Cell origin and differentiation in the repair of full-thickness defects of articular cartilage.

Shapiro, Frederic; Koide, S; Glimcher, Melvin J.

doi:10.2106/00004623-199304000-00009

Cited by 1,148 publications

(987 citation statements)

References 9 publications

Supporting

Mentioning

914

Contrasting

Unclassified

Order By: Relevance

“…The molecular and histochemical responses of the repair tissue in this study were slightly delayed compared to previous studies investigating repair tissue in rabbit models of full-thickness cartilage defects [12,25,28,33]. In this study, matrix metachromasia and procollagen type I1 in situ hybridization reactions, both cellular markers of intrinsic healing attempts, were not detected until week 4, compared to evidence of early chondrogenesis at 2 weeks in rabbits.…”

Section: Discussioncontrasting

confidence: 63%

“…Insulin-like growth factor-I (IGF-I) is one of the more important of these anabolic peptides. It has been shown in vitro to enhance cartilage matrix metabolism, both through increased synthesis of aggrecan, hyaluronan, and link protein, and through the inhibition of proteoglycan degradation, culminating in maintenance of a net positive proteoglycan balance [6,17,33,36,371. In addition, IGF-I aids in maintenance of the mechanical properties of cartilage, including the equilibrium modulus and electrokinetic coefficient [32], and it protects cartilage from the matrix degradative events following exposure to interleukin-1 and/or tumor necrosis factor-ct [11,37].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Insulin‐like growth factor‐I gene expression patterns during spontaneous repair of acute articular cartilage injury

Fortier

Balkman

Sandell

et al. 2001

Journal Orthopaedic Research

View full text Add to dashboard Cite

This study evaluated the constitutive insulin-like growth factor-I (IGF-I) gene expression pattern in spontaneously healing cartilage defects over the course of 16 weeks, and correlated the tissue morphology and matrix gene expression with IGF-I mRNA levels. Full-thickness 15 mm cartilage defects were debrided in the femoral trochlea of both femoropatellar joints of 8 horses and the healing defects examined 2, 4, 8, or 16 weeks after surgery. Samples were harvested for histologic assessment of tissue healing using H&E staining, toluidine blue histochemical reaction for proteoglycan deposition, and in situ hybridization and immunohistochemistry procedures to demonstrate collagen type I1 mRNA and protein expression. Total R N A was isolated for Northern analysis to measure cartilage matrix molecule expression, and for semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) to determine IGF-I gene expression patterns in healing cartilage defects. Full-thickness cartilage defects in horses were slow to heal compared to smaller lesions in similar locations in other animals. However, a progressive decline in tissue cellularity and vascularity, and increased tissue organization were observed on H&E stained specimens over the 16-week experiment. Evidence of early chondrogenic repair was detected through collagen type 11 in situ hybridization and immunohistochemistry. However, levels of collagen type I1 and aggrecan mRNA in lesions were not abundant on Northern analysis indicating incomplete chondrogenesis. IGF-I message expression followed a cyclic pattern with low levels at 2 weeks, followed by an increase at 4 and 8 weeks, and a subsequent decline at 16 weeks. There was no direct correlation between the stage of healing and cartilage matrix message expression, and the abundance of IGF-I mRNA in the healing lesions. In conclusion, this study demonstrated that the spontaneous healing of articular defects was accompanied by a temporal fluctuation in IGF-I gene expression which was discoordinate to the steady rise in expression of cartilage matrix molecules such as procollagen type 11.

show abstract

Section: Discussioncontrasting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Insulin‐like growth factor‐I gene expression patterns during spontaneous repair of acute articular cartilage injury

Fortier

Balkman

Sandell

et al. 2001

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…The success rates with these treatments vary greatly. While some of these approaches show promise, many lead to formation of fibrous tissue, apoptosis, and further cartilage degeneration (1)(2)(3)(4)(5)(6). To address the need for improved therapies to promote cartilage repair or regeneration, a number of novel tissue engineering approaches have been developed for the treatment of cartilage defects or osteoarthritis.…”

mentioning

confidence: 99%

Potent induction of chondrocytic differentiation of human adipose‐derived adult stem cells by bone morphogenetic protein 6

Estes

Guilak

2006

Arthritis & Rheumatism

271

212

View full text Add to dashboard Cite

Objective. Recent studies have identified an abundant source of multipotent progenitor cells in subcutaneous human adipose tissue, termed human adiposederived adult stem cells (ADAS cells). In response to specific media formulations, including transforming growth factor ␤1 (TGF␤1), these cells exhibit significant ability to differentiate into a chondrocyte-like phenotype, expressing cartilage-specific genes and proteins such as aggrecan and type II collagen. However, the influence of other growth factors on the chondrogenic differentiation of ADAS cells is not fully understood. This study was undertaken to investigate the effects of TGF␤1, TGF␤3, insulin-like growth factor 1, bone morphogenetic protein 6 (BMP-6), and dexamethasone, in various combinations, on the chondrogenic potential of ADAS cells in alginate beads.Methods. The chondrogenic response of alginateencapsulated ADAS cells was measured by quantitative polymerase chain reaction, 3 H-proline and 35 S-sulfate incorporation, and immunolabeling for specific extracellular matrix components.Results. Significant differences in chondrogenesis were observed under the different culture conditions for all outcomes measured. Most notably, BMP-6 upregulated AGC1 and COL2A1 expression by an average of 205-fold and 38-fold, respectively, over day-0 controls, while down-regulating COL10A1 expression by ϳ2-fold.Conclusion. These findings suggest that BMP-6 is a potent inducer of chondrogenesis in ADAS cells, in contrast to mesenchymal stem cells, which exhibit increased expression of type X collagen and a hypertrophic phenotype in response to BMP-6. Combinations of growth factors containing BMP-6 may provide a novel means of regulating the differentiation of ADAS cells for applications in the tissue-engineered repair or regeneration of articular cartilage.

show abstract

“…Higher mechanical strength for fixation of the fragments also has been reported [16]. In addition, screws have been formulated to promote recruitment of mesenchymal stem cells from bone marrow to enhance chondral repair [21].…”

Section: Introductionmentioning

confidence: 92%

“…Therefore, cartilage defects are created on the fragments at the entrance holes of the devices. It has been reported small cartilage defects less than 3 mm in diameter are filled with regenerated cartilage when they are created on living or vascularized subchondral bone [21]. However, there have been no reports regarding cartilage repair of detached osteochondral fragments.…”

Section: Introductionmentioning

confidence: 99%

Bone Screws Have Advantages in Repair of Experimental Osteochondral Fragments

Kono

Mori

Uchio

2012

Clinical Orthopaedics &Amp; Related Research

View full text Add to dashboard Cite

Background Cartilage defects are created on intraarticular osteochondral fragments at the entrance holes of fixation devices when these fragments are fixed to the original sites. Conventional fixation devices hinder repair of these defects and there is a latent risk of secondary osteoarthritis. We therefore developed a novel fixation device system consisting of bone screws made of cortical bone for osteochondral fragments to improve repair of these surface defects. Questions/purposes We asked whether bone screws had advantages over poly-L-lactic acid (PLLA) screws in terms of (1) gross assessment of the surface, (2) volume and histologic quality of the repair tissue, and (3) biomechanical assessment of the tissue stiffness.Methods We examined gross morphology, microCT, histology, and stiffness of the repaired tissue with PLLA (n = 32) and bone (n = 32) screws in a rabbit model of osteochondral fracture, compared with normal controls (n = 16). Results Gross morphology and histology revealed better quality with bone screws than with PLLA screws. Mean repaired volumes in microCT were 70.6% ± 14% with bone screws and 50.3% ± 15% with PLLA screws. Average stiffness values for PLLA screws, bone screws, and normal cartilage were 1.67 ± 0.54 N/mm, 2.63 ± 0.42 N/mm, and 3.15 ± 0.49 N/mm, respectively. Conclusions Our results show better repaired tissue was observed for quality and quantity when chondral fractures were treated with bone screws than when treated with PLLA screws. Clinical Relevance Bone screws made of cortical bone may have applications in clinical situations for the fixation of intraarticular osteochondral fragments.

show abstract

Cell origin and differentiation in the repair of full-thickness defects of articular cartilage.

Cited by 1,148 publications

References 9 publications

Insulin‐like growth factor‐I gene expression patterns during spontaneous repair of acute articular cartilage injury

Insulin‐like growth factor‐I gene expression patterns during spontaneous repair of acute articular cartilage injury

Potent induction of chondrocytic differentiation of human adipose‐derived adult stem cells by bone morphogenetic protein 6

Bone Screws Have Advantages in Repair of Experimental Osteochondral Fragments

Contact Info

Product

Resources

About