Cell repair after liver injury: An analysis of some metabolic conditions required for the stimulation of RNA synthesis in postischemic liver nuclei

Piccoletti, Roberta; Bernelli‐Zazzera, Aldo

doi:10.1002/jcp.1041150114

Journal Cellular Physiology

1983

DOI: 10.1002/jcp.1041150114

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Cell repair after liver injury: An analysis of some metabolic conditions required for the stimulation of RNA synthesis in postischemic liver nuclei

Roberta Piccoletti¹,

Aldo Bernelli‐Zazzera²

Abstract: Nuclei isolated from liver cells recovering from reversible (non-necrogenic) ischemia show an increased RNA synthesis. The postischemic effect is not abolished by previous adrenalectomy and occurs in fasted as well as in fed animals. Treatment with cycloheximide, at a dose that severely inhibits protein synthesis without primary effects on RNA synthesis, suppresses the postischemic stimulation of RNA synthesis even if cycloheximide is administered when stimulation is already well developed. Postischemic liver … Show more

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1989

1992

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Cited by 3 publications

References 28 publications

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Stress Proteins and Reperfusion Stress in the Livera

Bernelli‐Zazzera¹,

Cairo²,

Schiaffonati³

et al. 1992

Annals of the New York Academy of Sciences

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Blood reperfusion after temporary liver ischemia induces the expression of heat shock genes and the synthesis of heat shock proteins (hsps), in particular hsp 70. Induction requires a certain duration of ischemia, suggesting that cell damage before reperfusion is essential for activation of heat shock genes. The expression of the hsp 70 gene is preceded by activation of the cellular protooncogenes c-fos and c-jun. However, the product of these genes, which is transcription factor AP-1, seems unnecessary for activation of the hsp 70 gene, which does not require the integrity of protein synthesis. Hsp genes seem to behave as "early response genes," enabling the cell to respond to emergency situations.

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Stress Proteins and Reperfusion Stress in the Livera

Bernelli‐Zazzera¹,

Cairo²,

Schiaffonati³

et al. 1992

Annals of the New York Academy of Sciences

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Spermidine Level and Protein Synthesis are Coregulated in Nonproliferating Hepatocytes

Schulz

Gebhardt²,

Mecke³

1989

Biological Chemistry Hoppe-Seyler

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The relationship between polyamines and the rate of protein synthesis was investigated in nonproliferating cells: primary cultures of adult rat hepatocytes maintained in serum-free media, and treated with dexamethasone or dexamethasone + insulin. During the second day of culture, polyamine biosynthesis became induced along with the rate of protein synthesis. While the activity of ornithine decarboxylase and the intracellular concentration of putrescine increased only transiently and that of spermine declined, the rise of the protein synthetic rate was paralleled by that of the intracellular spermidine concentration. The polyamine analogue diaminopropanol specifically decreased spermidine content and the protein synthetic rate. The intracellular concentration of spermidine was found subject to tight homeostatic regulation, e.g. not being altered by the addition of up to ImM of this polyamine to the culture medium. In contrast, addition of putrescine or spermine led to an increase in their respective intracellular concentrations. These findings indicate that spermidine specifically of the polyamines is involved in protein synthesis in the intact hepatocyte. Moreover, spermidine may mediate part of the trophic action of dexamethasone and insulin upon cultured hepatocytes.

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Patterns of RNA and Protein Synthesis in Post-Ischemic Livers

Bernelli‐Zazzera

1989

Free Radical Research Communications

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The re-establishment of the blood supply to a formerly ischemic liver lobe, before the "point of no return" of the tissue is reached, induces a series of changes in protein and RNA metabolism that are functional to the repair of the damage suffered by the cells. Among these events there is the increase in synthesis of a group of proteins known as heat-shock (or stress) proteins, which are also induced in liver cells by different kinds of oxidative stress. The increase in synthesis of these proteins is largely due to the activation of their genes: some of these genes are also activated in cells stimulated to grow. These observations suggest a link between oxidative stress, repair of cell damage and cell multiplication.

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Cell repair after liver injury: An analysis of some metabolic conditions required for the stimulation of RNA synthesis in postischemic liver nuclei

Cited by 3 publications

References 28 publications

Stress Proteins and Reperfusion Stress in the Livera

Stress Proteins and Reperfusion Stress in the Livera

Spermidine Level and Protein Synthesis are Coregulated in Nonproliferating Hepatocytes

Patterns of RNA and Protein Synthesis in Post-Ischemic Livers

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