Cell Motility on Polyethylene Glycol Block Copolymers Correlates to Fibronectin Surface Adsorption

Röttgermann, Peter J. F.; Hertrich, Samira; Berts, Ida; Albert, Max; Segerer, Felix J.; Moulin, Jean‐François; Nickel, Bert; Rädler, Joachim O.

doi:10.1002/mabi.201400246

Cited by 6 publications

(5 citation statements)

References 60 publications

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“…As expected, fibronectin adsorption was decreased by the presence of the PEG coating, as measured by the reduction in the XPS N 1s signal (Table 3); however, protein adsorption was not totally blocked, and all conditions showed nitrogen values ranging from~2% to 5%. These results are in agreement with the studies of Altankov et al and Röttgermann et al [58,59], which showed the capability of PEG coatings to adsorb, to some extent, fibronectin, and facilitate a certain degree of cell adhesion, making the coatings viable for tissue integration applications, such as dental implants. As such, cell adhesion studies with HFFs showed that PEGylated surfaces were still able to support moderate to good levels of both cell attachment and spreading (Figure 4).…”

Section: Discussionsupporting

confidence: 92%

Polyethylene Glycol Pulsed Electrodeposition for the Development of Antifouling Coatings on Titanium

et al. 2020

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Titanium dental implants are widely used for the replacement of damaged teeth. However, bacterial infections at the interface between soft tissues and the implant can impair the functionality of the device and lead to failure. In this work, the preparation of an antifouling coating of polyethylene glycol (PEG) on titanium by pulsed electrodeposition was investigated in order to reduce Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) adhesion while maintaining human fibroblast adhesion. Different pulsed conditions were prepared and characterized by contact angle, Focused Ion Beam (FIB), Fourier Transformed Infrared Spectroscopy in the Attenuated Total Reflectance mode (ATR-FTIR), and X-ray photoelectron spectroscopy (XPS). XPS tested fibronectin adsorption. S. aureus, E. coli and human fibroblast adhesion was tested in vitro in both mono and co-culture settings. Physicochemical characterization proved useful for confirming the presence of PEG and evaluating the efficiency of the coating methods. Fibronectin adsorption decreased for all of the conditions, but an adsorption of 20% when compared to titanium was maintained, which supported fibroblast adhesion on the surfaces. In contrast, S. aureus and E. coli attachment on coated surfaces decreased up to 90% vs. control titanium. Co-culture studies with the two bacterial strains and human fibroblasts showed the efficacy of the coatings to allow for eukaryotic cell adhesion, even in the presence of pre-adhered bacteria.

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Section: Discussionsupporting

confidence: 92%

Polyethylene Glycol Pulsed Electrodeposition for the Development of Antifouling Coatings on Titanium

et al. 2020

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“…Focal adhesions stimulate actin polymerization in lamellipodia and are therefore needed to form protrusions and maintain cell polarization4. Thus, the small number of adhesion points provided on PEG areas54 can explain the switch from run to rest state at the fibronectin/PEG interface.…”

Section: Resultsmentioning

confidence: 99%

Ring-Shaped Microlanes and Chemical Barriers as a Platform for Probing Single-Cell Migration

Schreiber

Segerer

Wagner

et al. 2016

Sci Rep

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Quantification and discrimination of pharmaceutical and disease-related effects on cell migration requires detailed characterization of single-cell motility. In this context, micropatterned substrates that constrain cells within defined geometries facilitate quantitative readout of locomotion. Here, we study quasi-one-dimensional cell migration in ring-shaped microlanes. We observe bimodal behavior in form of alternating states of directional migration (run state) and reorientation (rest state). Both states show exponential lifetime distributions with characteristic persistence times, which, together with the cell velocity in the run state, provide a set of parameters that succinctly describe cell motion. By introducing PEGylated barriers of different widths into the lane, we extend this description by quantifying the effects of abrupt changes in substrate chemistry on migrating cells. The transit probability decreases exponentially as a function of barrier width, thus specifying a characteristic penetration depth of the leading lamellipodia. Applying this fingerprint-like characterization of cell motion, we compare different cell lines, and demonstrate that the cancer drug candidate salinomycin affects transit probability and resting time, but not run time or run velocity. Hence, the presented assay allows to assess multiple migration-related parameters, permits detailed characterization of cell motility, and has potential applications in cell biology and advanced drug screening.Migrating cells play a pivotal role in morphogenesis 1 , immune responses 2 , and cancer metastasis 3 . Their style of motion, often assigned as crawling, is powered by complex cytoskeletal rearrangements that deform and propel the cell. On solid surfaces, eukaryotic cells extend protrusions, which attach to the substrate and are then actively retracted, thus dragging the cell forward. The formation of the leading protrusion of a migrating cell, the lamellipodium, is driven by actin polymerization, while adhesion and contraction are predominantly regulated by integrin-based focal adhesions and the actomyosin apparatus 4,5 . Coupling of focal adhesion complexes to the cytoskeletal network in turn reinforces actin assembly and hence lamellipodia extension 6 . The complex interplay between actomyosin contractility and focal adhesions, which are capable of sensing and transducing chemical and mechanical cues in the extracellular environment, renders the cell sensitive to external stimuli such as the composition and rigidity of the extracellular matrix (ECM) and the underlying substrate 7,8 .In recent studies, various theoretical models for cell migration have been proposed and implemented. These implementations range from molecular level approaches, which describe cell migration in terms of internal reaction diffusion dynamics 9-11 to coarse grained approaches in which individual cells are resembled by sets of pixels 12-14 or interacting, self-propelled geometrical objects [15][16][17] . Many of these models are able to reproduce the basic ...

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“…This single cell platform allows events in hundreds of cells to be followed in parallel. Single cell arrays are obtained via micro-patterned structured surfaces, upon which cells self-organise into an ordered array (Röttgermann et al 2014a(Röttgermann et al , 2014b. In short, polydimethylsiloxane (PDMS) stamps were placed in 8well slides (ibidi GmbH, Martinsried, Germany) as a mask and selectively treated with oxygen plasma, 40 W for 3 min (Femto Diener, Ebhausen, Germany).…”

Section: Ht/c Assays At Lmumentioning

confidence: 99%

Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines

et al. 2017

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Manufactured nanomaterials (MNMs) selected from a library of over 120 different MNMs with varied compositions, sizes, and surface coatings were tested by four different laboratories for toxicity by high-throughput/-content (HT/C) techniques. The selected particles comprise 14 MNMs composed of CeO, Ag, TiO, ZnO and SiO with different coatings and surface characteristics at varying concentrations. The MNMs were tested in different mammalian cell lines at concentrations between 0.5 and 250 µg/mL to link physical-chemical properties to multiple adverse effects. The cell lines are derived from relevant organs such as liver, lung, colon and the immune system. Endpoints such as viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential, lysosomal acidification and steatosis have been studied. Soluble MNMs, Ag and ZnO, were toxic in all cell types. TiO and SiO MNMs also triggered toxicity in some, but not all, cell types and the cell type-specific effects were influenced by the specific coating and surface modification. CeO MNMs were nearly ineffective in our test systems. Differentiated liver cells appear to be most sensitive to MNMs, Whereas most of the investigated MNMs showed no acute toxicity, it became clear that some show adverse effects dependent on the assay and cell line. Hence, it is advised that future nanosafety studies utilise a multi-parametric approach such as HT/C screening to avoid missing signs of toxicity. Furthermore, some of the cell type-specific effects should be followed up in more detail and might also provide an incentive to address potential adverse effects in vivo in the relevant organ.

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Cell Motility on Polyethylene Glycol Block Copolymers Correlates to Fibronectin Surface Adsorption

Cited by 6 publications

References 60 publications

Polyethylene Glycol Pulsed Electrodeposition for the Development of Antifouling Coatings on Titanium

Polyethylene Glycol Pulsed Electrodeposition for the Development of Antifouling Coatings on Titanium

Ring-Shaped Microlanes and Chemical Barriers as a Platform for Probing Single-Cell Migration

Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines

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