Cell protrusions and contractions generate long-range membrane tension propagation

Belly, Henry De; Yan, Shannon; Rocha, Hudson Borja da; Ichbiah, Sacha; Town, Jason P.; Zager, Patrick J.; Estrada, D. Fernando; Meyer, Kirstin; Turlier, Hervé; Bustamante, Carlos; Weiner, Orion D.

doi:10.1016/j.cell.2023.05.014

Cited by 60 publications

(34 citation statements)

References 65 publications

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“…In a hypertonic environment, free water flows out of the cells and the cells shrink, which corresponds to stage II in Figure . When cells contract to the volume set point, the transportation system activated by cell contraction is inactive or slightly active . In other words, the rate of loss of free water in the cells slows down.…”

Section: Resultsmentioning

confidence: 99%

Deep-Learning Terahertz Single-Cell Metabolic Viability Study

Yang,

Shi,

Wei

et al. 2023

ACS Nano

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Cell viability assessment is critical, yet existing assessments are not accurate enough. We report a cell viability evaluation method based on the metabolic ability of a single cell. Without culture medium, we measured the absorption of cells to terahertz laser beams, which could target a single cell. The cell viability was assessed with a convolution neural classification network based on cell morphology. We established a cell viability assessment model based on the THz-AS (terahertz-absorption spectrum) results as y = a = (x – b) c , where x is the terahertz absorbance and y is the cell viability, and a, b, and c are the fitting parameters of the model. Under water stress the changes in terahertz absorbance of cells corresponded one-to-one with the apoptosis process, and we propose a cell 0 viability definition as terahertz absorbance remains unchanged based on the cell metabolic mechanism. Compared with typical methods, our method is accurate, label-free, contact-free, and almost interference-free and could help visualize the cell apoptosis process for broad applications including drug screening.

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Section: Resultsmentioning

confidence: 99%

Deep-Learning Terahertz Single-Cell Metabolic Viability Study

Yang,

Shi,

Wei

et al. 2023

ACS Nano

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“…It is also important to remind ourselves that while large-length-scale mechanical heterogeneities of membrane tension are not expected for a fluid membrane, recent studies have shown and explained that heterogeneity is possible and controllable due to slow or fast flow of tension. When forces engage the cytoskeleton, they can travel faster than when applied solely to the membrane . Rapid vesicle turnover in neuronal terminals has been shown to lead to rapid equilibration of membrane tension (within seconds).…”

Section: Receptor Clustering Tension Equilibration and Mechanoregulationmentioning

confidence: 99%

The Plasma Membrane and Mechanoregulation in Cells

Mukhopadhyay,

Mandal,

Chakraborty

et al. 2024

ACS Omega

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Cells inhabit a mechanical microenvironment that they continuously sense and adapt to. The plasma membrane (PM), serving as the boundary of the cell, plays a pivotal role in this process of adaptation. In this Review, we begin by examining well-studied processes where mechanoregulation proves significant. Specifically, we highlight examples from the immune system and stem cells, besides discussing processes involving fibroblasts and other cell types. Subsequently, we discuss the common molecular players that facilitate the sensing of the mechanical signal and transform it into a chemical response covering integrins YAP/TAZ and Piezo. We then review how this understanding of molecular elements is leveraged in drug discovery and tissue engineering alongside a discussion of the methodologies used to measure mechanical properties. Focusing on the processes of endocytosis, we discuss how cells may respond to altered membrane mechanics using endo- and exocytosis. Through the process of depleting/adding the membrane area, these could also impact membrane mechanics. We compare pathways from studies illustrating the involvement of endocytosis in mechanoregulation, including clathrin-mediated endocytosis (CME) and the CLIC/GEEC (CG) pathway as central examples. Lastly, we review studies on cell–cell fusion during myogenesis, the mechanical integrity of muscle fibers, and the reported and anticipated roles of various molecular players and processes like endocytosis, thereby emphasizing the significance of mechanoregulation at the PM.

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“…Similarly, septin rings help compartmentalize plasma membranes at the bases of cilia and the cleavage furrow during cell division [28][29][30], while tight junctions maintain apical and basolateral membranes in epicedial cells [22]. Intracellular cytoskeleton or extracellular matrix attaches plasma membranes to form dynamic fence-like structures [31,32]. These fences permit lipids to flow in and out but confine the mobile proteins for a significant time before they escape the fences, creating highly heterogeneous protein distributions in plasma membranes [33].…”

Section: Lateral Lipid Flow Between Different Membrane Domains Throug...mentioning

confidence: 99%

“…Membrane tension gradient has long been recognized as a major driving force for lateral lipid flow within the same membrane [23,24,32,39] and is expected to also serve as a driving force for the lipid flow across different membranes through LTPs or leaflets through scramblases (Figures 1a & 1f). However, the membrane tension of some plasma membranes varies significantly in different areas tested without significant lipid flow between them [23,24,40,41].…”

Section: Lateral Lipid Flow Between Different Membrane Domains Throug...mentioning

confidence: 99%

“…In contrast, autophagosomes bear few integral proteins [44]. Consequently, the osmotic membrane tension can be as high as 0.4 pN/nm, compared to the reported cell membrane tension of 0–0.4 pN/nm [23,32,39,45]. Therefore, a physiological protein density gradient can generate a high osmotic membrane tension.…”

Section: Lipid Transfer Through Lipid Transfer Proteins and Scramblasesmentioning

confidence: 99%

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Lipid osmosis, membrane tension, and other mechanochemical driving forces of lipid flow

Zhang,

Lin

2024

Preprint

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Nonvesicular lipid transport among different membranes or membrane domains plays crucial roles in processes such as lipid homeostasis and organelle biogenesis. However, the forces that drive such lipid transport are not well understood. We propose that lipids tend to flow towards the membrane area with a higher membrane protein density in a process termedlipid osmosis. This process lowers the membrane tension in the area when the lipid flow reaches equilibrium. We examine the thermodynamic basis and experimental evidence of lipid osmosis and the correspondingosmotic membrane tension. We predict that lipid osmosis can drive lipid flows between different membrane regions through lipid transfer proteins, scramblases, or other similar barriers that selectively pass lipids but not membrane proteins. We also speculate on the biological functions of lipid osmosis. Finally, we explore other driving forces for lipid transfer and describe potential methods and systems to further test our theory.

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Cell protrusions and contractions generate long-range membrane tension propagation

Cited by 60 publications

References 65 publications

Deep-Learning Terahertz Single-Cell Metabolic Viability Study

Deep-Learning Terahertz Single-Cell Metabolic Viability Study

The Plasma Membrane and Mechanoregulation in Cells

Lipid osmosis, membrane tension, and other mechanochemical driving forces of lipid flow

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