Cell-recycle batch process of Scheffersomyces stipitis and Saccharomyces cerevisiae co-culture for second generation bioethanol production

Ashoor, Selim; Comitini, Francesca; Ciani, Maurizio

doi:10.1007/s10529-015-1919-9

Cited by 13 publications

(4 citation statements)

References 17 publications

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“…Usually, the substrates are lignocellulosics or its hydrolysate, and the products are ethanol, butanol and so on. At present, bioethanol research has obtained certain achievements in processing techniques, such as control of oxygen transfer, 17 batch cells recycling fermentation, 65 and development of new strain combination. 66 , 67 Among these approaches, one-pot method aided with cellulase-inducing substrate satisfied short time and high production at the same time.…”

Section: Synthetic Microbial Consortia Composed Of Two Speciesmentioning

confidence: 99%

Design, analysis and application of synthetic microbial consortia

Jia

Liu

Song

et al. 2016

Synthetic and Systems Biotechnology

100

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The rapid development of synthetic biology has conferred almost perfect modification on single cells, and provided methodological support for synthesizing microbial consortia, which have a much wider application potential than synthetic single cells. Co-cultivating multiple cell populations with rational strategies based on interacting relationships within natural microbial consortia provides theoretical as well as experimental support for the successful obtaining of synthetic microbial consortia, promoting it into extensive research on both industrial applications in plenty of areas and also better understanding of natural microbial consortia. According to their composition complexity, synthetic microbial consortia are summarized in three aspects in this review and are discussed in principles of design and construction, insights and methods for analysis, and applications in energy, healthcare, etc.

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Section: Synthetic Microbial Consortia Composed Of Two Speciesmentioning

confidence: 99%

Design, analysis and application of synthetic microbial consortia

Jia

Liu

Song

et al. 2016

Synthetic and Systems Biotechnology

100

View full text Add to dashboard Cite

show abstract

“…2a where different cell ratio of co-culture converted the sugar mixture into ethanol at different ethanol production rate. The outperformed productivity of co-culture system relative to single-strain culture in the mixed sugar fermentation is expected since dedicating one strain to consume all sugar mixture would lead to a longer fermentation time for the completed conversion of all sugars compared to having multiple strains as illustrated in several previous studies (Ashoor et al 2015;Karagöz and Özkan 2014;Yadav et al 2011;Suriyachai et al 2013). The co-culture kinetic model could be used for optimization of co-culture fermentation by predicting an optimal initial cell ratio of the co-culture in any given sugar mixture.…”

Section: Model-based Design Of Optimal Co-culture Systemmentioning

confidence: 94%

“…There are few studies that examined the effect of co-culture cell ratio and optimized cell ratio to maximize fermentation performance. However, the cell ratio optimization of co-culture was mostly relied on trial and errors and statistical analysis where large number of experiments is required (Ashoor et al 2015;Karagöz and Özkan 2014;Suriyachai et al 2013). This approach is cost and labor intensive as a new set of experiment has to be conducted every time sugar composition in the feedstock changes.…”

Section: Introductionmentioning

confidence: 99%

Model-based optimization of Scheffersomyces stipitis and Saccharomyces cerevisiae co-culture for efficient lignocellulosic ethanol production

Unrean

Khajeeram

2015

Bioresour. Bioprocess.

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Background:The utilization of both C 6 and C 5 sugars is required for economical lignocellulosic bio-based processes. A co-culture system containing multiple strains of the same or different organisms holds promise for conversion of the sugar mixture available in different lignocellulosic feedstock into ethanol.Results: Herein a co-culture kinetic model has been developed which can describe the co-cultivation of S. stipitis and S. cerevisiae for ethanol fermentation in mixed C 6 /C 5 sugars. The predicted fermentation kinetics and ethanol production performance agreed well with experimental results, thus validating the model. The co-culture kinetic model has been implemented to design the optimal cell ratio for efficient conversion of rice straw or sugarcane bagasse feedstock into ethanol. The results reveal that the optimal co-culture system could enhance ethanol titer by up to 26 %, and ethanol productivity by up to 29 % compared to a single-strain culture. The maximum ethanol titer and productivity reached by the optimized co-culture was 46 and 0.49 g/l h, respectively. Conclusion:The co-culture model described here is a useful tool for rapid optimization of S. stipitis/S. cerevisiae co-culture for efficient and sustainable lignocellulosic ethanol production to meet the economic requirements of the lignocellulosic ethanol industry. The developed modeling tool also provides a systematic strategy for designing the optimal cell ratio of co-culture, leading to efficient fermentation of the C 6 /C 5 sugars available in any biomass feedstock.

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“…Environmental protection agency (EPA) aimed a goal of 36 billion gallons of green fuel to be mixed with gasoline by 2022 perhaps ethanol blended gasoline considered to be substitute fuel with decreased emission of greenhouse gases (Clairotte et al 2013). Lignocellulosic biomass is a potential substrate for production of bioethanol fuel (Ashoor et al 2015). Rice (Oriza sativa) is one of the abundant, alternative, and third most staple grain crops in the world behind wheat and corn.…”

Section: Introductionmentioning

confidence: 99%

Improved physicochemical pretreatment and enzymatic hydrolysis of rice straw for bioethanol production by yeast fermentation

et al. 2017

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Lignocellulosic biomass such as agricultural and forest residues are considered as an alternative, inexpensive, renewable, and abundant source for fuel ethanol production. In the present study, three different pretreatment methods for rice straw were carried out to investigate the maximum lignin removal for subsequent bioethanol fermentation. The chemical pretreatments of rice straw were optimized under different pretreatment severity conditions in the range of 1.79-2.26. Steam explosion of rice straw at 170 °C for 10 min, sequentially treated with 2% (w/v) KOH (SEKOH) in autoclave at 121 °C for 30 min, resulted in 85 ± 2% delignification with minimum sugar loss. Combined pretreatment of steam explosion and KOH at severity factor (SF 3.10) showed improved cellulose fraction of biomass. Furthermore, enzymatic hydrolysis at 30 FPU/g enzyme loading resulted in 664.0 ± 5.39 mg/g sugar yield with 82.60 ± 1.7% saccharification efficiency. Consequently, the hydrolysate of SEKOH with 58.70 ± 1.52 g/L sugars when fermented with OBC14 showed 26.12 ± 1.24 g/L ethanol, 0.44 g/g ethanol yield with 87.03 ± 1.6% fermentation efficiency.

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Cell-recycle batch process of Scheffersomyces stipitis and Saccharomyces cerevisiae co-culture for second generation bioethanol production

Abstract: Batch cells recycling fermentation is an effective process to use Sch. stipitis/Sacc. cerevisiae co-culture for second generation ethanol production.

Cited by 13 publications

References 17 publications

Design, analysis and application of synthetic microbial consortia

Design, analysis and application of synthetic microbial consortia

Model-based optimization of Scheffersomyces stipitis and Saccharomyces cerevisiae co-culture for efficient lignocellulosic ethanol production

Improved physicochemical pretreatment and enzymatic hydrolysis of rice straw for bioethanol production by yeast fermentation

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