Cell population kinetics and ploidy rate of early focal lesions during hepatocarcinogenesis in the rat

Castelain, Ph.; Deleener, A.; Kirsch‐Volders, Micheline; Barbason, H.

doi:10.1038/bjc.1989.374

Cited by 14 publications

(2 citation statements)

References 21 publications

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“…From the present study, there was a significant decrease in DNA density in group B which is indicative of cellular degeneration. This is in corroboration with Castelain and coworkers who worked on cell population kinetic and ploidy rates during hepatocarcinogenesis (Castelain et al, 1989). In the coconut milk and omeprazole groups, the DNA density significantly increased in a dose dependent manner as shown in the photomicrographs.…”

Section: Discussionsupporting

confidence: 89%

The histochemical and biochemical effects of coconutmilk in Wistar rats with ethanol-induced gastric ulcer

Tolulope¹,

David²,

Sylvester³

et al. 2019

Anat. J. Afr.

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This study aimed at investigating the histochemical and biochemical effects of coconut milk in Wistar rats’ model of ethanol-induced gastric ulcer with a view to evaluating the antiulcerogenic potential of coconut milk in the management of gastric ulcer. Thirty male Wistar rats weighing between 150-180g were recruited for the study and divided into six groups of 5 rats each. All the groups were fasted for 24-hours after which group-1 received single oral administration of normal saline at 5ml/kg, while groups 2-6 received equivalent amount of ethanol. Twenty-four hours later, Groups-3, 4, 5 received oral graded doses of coconut milk at 30, 50, 70 mg/kg respectively while group 6 received omeprazole administration at 10 mg/kg every 12-hours for a period of 21 days. The ulcer index as well as Prostalglandin-E2 and Nitric Oxide concentrations was significantly (p < 0.05) lowered following treatment with omeprazole and coconut milk, when compared with the ethanol group. Coconut milk administration also attenuated the ethanol-induced histochemical distortions of the gastric pylorus. This study concluded that coconut milk administration ameliorated ethanol-induced gastric ulcer and serves as a potential choice in the treatment of the condition.Keywords: Gastric ulcer, Ethanol, Coconut Milk, Omeprazole, Histochemical, Biochemical

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Section: Discussionsupporting

confidence: 89%

The histochemical and biochemical effects of coconutmilk in Wistar rats with ethanol-induced gastric ulcer

Tolulope¹,

David²,

Sylvester³

et al. 2019

Anat. J. Afr.

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“…Instead of considering a population of organisms, we can refer to a population of cells within multicellular organisms. This interpretation of carcinogenesis (model 4 in our scheme above) is not new, having been proposed by several authors since the 1970s (35)(36)(37)(38)(39). In addition, as reading these papers makes clear, cell selection was an integral part of mutational theory because mutation alone was incapable of producing experimental cancers if not followed by cell selection.…”

Section: Mathematical Representationsmentioning

confidence: 84%

Models of carcinogenesis: an overview

Víneis¹,

2010

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At least five coherent models of carcinogenesis have been proposed in the history of cancer research in the last century. Model 1 is mainly centered around mutations, and its main focus is on the chemical environment, radiation and viruses. Model 2 has to do mainly with genome instability and it focuses on familiality. Model 3 is based on non-genotoxic mechanisms, and clonal expansion and epigenetics are its main features. We propose a fourth model, which can encompass the previous three, based on the concept of a 'Darwinian' cell selection (we clarify that the term Darwinian needs to be used cautiously, being a short cut for 'somatic cellular selection'). Finally, a fifth model has recently become popular, based on the concept of 'tissue organization'. We describe examples of the five models and how they have been formalized mathematically. The five models largely overlap, both scientifically and historically, but for the sake of clarity, it is useful to treat them separately. We also argue that the five models can be included into a simpler scheme, i.e. two types of models: (i) biological changes in the epithelium alone lead to malignancy and (ii) changes in stroma/extracellular matrix are necessary (along with changes in epithelium) for malignancy. Our description, though simplified, looks realistic, it is able to capture the historical sequence of carcinogenesis theories in the last century and can serve as a frame to make research hypotheses more explicit.

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Nuclear ploidy of normal and neoplastic hepatocytes from woodchuck hepatitis virus-infected and uninfected woodchucks

1994

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Flow cytometric analysis of the ploidy of normal and neoplastic hepatocyte nuclei obtained from adult woodchucks, a model of human hepadnavirus-induced hepatocellular carcinoma, was performed. All 36 samples of nuclei from non-neoplastic liver from woodchuck hepatitis virus-infected or uninfected liver were diploid, indicating that age-related nuclear polyploidization does not occur in this species, unlike other rodents. Individual or multiple hepatic neoplasms were obtained from each of 14 woodchuck hepatitis virus-infected woodchucks. Nineteen samples of hepatocellular carcinoma and eight adenomas were examined. Aneuploid nuclei were detected in 10 of the hepatocellular carcinomas and three of the adenoma samples. Similar DNA indexes, ranging from 1.11 to 1.22, were found in 7 of the 10 aneuploid HCCs and all 3 aneuploid adenomas. Nine of the 19 hepatocellular carcinoma samples and 5 of the 8 adenomas were diploid. Four of the diploid hepatocellular carcinomas had increased proportions of tetraploid nuclei. The presence of aneuploid nuclei was not related to histological appearance of the neoplasms or serum gamma-glutamyltranspeptidase levels. Because none of the hepatocellular carcinomas metastasized, the presence of aneuploidy could not be related to biological behavior. We determined the proportion of uninucleate and binucleate hepatocytes in hepatocellular carcinoma and nonneoplastic liver. Approximately 7% of hepatocytes were binucleate in nonneoplastic liver from woodchuck hepatitis virus-infected and uninfected liver. Only 2% of malignant hepatocytes were binucleate. The results of this study indicate that aneuploidy is a common change in hepatic neoplasms from woodchuck hepatitis virus-infected woodchucks.

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Cell population kinetics and ploidy rate of early focal lesions during hepatocarcinogenesis in the rat

Cited by 14 publications

References 21 publications

The histochemical and biochemical effects of coconutmilk in Wistar rats with ethanol-induced gastric ulcer

The histochemical and biochemical effects of coconutmilk in Wistar rats with ethanol-induced gastric ulcer

Models of carcinogenesis: an overview

Nuclear ploidy of normal and neoplastic hepatocytes from woodchuck hepatitis virus-infected and uninfected woodchucks

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