Cell Population Kinetics of the Recognizable Erythroid Cells in the Rat

Abstract

The cell population kinetic parameters defining a simple model of the recognizable part of the erythroid system have been determined. Experimental results using tritiated thymidine and radioactive iron autoradiography have provided estimates of the number of cell divisions, transit times and flow rates for all the recognizable stages of the erythroid system. The accuracy of the estimates and the validity of the model employed are discussed.

The Bone Marrow Micronucleus Assay: Rationale for a Revised Protocol

“…The process of erythropoietic differentiation and its relationship to the micronucleus assay is shown in Figure 2a (77,79,81,83) during which they undergo various structural and metabolic changes, including the breakdown and metabolism of ribosomal RNA, As a consequence of these events, the staining properties of the cell change until the cell is no longer recognizable as a newly formed erythrocyte and, hence, is classified as an RBC. The RBC survives for weeks or months, depending upon the species, so that the effect of any short-term clastogenic insult is greatly diluted by the large pool of preexisting unaffected RBCs.…”

“…This observation agrees well with several other studies of the kinetics of micronucleus production. Differences between the theoretical and observed minimum times for the duration of the G2-mitosis period of the last blast cell cycle(47) plus the OE transit time (77,79) i.e., 5-7 hr versus 8-12 hr, may be due to (1) mitotic delay induced by the agent, (2) the need to accumulate sufficient cells to reach a detectable limit, or (3) the time required for the uptake and metabolism of the agent. Indeed, the time necessary for the uptake of the agent and, in some cases, its conversion to its active metabolite may extend the period before the appearance of micronuclei in the PCE stage considerably.…”

The granulocytopoiesis in the spleen of newborn rats

“…The following analysis is based on the ,,random model" of haematopoiesis, as it was originally described by Tarbutt and Blackett (1968), assuming an exponential phase distribution of cells within the cell cycle.…”

A Cell Kinetic Study on the Cancer Problem Based on the Automatic Control Theory Using Digital Simulation

“…(1968), Tarbutt and Blackett (1968), Wheldon, Kirk and Gray (1973), and Wheldon, Kirk and Orr (1974) deal with the clear method of representation by block diagrams and also show mathematical statements for the cell renewal process by using differential equations. Hahn (1966), moreover, introduces the state space description when analyzing the cell proliferation and von Bronk, Dienes and Paskin (1968), Harris (1959) and Josifescu and Tautu (1973) bring in the stochastic theory of cell kinetics.…”