Cell orientation response to cyclically deformed substrates: Experimental validation of a cell model

Wang, Huicong; Ip, Wallace; Boissy, Raymond E.; Grood, Edward S.

doi:10.1016/0021-9290(95)00101-8

Cited by 142 publications

(167 citation statements)

References 21 publications

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“…4, with the global substrate strain increasing, three regimes can be found and are divided by two threshold strains: (i) when the global substrate strain is below the first threshold level, the contact central angle almost keeps unchanged; (ii) after the first threshold level, the contact central angle decreases very quickly with an increasing substrate strain; (iii) when the global substrate strain exceeds the second threshold level, the contact central angle almost equals zero. All the phenomena are very similar to the findings in the experiment of cells cultured on a cyclically stretch substrate, where cell reorientation is controlled by two threshold stretch amplitudes (Dartsch and Hammerle, 1986;Neidlingerwilke et al, 1994;Wang et al, 1995), and are consistent well with the results of an earlier model of an elastic cylinder adhering on a stretched substrate (Chen and Gao, 2006b).…”

Section: Resultssupporting

confidence: 88%

“…However, the behaviors of the contact area predicted by the present model show several features which appear to be qualitatively similar to that of cells cultured on a cyclically stretched substrate. Experiments on cell reorientation in response to cyclic substrate strain also show three characteristic regimes with two threshold strain amplitudes (Dartsch and Hammerle, 1986;Neidlingerwilke et al, 1994;Wang et al, 1995). It was found that cells do not respond to strain amplitudes smaller than 1-2% (Dartsch and Hammerle, 1986).…”

Section: Resultsmentioning

confidence: 99%

“…Another set of experiments have shown that cells cultured on a cyclically stretched substrate tend to reorient themselves away from the stretch direction (Buck, 1980;Dartsch and Hammerle, 1986;Jungbauer et al, 2008;Moretti et al, 2004;Neidlingerwilke et al, 1994;Wang et al, 1995). Dartsch and Hammerle (1986) further noted that cells did not respond to small stretch amplitudes (less than 2%), suggesting that there exists a threshold stretch amplitude to initiate cell reorientation.…”

Section: Introductionmentioning

confidence: 99%

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Adhesion of a gas-filled membrane on a stretched substrate

Chen

2015

International Journal of Solids and Structures

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a b s t r a c tA two-dimensionally adhesive contact model is established, in which a gas-filled elastic membrane adheres on a stretched substrate. The free energy of the system is achieved, minimization of which leads to the relationship between the contact width and the global substrate strain. The contact solution exhibits three distinct regimes characterized by two threshold strains: (i) the contact size is hardly affected by the external loading acted on the substrate when the global substrate strain is below the first threshold value; (ii) the size of the contact is reduced quickly as the force is between the two threshold levels; (iii) the contact size tends to vanish when the global substrate strain exceeds the second threshold level. All the results share a number of common features with the experimental observation of cell orientation on a stretched substrate. Effects of the internal pressure, the tensile stiffness of the membrane and the interface work of adhesion on the two threshold levels are further discussed. The finding in the present paper should be helpful for deep understanding of adhesion mediated deformation sensing mechanism by which cells can detect mechanical signals in extracellular matrices and the design of adhesion mediated deformation sensors.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Adhesion of a gas-filled membrane on a stretched substrate

Chen

2015

International Journal of Solids and Structures

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“…While this loading protocol was not intended to represent a particular pathologic or surgical scenario, there are realistic examples of altered loading conditions that may give rise to comparable levels of tissue remodeling. For instance, a similar transition in mechanical environment could arise due to surgical implantation of a statically cultured vascular graft or myocardial patch into the dynamic host tissue, since most cell types align parallel to a static mechanical stretch but perpendicular to cyclic stretching 8,30,34 . Nevertheless, the relevant message from this work is that when designing living tissues, one cannot necessarily rely on contact guidance cues to sustain structural and functional anisotropy.…”

Section: Discussionmentioning

confidence: 99%

Remodeling of Engineered Tissue Anisotropy in Response to Altered Loading Conditions

2008

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Structural and mechanical anisotropy are critical to the function of many engineered tissues. This study examined the ability of anisotropic tissue constructs to overcome contact guidance cues and remodel in response to altered mechanical loading conditions. Square tissues engineered from dermal fibroblasts and type-I collagen were uniaxially loaded to induce cell and matrix alignment. After an initial time, t*, of 5 to 72 hrs, loading was switched from the x-axis to the y-axis. Cell alignment was examined throughout the experiment until a steady state was reached. Before t*, cells spontaneously aligned in the x-direction. After t*, the strength of alignment transiently decreased then increased, and mean cell orientation transitioned from the x-to the y-direction following an exponential time course with a time constant that increased with t*. Collagen fiber orientation exhibited similar trends that could not be explained by passive kinematics alone. Structural realignment resulted in concomitant changes in biaxial tissue mechanical properties. The findings suggest that even highly aligned engineered tissue constructs retain the capacity to remodel in response to altered mechanical stimuli. This may have important functional consequences when an anisotropic engineered tissue designed in vitro is surgically implanted into a mechanically complex graft site.

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“…Cells are known to respond to mechanical forces exerted through surrounding fluid, adhering beads or substrates [9,[12][13][14], and they could detach, slip or roll on a substrate in response to these forces [15][16][17][18][19][20][21][22]. For example, cells on a cyclically stretched substrate tend to reorient themselves away from the stretching direction [23][24][25][26][27], and cells migrate along a substrate with rigidity gradient (durotaxis) [18]. Blood cells are found to undergo a transition from rolling to translational motion on a blood vessel wall under increasing hydrodynamic shear forces [19], exemplifying a general fact that it takes less effort for a round object to roll than to slip on a substrate [28,29].…”

Section: Introductionmentioning

confidence: 99%