Cell pellets from dental papillae can reexhibit dental morphogenesis and dentinogenesis

Yu, Jinhua; Shi, Jun; Deng, Zhi Hong; Zhuang, Heng; Nie, Xin; Wang, Ruo Ning; Jin, Yan

doi:10.1016/j.bbrc.2006.05.096

Cited by 43 publications

(45 citation statements)

References 38 publications

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“…3 Dental pulp stem cells (DPSCs) have been differentiated 4 into odontoblasts; 5,6 osteoblasts; 1 adipocytes, chondrocytes, and osteocytes; 7,8 nerve cells; 8 and hepatocytes. 9 Furthermore, the combined use of tissue engineering and DPSCs has enabled the regeneration of a dentin-pulp complex, 10 the generation of functional roots, 11 and even the production of a complete tooth. 12 Dental pulp stem cells have survived and proliferated in a self-assembling peptide hydrogel that could conceptually be used in regenerative endodontics, allowing stem cell therapy inside the root canal.…”

Section: Introductionmentioning

confidence: 99%

Culture of human dental pulp cells at variable times post-tooth extraction

Araújo

Oliveira²,

et al. 2018

Braz. oral res.

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The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.

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Section: Introductionmentioning

confidence: 99%

Culture of human dental pulp cells at variable times post-tooth extraction

Araújo

Oliveira²,

et al. 2018

Braz. oral res.

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“…As a type of odontoblast progenitor cell, dental papilla mesenchymal cells possess the potential to differentiate into odontoblast cells. This type of cell could spontaneously differentiate into odontoblast-like cells under normal in vitro culture conditions [27]. As with the transduction of SV40Tag, the cells showed a dedifferentiation state [28].…”

Section: Discussionmentioning

confidence: 96%

Application of immortalized mouse dental papilla cells for tooth bioengineering

Epasinghe¹,

Kim²,

Wu³

et al. 2018

Dent Oral Craniofac Res

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Mouse dental papilla cells (MDPCs) can be differentiated into component cell types that have the ability to regenerate the pulpo-dentinal complex. At the cap stage of tooth development, MDPC component cells are programmed for tooth generation. Therefore, isolated and cultivated component cells can be useful in evaluating cellular, molecular, and environmental scenarios in tooth germ formation and tooth morphogenesis. Increasing the proliferation capacity of MDPCs and preserving their original phenotypic and genotypic characteristics for tooth organ regeneration are the main focus of this study. An immortalized mouse dental papilla cell line was created via the intracellular insertion of SV40 T antigens into the nucleus by lentivirus particles. The generated clonally isolated SV40 T immortalized MDPC line was then characterized and validated for transfection success and efficiency. These cells displayed a higher proliferation rate, and both genotype and phenotype characteristics were similar to those of the original primary cell line. These results were verified via the expression of a broad array of tooth-specific markers. Furthermore, the test results to show that transformed cells had preserved multi-potency were also positive. Thus, the stable immortalized MDPCs may be used to determine the mechanisms of an array of developmental phenomena, such as early dental cell proliferation, reconstitution of tooth germ, dentine mineralization and other significant growth factor signaling pathways influencing tooth morphogenesis.

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“…Human dental cervical loop epithelial cells (hDCLECs), human dental papilla mesenchymal cells (hDPMCs), human dental follicle cells (hDFCs) were dissected from the lower first deciduous molar tooth germs of 6-month-old human fetal cadaver tissues and cultured. For hDPMCs culture, dental papillae were physically separated from molar germs under stereomicroscope and minced into pieces in 0.01 M phosphate-buffered saline (PBS; Gibco-BRL, Grand Island, USA) and digested with type I collagenase (0.66 mg/mL; Sigma, St. Louis, MO) for 40 min (Yu et al 2006). Single cell suspensions were generated by filtration through a 70-lm strainer, washed with Dulbecco's modified Eagle's medium (DMEM, Gibco-BRL, Grand Island, USA) supplemented with 10% FBS, and then placed into culture flasks and cultured in 5% CO2 at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…The putative progenitor/stem cells residing in dental papillae are endowed with differentiation potentials into odontoblasts lineage under appropriate conditions in vitro (Kikuchi et al 2004). Previously, we have succeed in demonstrating on a persuasive basis that even without the inductive signals from dental epithelial, rat dental papilla mesenchymal cells (DPMCs) of tooth germs at the late bell stage can still fulfill odontoblast differentiation and dentinogenesis, endowing the relatively independent and critical role for dental mesenchymal cells in tooth development and morphogenesis (Yu et al 2006). Putative stem cells obtained from dental papilla tissue of developing molars of human and porcine origin were confirmed as SCAP (Stem Cells from Apical Papilla), which had a greater capacity for dentin regeneration than dental pulp stem cells (Sonoyama et al 2006(Sonoyama et al , 2008.…”

Section: Introductionmentioning

confidence: 96%

“…Our findings demonstrating the expression of ADAM28 in human and murine tooth germ indicated that this protein might impart intrinsic regulation of reciprocal interaction of dental epithelium and mesenchyme. Concerning the highly recognized functionality of dental papilla mesenchymal cells (DPMCs) during tooth development (Yu et al 2006;Yuan et al 2008), it is of paramount importance to disclose the effect ADAM28 on biological behavior of DPMCs.…”

Section: Introductionmentioning

confidence: 99%

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Essential role of ADAM28 in regulating the proliferation and differentiation of human dental papilla mesenchymal cells (hDPMCs)

Zheng

Tang

Deng

et al. 2008

Histochem Cell Biol

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Dental papilla mesenchymal cells (DPMCs) have been supposed to possess the relatively independent and critical role for tooth development and morphogenesis. Here, we characterized the role of ADAM28, a member of a disintegrin and metalloproteinase (ADAM) family, in the regulative mechanisms of odontogenic capability of hDPMCs. Immunofluorescence staining showed the ubiquitous expression of ADAM28 in multiple human dental mesenchymal and epithelial cells. After confirming the effect of eukaryotic expression plasmid containing ADAM28 coding region and ADAM28 antisense oligodeoxynucleotide (AS-ODN), we respectively transfected them into hDPMCs and observed the biological markers for proliferation and differentiation. Overexpression of ADAM28 favored the proliferation and lineage-specific differentiation of hDPMCs, while blockage of ADAM28 exerted the opposite effects and induced apoptosis. These results identified an unrecognized hypothesis that ADAM28 may function as positive regulator of growth and differentiation of hDPMCs and act as an important molecule mediating reciprocal epithelial-mesenchymal signaling during tooth organ development.

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Cell pellets from dental papillae can reexhibit dental morphogenesis and dentinogenesis

Cited by 43 publications

References 38 publications

Culture of human dental pulp cells at variable times post-tooth extraction

Culture of human dental pulp cells at variable times post-tooth extraction

Application of immortalized mouse dental papilla cells for tooth bioengineering

Essential role of ADAM28 in regulating the proliferation and differentiation of human dental papilla mesenchymal cells (hDPMCs)

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