Cell proliferation at different sites along the length of the rat colon

Sunter, J. P.; Watson, Alexander; Wright, Nicholas; Appleton, David R.

doi:10.1007/bf02889015

Cited by 48 publications

(23 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In each zone sampled, 3000 neoplastic epithelial cells were counted, and the percentages of mitotic (late prophases, metaphases and anaphases) and of [3H]-TdRlabelled cells (5 or more grains over the nucleus) were calculated. The mean values for the different zones in the different types of tumours were compared with each other, and writh those for the normal mucosa (Sunter et al, 1979) by means of t tests.…”

Section: Methodsmentioning

confidence: 99%

“…The distances were measured with an eyepiece graticule. A number of lesions found at several different times after the start of DMH injections, and ranging in size between 3 and 6 mm in greatest diameter, were analysed and the results are presented in Table I (Sunter et al, 1979).…”

Section: Colonic Tumours and Their Distributionmentioning

confidence: 99%

“…Techniques used have included calculation of the mitotic index (Im) and the 3H-thymidine ([3H]-TdR)-labelling index (Is), together with estimation of the major cell-cycle parameters from fraction-of-labelled-mitoses (FLM) experiments. The different tumour types have been compared in terms of these parameters, both with one another and with the normal colonic mucosa in the region where each type was most prevalent (Sunter et al, 1979).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Cell proliferation of colonic neoplasms in dimethylhydrazine-treated rats

Sunter¹,

Hull²,

Appleton³

et al. 1980

Br J Cancer

View full text Add to dashboard Cite

Summary.-We have measured mitotic indices and 3H-thymidine-labelling indices for the colonic epithelial tumours induced in rats by the administration of dimethylhydrazine (DMH). The fraction-of-labelled-mitoses (FLM) technique has been used to estimate the duration of the cell-cycle phases.In general, mitotic and labelling indices in the tumours are similar to those in the proliferation zone of the normal crypt epithelium; lesions considered to be least well differentiated on histological grounds appear to have the lowest mean labelling index. Benign tumours and the different types of malignant tumours have mean cell-cycle times about half those of the normal mucosa.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Colonic Tumours and Their Distributionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Cell proliferation of colonic neoplasms in dimethylhydrazine-treated rats

Sunter¹,

Hull²,

Appleton³

et al. 1980

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…In these investigations, a mid-crypt proliferative zone was reported in both animals (Ahnen et al, 1987;Altmann, 1987;Chang and Hoff, 1980;McGarrity et al, 1988;Sato and Ahnen, 1992;Yamada et al, 1992). The proliferative zone in the mouse and rat cecum is reported to be the same as that found in distal colon, with division occurring at the crypt base (Chang and Hoff, 1980;Sunter et al, 1979;Yamada et al, 1992). Conflicting results from studies of proliferative patterns in the human colon have been reported.…”

mentioning

confidence: 97%

“…It was here that the first theories of basally-located cells that divide and mature as they migrate to the surface epithelium were formed (Cheng and Leblond, 1974;Meissier and Leblond, 1960;Merzel and Leblond, 1969). These theories were then expanded to encompass distal colon after autoradiography experiments were performed in mouse descending colon (Chang and Leblond, 1971;Chang and Nadler, 1975;Deschner and Maskens, 1982) and rat descending colon (Ahnen et al, 1987;McGarrity et al, 1988;Sunter et al, 1978Sunter et al, , 1979.…”

mentioning

confidence: 99%

Epithelial cell dynamics in rabbit cecum and proximal colon P1

Grant

Specian

2001

Anat. Rec.

View full text Add to dashboard Cite

The large intestine of mammals has long been viewed as an osmoregulatory organ, and evidence suggests that fluid and solute transport mechanisms within the intestine are heterogenous, varying depending on the particular segment involved. Variations in function are often matched by morphological correlates, but despite the widespread use of rabbit large intestine as an experimental model, there is a lack of knowledge about the cellular makeup and dynamics in the colonic mucosal epithelium. The presence of mitotic figures and immunohistochemical localization of proliferating cell nuclear antigen (PCNA) were used to identify the proliferative zone(s). Cellular migration patterns were determined through the use of the thymidine analog 5-bromo-2-deoxyuridine (BrdU) over a 24-, 48-, and 72-hr period. Apoptotic nuclei were identified utilizing terminal deoxynucleotidyl transferase d-UTP nick-end labeling (TUNEL). Both cecum and the initial portion of the proximal colon (P1) exhibited a proliferative zone at or near the crypt base, and migration proceeded upwards toward the surface epithelium lining the intestinal lumen, where apoptosis occurred Turnover time of crypt columnar cells was determined to be about 3 days; that of mucous cells was estimated to be about 5 weeks. Rabbit cecum and proximal colon P1 are similar in their cellular morphology and epithelial cell kinetics. In both, the major proliferative zone is located at or near the crypt base, from which crypt columnar cells migrate toward the lumenal surface epithelium over a period of 3 days. Goblet cell turnover rate is much slower than that of columnar cells.

show abstract

Proliferation of goblet cells and vacuolated cells in the rabbit distal colon

Grant

Specian

1998

Anat. Rec.

View full text Add to dashboard Cite

Previous studies of colonic epithelial cell kinetics in mice and rats revealed a pattern similar to small intestine, where basally located stem cells proliferate, differentiating as they migrate towards the surface epithelium. Vacuolated and goblet cells are assumed to co-migrate at the same rate. The present study indicates that rabbit distal colon has more complicated epithelial cell kinetics. The zone of proliferation was detected immunohistochemically using proliferating cell nuclear antigen (PCNA) and confirmed with the use of colchicine to arrest dividing cells in metaphase. Migrating cells were tracked from the zero-hour position (PCNA labeling, mitosis) to positions 24, 48, 72 hrs by monitoring cell migration with the thymidine analog 5-Bromo-2-Deoxyuridine (BrdU). PCNA revealed a major proliferative zone in the upper third of the crypt column and the presence of mitotic figures after colchicine corroborated these results. Differentiated vacuolated cell proliferation was detected at three crypt sites: base, middle, and top of the crypt, while columnar cells arose from a population of dividing cells at the top of the crypt. Turnover of columnar and vacuolated cells occurred within 72 hrs. Goblet cells exhibited maximal proliferation at the crypt base and migrated at a much slower rate than the other cell types. In rabbit distal colon, populations of proliferating cells exist at multiple levels of the crypt column. Vacuolated and goblet cells differ in their labeling indices and migration rates, suggesting that the two cell types arise and migrate independently.

show abstract

Cell proliferation at different sites along the length of the rat colon

Cited by 48 publications

References 12 publications

Cell proliferation of colonic neoplasms in dimethylhydrazine-treated rats

Cell proliferation of colonic neoplasms in dimethylhydrazine-treated rats

Epithelial cell dynamics in rabbit cecum and proximal colon P1

Proliferation of goblet cells and vacuolated cells in the rabbit distal colon

Contact Info

Product

Resources

About