Cell Penetrating Peptides

Langel, eUlo

doi:10.2174/9781681082691116010011

Polarity Index in Proteins - A Bioinformatics Tool 2016

DOI: 10.2174/9781681082691116010011

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Cell Penetrating Peptides

eUlo Langel¹

Abstract: Background: Cell penetrating peptides have gained much recognition as a versatile transport vehicle for the intracellular delivery of wide range of cargoes (i.e. oligonucelotides, small molecules, proteins, etc.), that otherwise lack bioavailability, thus offering great potential as future therapeutics. Keeping in mind the therapeutic importance of these peptides, we have developed in silico methods for the prediction of cell penetrating peptides, which can be used for rapid screening of such peptides prior to… Show more

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Cited by 2 publications

References 22 publications

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A gene delivery method mediated by three arginine-rich cell-penetrating peptides in plant cells

Liu¹,

Chou²,

Lee

2013

asb

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The transient gene delivery systems are generally mediated by viral infection, particle bombardment, electroporation, and microinjection for transferring exogenous DNAs into host cells. Recently, the peptide-mediated DNA delivery system became a novel tool for gene transfer, and these peptides, such as cell-penetrating peptides (CPPs), contained the ability of permeating plasma membranes and carrying cargoes to enter cells. In this study, we use three different arginine-rich CPPs, synthetic nona-arginine (SR9), histidine-rich nona-arginine (HR9), and Pas nona-arginine (PR9), for gene delivery into root-tip cells of mung bean and rice. We demonstrate that these arginine-rich CPPs have the ability to transfer Cyanine 3 (Cy3)-labeled DNAs into plant cells. Moreover, the CPP-mediated DNA transfer in cells was confirmed by polymerase chain reaction (PCR) analysis. Finally, our results of the functional gene assay indicated that DNA exerts its biological activity after intracellular delivery via CPPs. This CPP-mediated gene delivery method is characterized by the simplicity, noninvasion, and efficiency. Three arginine-rich CPPs may be ideal tools for the research of plant gene transformation.

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A gene delivery method mediated by three arginine-rich cell-penetrating peptides in plant cells

Liu¹,

Chou²,

Lee

2013

asb

View full text Add to dashboard Cite

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Strategies toward structural and functional ‘optimization’ of animal peptide toxins

Publications¹,

Andreotti²,

Ziadi³

et al. 2012

Int J Med Biomed Res

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Background: venoms of a variety of animal species (i.e. scorpions, snakes, spiders, sea anemones, marine cone snails, worms, and insects) are rich sources of bioactive compounds that possess obvious pharmacological, therapeutic and/or biotechnological values. A majority of these compounds are peptides that mainly target enzymes, membrane receptors or ion channels. Aim: In recent years, much efforts of researchers have been focused on characterizing and 'improving' the pharmacological profile of some venom toxins, mainly because of their structural/functional diversity and potential value as chemotherapeutic drugs in the treatment of specific human pathologies. Materials and methods: This study reviews the strategies towards optimization of animal peptide toxins. Results: These peptides are most often in a size range that allows their production in vitro by chemical synthesis or genetic engineering. Unfortunately, they rarely display the required characteristics in terms of selectivity, affinity, stability and targeting with regard to the desired application. During the last decade, a number of successful structural approaches or strategies have been developed to improve the intrinsic potential of venom peptides. Using different strategies (reviewed herein), a number of highly potent and selective toxinderived candidate drugs were designed and produced by chemical synthesis. Conclusion: Such strategies proved to be effective as several toxin-based drugs are already marketed while an increasing number of candidate chemotherapeutics are currently being developed in clinical phase.

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Cell Penetrating Peptides

Cited by 2 publications

References 22 publications

A gene delivery method mediated by three arginine-rich cell-penetrating peptides in plant cells

A gene delivery method mediated by three arginine-rich cell-penetrating peptides in plant cells

Strategies toward structural and functional ‘optimization’ of animal peptide toxins

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