Cell Proliferation Inhibition in Remitting Multiple Sclerosis CSF

Abstract

Sixty percent of the CSF samples from 21 multiple sclerosis (MS) patients in remission inhibited cellular proliferation of a variety of neuronal and glial cell lines. No inhibition was observed with CSF from relapsing or progressive MS patients (0/19). Two of 82 samples from stationary MS patients were positive. No inhibition was found in MS sera. The CSF samples had no effect on myelination of mouse organotypic cerebellar cultures. Like MS cellular proliferation and subsequent lesion formation, the ‘deactivation’ of the lesion also is an active defined process, rather than just the cessation of the immunological events. Furthermore, approximately 20% of samples from Huntington’s, Alzheimer’s and Parkinson’s disease patients were also positive. However, no inhibition was seen in CSF from amyotrophic lateral sclerosis patients or controls.

Healthy human CSF promotes glial differentiation of hESC-derived neural cells while retaining spontaneous activity in existing neuronal networks

“…Meanwhile, several reports describe the effects of human CSF on rodent or chicken-derived neural cultures. These studies report that CSF derived from: 1) ALS patients increase the viability of cells (Nagaraja et al, 1994), 2) epilepsy patients enhance the neurite outgrowth (Akoev et al, 1996), 3) MS patients induce cell death and inhibit proliferation (Cid et al, 2003; Westall and Seil, 2006), and 4) from traumatic injury patients inhibit neuronal network function (Otto et al, 2009) of animal derived neural cultures. However, currently there are no reports describing the effects of CSF derived from neurologically healthy individuals on the neural cultures.…”