Cell proliferation induced by modified cationic dextran

Abstract

The interaction between oppositely charged membranes and polycations causes cell aggregation, loss of membrane fluidity, and membrane degeneration and may cause an increase of its permeability. Unfortunately, the interaction is the reason why the use of polycations in medicine is severely limited. Therefore, in this paper, we share our observations related to the action of 40-kDa dextran modified using glycidyltrimethylammonium chloride, resulting in increased fibroblast cell proliferation. Using viability and proliferation tests [3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, crystal violet, 3 H-thymidine incorporation], we have observed that cationic dextran derivatives exert a positive impact on nonepithelial cell proliferation in vitro. This phenomenon has been noted for human and mouse fibroblasts and several other nonepithelial cell lines. However, the effect seems to be most pronounced for fibroblast cell lines. The presented studies allow to examine the impact of the polymer structure and the methods of its cationic modification on this newly observed phenomenon. The observation is unique because positively charged macromolecules usually exhibit high toxicity in all cell types in vitro.

Growth of Lactic Acid Bacteria on Gold—Influence of Surface Roughness and Chemical Composition

“…Three cationic dextran derivatives differing in a molar mass confirmed and characterized by gel permeation chromatography (GPC) method (detailed data are shown in the Supplementary Material, Figure S2 ) were selected for this study. In our earlier publications [ 31 , 32 ], we have discussed the effect of positive charge and molecular weight of polysaccharides on their biological activity and toxicity. Due to the electrostatic mechanism of nanocoating formation on the surface of gold, we imposed the synthesis conditions that the polymer charge should be fixed while the molar mass will be a variable.…”

“…Due to the electrostatic mechanism of nanocoating formation on the surface of gold, we imposed the synthesis conditions that the polymer charge should be fixed while the molar mass will be a variable. Such an approach is also justified by the fact that the charge will affect the toxicity, which may distort the obtained results [ 32 ]. Therefore, the zeta potential of the respective solutions was measured to confirm that we have obtained polymers with the desired charge.…”

“…A similar strategy was used for new material, but currently, the effect of the molecular weight (6, 40, and 100 kDa) of cationic dextrans on surface interactions with the lactic acid bacteria strains will also be studied. It is known that such polycations have the ability to model biological activity and human cell growth [ 31 , 32 ], which may be related to the interaction with a negatively charged biological membrane or negatively charged proteins and polypeptides.…”

Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives

“…For beta-glucans from both sources, the cationizations were performed in the manner used to obtain the cationic dextran described in our earlier work [ 20 , 42 ]. Briefly, 200 mg of beta-glucan was dissolved in 50 mL of distilled water containing an additional 200 mg of NaOH.…”

Pioglitazone-Loaded Nanostructured Hybrid Material for Skin Ulcer Treatment

“…Literature reports show inhibition [34] or acceleration [11] of cell proliferation by this compound depending on the circumstances and incubation time. Such time depending variability of proliferation-related properties may involve interaction of compound with growth factors as it is in the case of polycations [35]. To study the influence of NP-PIO_B/Ch(15) system on the fibroblasts motility and woundhealing properties the ex vivo "scratch" assay was performed (see Figure 11).…”