Cell Sensing of the Physical Properties of the Microenvironment at Multiple Scales

Gautrot, Julien E.

doi:10.1002/9781118966174.ch19

Cited by 3 publications

(3 citation statements)

References 154 publications

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“…For example, when cells are cultured on a uniformly fibronectin-coated substrate, high fibronectin density is required to trigger the integrin signaling. On the other hand, if fibronectins are patterned into nanoclusters, they trigger a much higher integrin clustering, signaling, and cell function [ [81] , [82] , [83] , [84] , [85] , [86] , [87] ]. However, nano-patterning fibronectin has only be achieved on a 2D substrate to date.…”

Section: Discussionmentioning

confidence: 99%

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Jara

Wang

Prado

et al. 2020

Bioactive Materials

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Plasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1), and 10% has a heterodimeric pair of γ and more acidic γ′ chains (γγ′F1). We have synthesized a novel fibrin matrix exclusively from a 1:1 (molar ratio) complex of γγ′F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII (rFXIIIa). In this matrix, the fibrin nanofibers were decorated with pFN nanoclusters (termed γγ′F1:pFN fibrin). In contrast, fibrin made from 1:1 mixture of γγF1 and pFN formed a sporadic distribution of “pFN droplets” (termed γγF1+pFN fibrin). The γγ′F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. Three dimensional (3D) culturing showed that the γγ′F1:pFN complex fibrin matrix enhanced the proliferation of both HUVECs and primary human fibroblasts. HUVECs in the 3D γγ′F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in the γγF1+pFN fibrin. Relative to γγF1+pFN fibrin, mouse dermal wounds that were sealed by γγ′F1:pFN fibrin exhibited accelerated and enhanced healing. This study suggests that a 3D pFN presentation on a fibrin matrix promotes wound healing.

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Section: Discussionmentioning

confidence: 99%

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Jara

Wang

Prado

et al. 2020

Bioactive Materials

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“…focal adhesions (FAs) physically linking the environment via the actin cytoskeleton to the nucleus. Although intense efforts have been devoted to understand how cells sense and respond to the properties of the micro-environment via FAs, the functional underlying mechanisms are not yet fully understood [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

An automated quantitative analysis of cell, nucleus and focal adhesion morphology

2018

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Adherent cells sense the physical properties of their environment via focal adhesions. Improved understanding of how cells sense and response to their physical surroundings is aided by quantitative evaluation of focal adhesion size, number, orientation, and distribution in conjunction with the morphology of single cells and the corresponding nuclei. We developed a fast, user-friendly and automated image analysis algorithm capable of capturing and characterizing these individual components with a high level of accuracy. We demonstrate the robustness and applicability of the algorithm by quantifying morphological changes in response to a variety of environmental changes as well as manipulations of cellular components of mechanotransductions. Finally, as a proof-of-concept we use our algorithm to quantify the effect of Rho-associated kinase inhibitor Y-27632 on focal adhesion maturation. We show that a decrease in cell contractility leads to a decrease in focal adhesion size and aspect ratio.

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“…For example, when cells are cultured on a uniformly fibronectin-coated substrate, high fibronectin density is required to trigger the integrin signaling. On the other hand, if fibronectins are patterned into nanoclusters, they trigger a much higher integrin clustering, signaling, and cell function [73][74][75][76][77][78][79] . However, nano-patterning fibronectin has only be achieved on a 2D substrate to date.…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional (3D) Fibronectin Nano-Array Presented on Fibrin Matrix Accelerates Mice Skin Wound Healing

Wang

et al. 2020

Preprint

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Plasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1) and 10% has a heterodimeric pair of γ and more acidic γ' chains (γγ'F1). We have synthesized a novel fibrin matrix exclusively from a 1:1 (molar ratio) complex of γγ'F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII (rFXIIIa). In this matrix, the fibrin nanofibers were wrapped with periodic 200-300 nm wide pFN nanobands (termed γγ'F1:pFN fibrin). In contrast, fibrin made from 1:1 mixture of γγF1 and pFN formed a sporadic distribution of "pFN droplets" (termed γγF1+pFN fibrin). The γγ'F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. Three dimensional (3D) culturing showed that the γγ'F1:pFN complex fibrin matrix enhanced the proliferation of both HUVECs and primary human fibroblasts. HUVECs in the 3D γγ'F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in the γγF1+pFN fibrin. Relative to γγF1+pFN fibrin, mouse dermal wounds that were sealed by γγ'F1:pFN fibrin exhibited accelerated and enhanced healing. This study suggests that a 3D pFN nano-array presented on a fibrin matrix can promote wound healing.

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Cell Sensing of the Physical Properties of the Microenvironment at Multiple Scales

Cited by 3 publications

References 154 publications

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

Novel fibrin-fibronectin matrix accelerates mice skin wound healing

An automated quantitative analysis of cell, nucleus and focal adhesion morphology

Three-Dimensional (3D) Fibronectin Nano-Array Presented on Fibrin Matrix Accelerates Mice Skin Wound Healing

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