Cell-permeable organic fluorescent probes for live-cell long-term super-resolution imaging reveal lysosome-mitochondrion interactions

Han, Yubing; Li, Meihua; Qiu, Fengwu; Zhang, Meng; Zhang, Yuhui

doi:10.1364/pibm.2017.w3a.128

Cited by 19 publications

(29 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To further clarify the resolution of the dye for imaging mitochondria, we obtained a full-width at the half-maximum (FWHM) up to 80 nm under SIM (Fig. 2e-f), which is similar to the Atto 647 mitochondrial dye reported by Han et al (FWHM: 91 nm) 34 . Such results suggest that our dye offers higher resolution and precision for tracking mitochondria under SIM.…”

Section: Resultssupporting

confidence: 73%

“…Results indicated that the dye had uniform tissue permeability at different depths in living cells. The photobleaching properties of the dye directly affected the monitoring of mitochondrial dynamic processes 33, 34 . Next, we performed a long-term continuous laser (165 s) to stimulate cells in order to monitor the photostability of the dye in the same section of the cell (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Mitochondria rank among the most dynamic organelles in cells 34 , and understanding their dynamic processes is important for analyzing the causes of many diseases 37 . Therefore, a strategy for visualizing the dynamics of mitochondrial at the nanoscale level has important implications for understanding mitochondria-related diseases.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore

Chen

Jin²,

Shao

et al. 2018

Preprint

View full text Add to dashboard Cite

Combining luminescent transition metal complex (LTMC) dye with super-resolution 19 microscopy is an excellent strategy for the long-term visualization of the dynamics of subcellular 20 structures in living cells. However, it remains unclear whether the third-row LTMC dye is applicable for 21 All rights reserved. No reuse allowed without permission.was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/312371 doi: bioRxiv preprint first posted online May. suggesting that these two processes are independence. 32All rights reserved. No reuse allowed without permission.was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

show abstract

Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore

Chen

Jin²,

Shao

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…23 To visualize uptake at high resolution in vivo we labeled lysozyme with very bright and photo-stable fluorophores. 24 We found that uptake of lysozyme and albumin occurred almost exclusively in S1 segments. Although it is somewhat intuitive that uptake of filtered molecules would be higher in the first segment immediately after the glomerulus, the magnitude of the S1/S2 differences was surprising.…”

Section: Discussionmentioning

confidence: 70%

“…Commercially available prelabeled forms of albumin and dextran were used. To label lysozyme we used atto dyes, which are extremely bright and highly photo-stable, 24 thus enabling high-resolution imaging in vivo. Experiments with each ligand were repeated with two different labels to exclude a confounding effect of the fluorophores on uptake.…”

Section: Uptake Of Filtered Proteins By Rme Occurs Almost Exclusivelymentioning

confidence: 99%

Combined Structural and Functional Imaging of the Kidney Reveals Major Axial Differences in Proximal Tubule Endocytosis

Schuh¹,

Polesel²,

Platonova³

et al. 2018

JASN

View full text Add to dashboard Cite

Background The kidney proximal convoluted tubule (PCT) reabsorbs filtered macromolecules via receptor-mediated endocytosis (RME) or nonspecific fluid phase endocytosis (FPE); endocytosis is also an entry route for disease-causing toxins. PCT cells express the protein ligand receptor megalin and have a highly developed endolysosomal system (ELS). Two PCT segments (S1 and S2) display subtle differences in cellular ultrastructure; whether these translate into differences in endocytotic function has been unknown.Methods To investigate potential differences in endocytic function in S1 and S2, we quantified ELS protein expression in mouse kidney PCTs using real-time quantitative polymerase chain reaction and immunostaining. We also used multiphoton microscopy to visualize uptake of fluorescently labeled ligands in both living animals and tissue cleared using a modified CLARITY approach.Results Uptake of proteins by RME occurs almost exclusively in S1. In contrast, dextran uptake by FPE takes place in both S1 and S2, suggesting that RME and FPE are discrete processes. Expression of key ELS proteins, but not megalin, showed a bimodal distribution; levels were far higher in S1, where intracellular distribution was also more polarized. Tissue clearing permitted imaging of ligand uptake at singleorganelle resolution in large sections of kidney cortex. Analysis of segmented tubules confirmed that, compared with protein uptake, dextran uptake occurred over a much greater length of the PCT, although individual PCTs show marked heterogeneity in solute uptake length and three-dimensional morphology.Conclusions Striking axial differences in ligand uptake and ELS function exist along the PCT, independent of megalin expression. These differences have important implications for understanding topographic patterns of kidney diseases and the origins of proteinuria.

show abstract

Targeted fluorescent imaging of a novel FITC-labeled PSMA ligand in prostate cancer

et al. 2021

View full text Add to dashboard Cite

In this study, we synthesized a novel fluorescein isothiocyanate (FITC)-labeled prostate-specific membrane antigen (PSMA) ligand (PSMA-FITC) via the Fmoc solid-phase synthesis method, and the application value of PSMA-FITC in targeted fluorescence imaging of PSMA-positive prostate cancer was evaluated. The PSMA ligand developed based on the Glu-urea-Lys structure was linked to FITC by aminocaproic acid (Ahx) to obtain PSMA-FITC. The new probe was evaluated in vitro and in vivo. Fluorescence microscopy examination of PSMA-FITC in PSMA(+) LNCaP cells, PSMA(−) PC3 cells, and blocked LNCaP cells showed that the binding of PSMA-FITC with PSMA was target-specific. For in vivo optical imaging, PSMA-FITC exhibited rapid 22Rv1 tumor targeting within 30 min of injection, and the highest tumor-background ratio (TBR) was observed 60 min after injection. The TBR was 3.45 ± 0.31 in the nonblocking group and 0.44 ± 0.13 in the blocking group, which was consistent with the in vitro results. PSMA-FITC is a promising probe and has important reference value for the development of PSMA fluorescent probes. In the future, it can be applied to obtain accurate tumor images for radical prostatectomy.

show abstract

Cell-permeable organic fluorescent probes for live-cell long-term super-resolution imaging reveal lysosome-mitochondrion interactions

Abstract: Two kinds of cell-permeable organic fluorescent probes with high photostability were developed for lysosomes and mitochondria. With these probes, we first observed dynamic physical lysosome-mitochondrion interactions for over 13 min in live-cell dual-color super-resolution imaging.

Cited by 19 publications

References 0 publications

Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore

Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore

Combined Structural and Functional Imaging of the Kidney Reveals Major Axial Differences in Proximal Tubule Endocytosis

Targeted fluorescent imaging of a novel FITC-labeled PSMA ligand in prostate cancer

Contact Info

Product

Resources

About