Cell proliferation of Paramecium tetraurelia on a slow rotating clinostat

Sawai, Satoe; Mogami, Yoshihiro; Baba, Shoji A.

doi:10.1016/j.asr.2007.02.023

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…This indicates that our procedure for measuring oxygen consumption using the sensor does not require any correction for heterogeneity of P O2 in the chamber. Although the cells are focused on in order to record swimming some distance away from the sensor surface at the top of the columnar space, it seems safe to regard them as being representative in terms of the homogenous distribution of Paramecium cells usually found in a chamber of such small dimensions as used in Sawai et al (Sawai et al, 2007). Fig.…”

Section: Resultsmentioning

confidence: 99%

Substantial energy expenditure for locomotion in ciliates verified by means of simultaneous measurement of oxygen consumption rate and swimming speed

Katsu-Kimura

Nakaya

Baba

et al. 2009

Journal of Experimental Biology

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SUMMARY In order to characterize the energy expenditure of Paramecium, we simultaneously measured the oxygen consumption rate, using an optic fluorescence oxygen sensor, and the swimming speed, which was evaluated by the optical slice method. The standard metabolic rate (SMR, the rate of energy consumption exclusively for physiological activities other than locomotion)was estimated to be 1.18×10–6 J h–1cell–1 by extrapolating the oxygen consumption rate into one at zero swimming speed. It was about 30% of the total energy consumed by the cell swimming at a mean speed of 1 mm s–1, indicating that a large amount of the metabolic energy (about 70% of the total) is consumed for propulsive activity only. The mechanical power liberated to the environment by swimming Paramecium was calculated on the basis of Stokes' law. This power, termed Stokes power, was 2.2×10–9 J h–1 cell–1, indicating extremely low efficiency (0.078%) in the conversion of metabolic power to propulsion. Analysis of the cost of transport (COT, the energy expenditure for translocation per units of mass and distance) revealed that the efficiency of energy expenditure in swimming increases with speed rather than having an optimum value within a wide range of forced swimming, as is generally found in fish swimming. These characteristics of energy expenditure would be unique to microorganisms, including Paramecium, living in a viscous environment where large dissipation of the kinetic energy is inevitable due to the interaction with the surrounding water.

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Section: Resultsmentioning

confidence: 99%

Substantial energy expenditure for locomotion in ciliates verified by means of simultaneous measurement of oxygen consumption rate and swimming speed

Katsu-Kimura

Nakaya

Baba

et al. 2009

Journal of Experimental Biology

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“…[40][41][42] about the pattern formation in suspensions of Tetrahymena and Chlamydomonas subject to different gravity conditions. Further results are due to Sawai et al [43] who investigate the proliferation of Paramecium under simulated microgravity, to Mogami et al [44] who report an investigation of the formed patterns by Tetrahymena and Chlamydomonas as well as a physiological comparison, to Takeda et al [45] who give an explanation of the gravitactic behavior of single cells of Paramecium in terms of the swimming velocity and swimming direction, to Mogami et al [46] who present theory and experiments of two mechanisms of gravitactic behavior for microorganisms, and to Itoh et al [47] who investigate the modification of bioconvective patterns under strong gravitational fields.…”

Section: Introductionmentioning

confidence: 87%

Bioconvective Linear Stability of Gravitactic Microorganisms

Pérez-Reyes¹,

Dávalos‐Orozco²

2019

Heat and Mass Transfer - Advances in Science and Technology Applications

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Interesting results on the linear bioconvective instability of a suspension of gravitactic microorganisms have been calculated. The hydrodynamic stability is characterized by dimensionless parameters such as the bioconvection Rayleigh number R, the gyrotaxis number G, the motility of microorganisms d, and the wavenumber k of the perturbation. Analytical and numerical solutions are calculated. The analytical one is an asymptotic solution for small wavenumbers (and for any motility number) which agrees very well with the numerical solutions. Two numerical methods are used for the sake of comparison. They are a shooting method and a Galerkin method. Marginal curves of R against k for fixed values of d and G are presented along with curves corresponding to the variation of the critical values of R c and k c. Moreover, those critical values are compared with the experimental data reported in the literature, where the gyrotactic algae Chlamydomonas nivalis is the suspended microorganism. It is shown that the agreement between the present theoretical results and the experiments is very good.

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“…Authors observed that the growth rate of Paramecium tetraurelia increased in microgravity and simulated-microgravity 1,18,19 as well as Paramecium biaurelia cells swam faster under altered gravity induced by clinorotation. 20 However Sawai et al 21 point out that P. tetraurelia reduced proliferation rhythm under slow-clinorotation and in this condition the swimming velocity decreased too.…”

Section: Introductionmentioning

confidence: 99%

Effects of altered gravity induced by clinorotation on the cholinesterase activity of the non-sentient model Paramecium primaurelia (Protozoa)

et al. 2018

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Compounds known as chemical mediators, including acetylcholine, have been found not only in animals and humans, but also in living organisms, like protozoa, which lack nervous system. In Paramecium primaurelia has been described a cholinergic system, which is proven to play an important role in cell-cell interactions during its developmental cycle. In our work we investigated the effects of exposure to simulated microgravity (3D Random Positioning Machine, 56 rpm, 10 -6 g) on the cholinesterase activity of the eukaryote unicellular-organism alternative-model P. primaurelia. Our results show that the exposure of P. primaurelia to microgravity for 6 h, 24 h, 48 h affects the localization and the amount of cholinesterase activity compared to cells grown under Earth gravity conditions (1 g). However, these effects are transient since P. primaurelia restores its normal cholinesterase activity after 72 h under microgravity conditions, as well as cells exposed up to 72 h to microgravity and then placed under terrestrial gravity for 48 h.

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Cell proliferation of Paramecium tetraurelia on a slow rotating clinostat

Cited by 4 publications

References 17 publications

Substantial energy expenditure for locomotion in ciliates verified by means of simultaneous measurement of oxygen consumption rate and swimming speed

Substantial energy expenditure for locomotion in ciliates verified by means of simultaneous measurement of oxygen consumption rate and swimming speed

Bioconvective Linear Stability of Gravitactic Microorganisms

Effects of altered gravity induced by clinorotation on the cholinesterase activity of the non-sentient model Paramecium primaurelia (Protozoa)

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