Cell Proliferation of Diploid and Tetraploid H1 (ES) Cells in L15F10 Medium without Leukemia Inhibitory Factor

Fujikawa-Yamamoto, Kohzaburo; Miyagoshi, Minoru; Yamagishi, H; Luo, Xue‐Gang

doi:10.1508/cytologia.73.259

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“…It was reported that the DNA content of 4H1 (−) cells decayed gradually 15 . Decay and abrupt increase of DNA content of 4H1 (−) cells during long‐term culturing were observed in this study.…”

Section: Discussionsupporting

confidence: 61%

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Different responses between diploid and tetraploid H1 embryonic stem cells appeared during long-term culturing in L15F10 medium without leukemia inhibitory factor

et al. 2010

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To examine the alteration in cellular characteristics of polyploid embryonic stem (ES) cells during long-term culturing without leukemia inhibitory factor (LIF), mouse diploid and tetraploid H-1 (ES) cells (2H1 and 4H1 cells, respectively) were cultured without LIF for approximately 5 months. 2H1 and 4H1 cells were adapted to the medium without LIF by decreasing the concentration for several passages, and they were denoted as 2H1(⁻) and 4H1(⁻) cells, respectively. DNA content of 4H1(⁻) cells decreased gradually in the early stage, increased abruptly in the second stage, and then was maintained for a long time. 4H1(⁻) cells exhibited longer doubling time and equivalent phase fraction compared with those of 2H1(⁻) cells. The G₁ phase fractions of 2H1(⁻) and 4H1(⁻) cells were increased compared with that of 2H1 cells. Cellular morphology and pluripotency were maintained in 4H1(⁻) cells but not in 2H1(⁻) cells. 2H1(⁻) cells showed a cell population consisting of several kinds of cells, and they lost alkaline phosphatase activity, suggesting that the cells had differentiated. 4H1(⁻) cells, however, exhibited alkaline phosphatase activity and formed teratocarcinoma in mouse abdomen, suggesting that the cells maintained their pluripotency in the medium without LIF.

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Section: Discussionsupporting

confidence: 61%

“…We reported that the pluripotency was maintained for several weeks in both diploid and tetraploid H1 cells in the medium without LIF 15 . The pluripotency disappeared in long‐term culturing for 2H1 (−) cells but not for 4H1 (−) cells in this study.…”

Section: Discussionmentioning

confidence: 47%