Cell–scaffold interactions in tissue engineering for oral and craniofacial reconstruction

Wang, Fushi; Cai, Xinjie; Shen, Ya; Meng, Liuyan

doi:10.1016/j.bioactmat.2022.10.029

Cited by 38 publications

(24 citation statements)

References 402 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Summarizing the obtained results, we can say that cell recruitment to the material structure from the surrounding tissues was observed in both the control and experimental specimens. This evidences the integration of the materials into the tissues [ 32 , 33 ]. Based on the literature data, it can be argued that the presence of MSCs in the studied materials contributed to this.…”

Section: Resultsmentioning

confidence: 96%

Biocompatibility Study of Hydrogel Biopolymer Scaffold with Encapsulated Mesenchymal Stem Cells

et al. 2023

View full text Add to dashboard Cite

One of the key and actively developing areas of regenerative medicine is tissue-engineering. There is no doubt that the use of tissue-engineering products can have a significant impact on the efficiency of repair of damaged tissues and organs. However, before being used in clinical practice, tissue-engineering products require thorough preclinical studies to confirm their safety and efficacy, both with in vitro models and in experimental animals. This paper presents preclinical studies of a tissue-engineered construct, based on a hydrogel biopolymer scaffold carrier (consisting of blood plasma cryoprecipitate and collagen) with encapsulated mesenchymal stem cells, to evaluate its biocompatibility in vivo. The results were analyzed using histomorphology and transmission electron microscopy. It was shown that when implanted into animal (rat) tissues, the implants were completely replaced by connective tissue components. We also confirmed that no acute inflammation occurred in response to the scaffold implantation. The observed processes of cell recruitment to the scaffold from the surrounding tissues, the active formation of collagen fibers and the absence of acute inflammation testified that the regeneration process was ongoing in the implantation area. Thus, the presented tissue-engineered construct shows promise for becoming an effective tool for regenerative medicine in the future and may be used, in particular, to repair soft tissues.

show abstract

Section: Resultsmentioning

confidence: 96%

Biocompatibility Study of Hydrogel Biopolymer Scaffold with Encapsulated Mesenchymal Stem Cells

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The results showed that the control alloy promoted an initial cellular extension, a width stretching among the surface, and reduced intracellular stress fibers accumulation. This effect could be explained due to the reduced surface area-to-volume ratio and decreased roughness of the flat alloy, as well as the enlarged cellular spreading, outlining complications for efficient cell migration [ 42 ]. However, the cellular morphology did not show the classical polygonal-like orientation appreciated for endothelial cells [ 43 ], despite the intercellular connections detected on the control alloy.…”

Section: Resultsmentioning

confidence: 99%

Atherosclerotic-Derived Endothelial Cell Response Conducted by Titanium Oxide Nanotubes

et al. 2023

View full text Add to dashboard Cite

Atherosclerosis lesions are described as the formation of an occlusive wall-vessel plaque that can exacerbate infarctions, strokes, and even death. Furthermore, atherosclerosis damages the endothelium integrity, avoiding proper regeneration after stent implantation. Therefore, we investigate the beneficial effects of TiO2 nanotubes (NTs) in promoting the initial response of detrimental human atherosclerotic-derived endothelial cells (AThEC). We synthesized and characterized NTs on Ti6Al4V by anodization. We isolated AThEC and tested the adhesion long-lasting proliferation activity, and the modulation of focal adhesions conducted on the materials. Moreover, ultrastructural cell-surface contact at the nanoscale and membrane roughness were evaluated to explain the results. Our findings depicted improved filopodia and focal adhesions stimulated by the NTs. Similarly, the NTs harbored long-lasting proliferative metabolism after 5 days, explained by overcoming cell-contact interactions at the nanoscale. Furthermore, the senescent activity detected in the AThEC could be mitigated by the modified membrane roughness and cellular stretch orchestrated by the NTs. Importantly, the NTs stimulate the initial endothelial anchorage and metabolic recovery required to regenerate the endothelial monolayer. Despite the dysfunctional status of the AThEC, our study brings new evidence for the potential application of nano-configured biomaterials for innovation in stent technologies.

show abstract

“…Various stem cells (SCs) have been widely investigated in vitro and in vivo on animal models for potential applications in dental medicine for the past two decades. SCs are defined as undifferentiated cells that can give rise to different specialized cell types or self-renew under suitable conditions [ 8 , 9 , 17 ]. Apart from well-studied mesenchymal stem cells (MSCs) from bone marrow, numerous sources of adult stem cells have been investigated, such as umbilical cord blood, muscle, adipose tissue, and teeth [ 18 , 19 ].…”

Section: Stem Cell-based Regenerative Strategies In Orthognathic Dent...mentioning

confidence: 99%

“…Adult human dental pulp stem cells were first discovered in the 21st century when researchers isolated self-renewing cells capable of forming several different cell lineages in vitro [ 7 , 8 , 9 , 17 , 18 ]. To date, a total of five stem/progenitor cells from human dental tissue have been identified ( Table 2 ).…”

Section: Stem Cell-based Regenerative Strategies In Orthognathic Dent...mentioning

confidence: 99%

“…The innovative RDM methods were targeted especially at maxillofacial defects (cleft and lip palate), acceleration of orthodontic tooth movement, and prevention of periodontal complications during orthodontic therapy. Various treatment modalities based on RDB have been described, investigated in pre-clinical settings, and proposed for clinical application in orthognathic management, including the incorporation of 3D biomimetic scaffolds (BSs) [ 5 , 6 ], advances in oral stem cells for tissue regeneration [ 4 , 7 , 8 , 9 ], biomaterials [ 10 , 11 ], growth factors (GFs) [ 7 , 12 , 13 , 14 ], and tissue-engineered pre-vascularized bone and soft tissue flaps. The complex intercellular mechanism, cells’ interplay, signaling pathways, and molecules determine the optimal regeneration of vital elements during the induced tissue-engineered reconstruction of oral and dentofacial structures.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tissue Engineering Supporting Regenerative Strategies to Enhance Clinical Orthodontics and Dentofacial Orthopaedics: A Scoping, Perspective Review

Abid

Jamal²,

Alsahafi

et al. 2023

Biomedicines

View full text Add to dashboard Cite

The personalized regenerative therapeutic strategies applicable in the structural and functional repair of maxillofacial/dental defects are expected to extend beyond the limits of what is currently possible in the management of dentofacial anomalies and treating malocclusions. The application of undifferentiated stem cells (SCs), including signaling molecule control and individualized tissue engineering based on targeted therapies, has been proposed to overcome therapeutic limitations and complications associated with treatments for craniofacial defects, including severe orthodontic discrepancies. This scoping, prospective review discusses comprehensively the current knowledge and prospects for improving clinical outcomes by the application of novel cell-required and cell-free regenerative strategies in biomedicine. The existing evidence, although scant, suggests that patients receiving an orthodontic treatment could benefit from precise tissue augmentation, allowing enhancement of tooth movement generated by orthognathic forces; faster, more predictable alignment of dental arches; optimal management of periodontal complications; and prevention of external root resorption. Ultimately, enriching orofacial tissues and “customizing” the repair of congenital/acquired defects in the craniofacial region can be vastly enhanced to provide a positive therapeutic outcome and improve patients’ quality of life.

show abstract

Cell–scaffold interactions in tissue engineering for oral and craniofacial reconstruction

Cited by 38 publications

References 402 publications

Biocompatibility Study of Hydrogel Biopolymer Scaffold with Encapsulated Mesenchymal Stem Cells

Biocompatibility Study of Hydrogel Biopolymer Scaffold with Encapsulated Mesenchymal Stem Cells

Atherosclerotic-Derived Endothelial Cell Response Conducted by Titanium Oxide Nanotubes

Tissue Engineering Supporting Regenerative Strategies to Enhance Clinical Orthodontics and Dentofacial Orthopaedics: A Scoping, Perspective Review

Contact Info

Product

Resources

About