Cell Separation, Centrifugation

Axelsson, Hans

doi:10.1002/0471250589.ebt042

Cited by 3 publications

(5 citation statements)

References 28 publications

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“…In this technique particles or cells of different densities/volume in a suspension will settle at different rates, with the larger and denser particles settling out of suspension more rapidly based on their sedimentation velocity,

v_{d} = \frac{m g D}{k_{B} T}

; where m is mass, D is diffusion coefficient, g is the gravitation constant, k B is the Boltzmann constant, and T is the temperature (Berg, 1993). There are several different centrifugation techniques of relevance to cell sorting, including differential centrifugation, rate-zonal centrifugation, and equilibrium centrifugation, briefly described below (Axelsson, 2002). …”

Section: Overview Of Cell Separation Methodsmentioning

confidence: 99%

“…It should be noted that removal of the non-target cell population can also be achieved through other separation modalities, such as density-gradient centrifugation (Axelsson, 2002) or size-based separation (Green et al, 2009, Inglis et al, 2011, Long et al, 2008, Zhang et al, 2012a). Non-target cell depletion may be followed by a positive selection for the cell of interest (Estes et al, 2009).…”

Section: 0 Challenges and Opportunities For Further Research And Comentioning

confidence: 99%

“…Commonly, a first step for multiparameter magnetic sorting is 'debulking' of the starting cell population by using a panel of magnetic particles directed against the nontarget cells, thus depleting the population for several markers simultaneously. It should be noted that removal of the nontarget cell population can also be achieved through other separation modalities, such as density-gradient centrifugation (Axelsson 2002) or size-based separation (Long et al 2008, Green et al 2009, Inglis et al 2011, Zhang et al 2012a. Nontarget cell depletion may be followed by a positive selection for the cell of interest (Estes et al 2009).…”

Section: Cell Separation Against Multiple Markersmentioning

confidence: 99%

See 2 more Smart Citations

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

2014

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Magnetic sorting using magnetic beads has become a routine methodology for the separation of key cell populations from biological suspensions. Due to the inherent ability of magnets to provide forces at a distance, magnetic cell manipulation is now a standardized process step in numerous processes in tissue engineering, medicine, and in fundamental biological research. Herein we review the current status of magnetic particles to enable isolation and separation of cells, with a strong focus on the fundamental governing physical phenomena, properties and syntheses of magnetic particles and on current applications of magnet-based cell separation in laboratory and clinical settings. We highlight the contribution of cell separation to biomedical research and medicine and detail modern cell separation methods (both magnetic and non-magnetic). In addition to a review of the current state-of-the-art in magnet-based cell sorting, we discuss current challenges and available opportunities for further research, development and commercialization of magnetic particle-based cell separation systems.

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v_{d} = \frac{m g D}{k_{B} T}

Section: Overview Of Cell Separation Methodsmentioning

confidence: 99%

Section: 0 Challenges and Opportunities For Further Research And Comentioning

confidence: 99%

Section: Cell Separation Against Multiple Markersmentioning

confidence: 99%

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Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

2014

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“…Disk‐stack centrifuges are typically used at manufacturing‐scale because of their capacity to handle a wide variety of feedstock . They also enable processing in a continuous fashion by utilizing the functionality of a semi‐continuous solids discharge . However, most disk‐stack centrifuge designs suffer from high levels of shear being present in the feed zones.…”

Section: Introductionmentioning

confidence: 99%

An automated laboratory‐scale methodology for the generation of sheared mammalian cell culture samples

Joseph

Goldrick

Mollet³

et al. 2017

Biotechnology Journal

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Continuous disk-stack centrifugation is typically used for the removal of cells and cellular debris from mammalian cell culture broths at manufacturing-scale. The use of scale-down methods to characterize disk-stack centrifugation performance enables substantial reductions in material requirements and allows a much wider design space to be tested than is currently possible at pilotscale. The process of scaling down centrifugation has historically been challenging due to the difficulties in mimicking the Energy Dissipation Rates (EDRs) in typical machines. This paper describes an alternative and easy-to-assemble automated capillary-based methodology to generate levels of EDRs consistent with those found in a continuous disk-stack centrifuge. Variations in EDR were achieved through changes in capillary internal diameter and the flow rate of operation through the capillary. The EDRs found to match the levels of shear in the feed zone of a pilot-scale centrifuge using the experimental method developed in this paper (2.4 × 10 5 W/kg) are consistent with those obtained through previously published computational fluid dynamic studies (2.0 × 10 5 W/kg). Furthermore, this methodology can be incorporated into existing scale-down methods to model the process performance of continuous disk-stack centrifuges. This was demonstrated through the characterization of culture hold time, culture temperature and EDRs on centrate quality.

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“…Clarification can be achieved by centrifugation, tangential flow microfiltration, normal flow microfiltration, and depth filtration. High‐efficiency centrifuges have been developed for large scale bioprocessing, but these devices typically have high capital costs and there are issues involved in validation, sterilization, and aerosol generation (1–3). Microfiltration membranes, typically with 0.2 or 0.45 μm pore size, provide nearly complete retention of cells and cell debris, but rapid surface fouling can lead to low capacities and high membrane area requirements (4, 5).…”

Section: Introductionmentioning

confidence: 99%

Clarification of Yeast Cell Suspensions by Depth Filtration

Chandler¹,

Zydney²

2005

Biotechnology Progress

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Depth filtration can be very attractive for initial clarification because of low capital costs and ease of operation. However, there is currently no fundamental understanding of the effects of the filter pore size and morphology on the overall capacity and filtrate quality. The objective of this study was to examine the flux, capacity, and filtrate turbidity of a series of depth filters with different pore size ratings and multilayer structures for the filtration of yeast cell suspensions. Data were analyzed using available fouling models to obtain insights into the flux decline mechanisms. Filters with small pore size provide high filtrate quality at low capacity, with the reverse being true for the larger pore sizes. The multilayer structure of commercial depth filters leads to improved performance, although the choice of layer properties is critical. The highest capacity was achieved using a multilayer filter in which the upper layer allows significant yeast cell penetration into the filter matrix but still protects the retentive layer that is needed for a high quality filtrate.

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Cell Separation, Centrifugation

Cited by 3 publications

References 28 publications

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

An automated laboratory‐scale methodology for the generation of sheared mammalian cell culture samples

Clarification of Yeast Cell Suspensions by Depth Filtration

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