aggregation center via chemotaxis toward increasing cAMP concentrations. Signal propagation can be detected by direct autoradiographic determination of the spatial variation in cAMP concentration (3) or as an optical density wave caused by periodic cell shape changes associated with chemotactic cell movement (4, 5). After 20-30 waves, cells collect in bifurcating aggregation streams, in which optical density waves are no longer visible. Therefore little is known about signal propagation during multicellular development. Up to 105 cells collect into a compact aggregate (mound),where they begin to differentiate into three major cell types, prestalk, prespore, and anterior-like cells. The cells differentiate in random positions and then undergo chemotactic cell sorting (6, 7). The prestalk cells collect on top of the mound and form a distinct morphological structure (the tip).As soon as a tip is formed, the mound elongates and forms the slug, which then falls over and starts to migrate. The foremost 20% of the slug including the tip consists of prestalk cells, while the remainder is formed by prespore cells. Anterior-like cells are found scattered throughout the presp...

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Cell migrations during morphogenesis: Some clues from the slug of Dictyostelium discoideum

Cited by 28 publications

References 11 publications

A three-dimensional model of cell movement in multicellular systems

A three-dimensional model of cell movement in multicellular systems

A model for individual and collective cell movement in Dictyostelium discoideum

Three-dimensional scroll waves organize Dictyostelium slugs.

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