Cell phenotypic changes of mouse connective tissue fibroblasts (L-929) to poly(ethylene glycol)-based gels

Abstract

a Cellular responses to various gels fabricated by photoinitiated crosslinking using acrylated linear and multi-arm poly(ethylene glycol) (PEG)-based and poly(propylene glycol)-b-poly(ethylene glycol) precursors were investigated. While no protein adsorption and cell adhesion were observed on the hydrophilic PEG-based gels, protein adsorption and cell adhesion did occur on the more hydrophobic gel generated from the block copolymer precursor. Murine fibroblast viability on the poly(ethylene glycol)-based gels was studied in the course of 72 h and the results indicated no cytotoxicity. In a systematic study, extraand intracellular metabolites of the murine fibroblasts cultured on these PEG-based gels were examined by GC-MS. Distinct intra-and extracellular changes in primary metabolism, namely amino acid metabolism, glycolysis and fatty acid metabolism, were observed. Cells cultured on the polymeric gels induced more intense intracellular changes in the metabolite profile by means of higher metabolite intensities with time in comparison to cells cultured on the reference substrate (tissue culture polystyrene). In contrast, extracellular changes of metabolite intensities were comparable.

Enzymatically Cross-Linked Hyperbranched Polyglycerol Hydrogels as Scaffolds for Living Cells

“…Figure a shows that L929 cells remained viable (98%) on the hPG-HPA@5% gel surfaces for a cultivation period of 2 days. Although the round-shaped cell morphology (Figure b) indicates that L929 cells could not adhere to the surface due to the nonfouling characteristics of the gels, − cellular viability was not affected during the time of observation, which is in good accord with the published data of other polyglycerol gels. , The mechanism of protein/cell nonfouling effect has not been fully understood until now, but some empiric data indicate that the surface hydrophilicity plays an important role. − Interestingly, the Cooper-White group recently invented a simple method to enhance cell adhesion on the cell nonfouling PEG gels by incorporating a peptide fragment of fibronectin (Fn), a protein known to enhance cell adhesion . Inspired by this finding, a cell adhesion study was also performed on hPG-HPA@5% gels with the addition of 360 μg/mL Fn; Figure c showed that cell attachment was then observed on the surface, which is in line with the finding by Cooper-White et al On the other hand, when the degree of HPA substitution of hPG was increased from 5% to 10%, the cells on the gel surface were still highly viable (99%) for a 48 h cultivation period (Figure d).…”

“…Cells were not only cultured on the gel surface but also encapsulated into hPG-HPA@5% gels in order to assess the possibility of hosting living cells for perspective therapeutic use. After encapsulation for 48 h, 51% cell viability was observed (Figure a), which is expected because of the gel’s nonadhesive property and the confined polymeric networks to suppress cell growth. ,− Surprisingly, the supplemented Fn greatly enhanced the cell viability up to 99%. Cells inside gels, however, did not spread even with the addition of Fn (Figure b and c) because the loaded cells were strictly restricted within gels.…”

Functional PEG-Hydrogels Convey Gold Nanoparticles from Silicon and Aid Cell Adhesion onto the Nanocomposites

“…First of all, it should be noted that we are very aware that cell adhesion, spreading, and migration heavily depend on the chemistry, topography, and elasticity of the biointerface, as we have studied these relationships in great detail during the last several years. − …”

Surface Patterning of Gold Nanoparticles on PEG-Based Hydrogels to Control Cell Adhesion

“…While 3BC-UV, PEG575-UV and 8PEG-UV hydrogels are formed through the same crosslinking method, the chemical structure of 3BC is different from that of the pure PEG-hydrogels PEG575 and 8PEG. The PPG-block in 3BC is much more hydrophobic than PEG-segments, which makes this gel more hydrophobic in comparison to PEG575-UV and 8PEG-UV hydrogels [ 33 ].…”