Cell shape information is transduced through tension-independent mechanisms

Ron, Ariel; Azeloglu, Evren U.; Calizo, Rhodora C.; Hu, Mufeng; Bhattacharya, Sunita; Chen, Yibang; Jayaraman, Gomathi; Lee, Sunwoo; Neves-Zaph, Susana; Li, Hong; Gordon, Ronald E.; He, John Cijiang; Hone, James; Iyengar, Ravi

doi:10.1038/s41467-017-02218-4

Cited by 57 publications

(49 citation statements)

References 46 publications

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“…In recent FRET‐based tension sensor studies , sample sizes have ranged from tens to hundreds, and statistics have been done on data that varies in length scale from subcellular structures to whole cells. Therefore, we investigated how sample size, specifically the number of cells analyzed, affects the uncertainty of FRET efficiency measurements.…”

Section: Resultsmentioning

confidence: 99%

Improving Quality, Reproducibility, and Usability of FRET‐Based Tension Sensors

et al. 2018

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Mechanobiology, the study of how mechanical forces affect cellular behavior, is an emerging field of study that has garnered broad and significant interest. Researchers are currently seeking to better understand how mechanical signals are transmitted, detected, and integrated at a subcellular level. One tool for addressing these questions is a Förster resonance energy transfer (FRET)‐based tension sensor, which enables the measurement of molecular‐scale forces across proteins based on changes in emitted light. However, the reliability and reproducibility of measurements made with these sensors has not been thoroughly examined. To address these concerns, we developed numerical methods that improve the accuracy of measurements made using sensitized emission‐based imaging. To establish that FRET‐based tension sensors are versatile tools that provide consistent measurements, we used these methods, and demonstrated that a vinculin tension sensor is unperturbed by cell fixation, permeabilization, and immunolabeling. This suggests FRET‐based tension sensors could be coupled with a variety of immuno‐fluorescent labeling techniques. Additionally, as tension sensors are frequently employed in complex biological samples where large experimental repeats may be challenging, we examined how sample size affects the uncertainty of FRET measurements. In total, this work establishes guidelines to improve FRET‐based tension sensor measurements, validate novel implementations of these sensors, and ensure that results are precise and reproducible. © 2018 International Society for Advancement of Cytometry

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Section: Resultsmentioning

confidence: 99%

Improving Quality, Reproducibility, and Usability of FRET‐Based Tension Sensors

et al. 2018

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“…In particular, cellular morphology and shapes could be one of significant parameters to determine tissue morphogenesis with structural characteristics [23]. The morphological changes of cells are critically related with nuclear shapes to regulate gene expressions in cytoskeletons and they could be the biomechanical responses to spatial microenvironments [23,31]. Therefore, nuclear deformations and orientations could be an essential factor to reflect cytoskeletal rearrangements for cell shapes or tissue-like cell collectivity organizations with the calculations of cell nuclear angulations and deformation while using NSI (or circularity) and NAR, respectively [21].…”

Section: Discussionmentioning

confidence: 99%

Prototype Development for the Periodontal Model System with the Spatial Compartmentalization by the Additive Manufacturing

Park

2019

Applied Sciences

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At present, various tissue engineering strategies have been developed for multiple tissue regeneration and integrative structure formations. However, the regenerations of tooth-supportive structures are still limited and challenging due to the micro-interfacial compartmentalization of multiple tissues, their integrations for systematic responses, and spatiotemporal organizations of engineered tissues. Here, we investigated the scaffold prototype as the regeneration platform of the periodontal complex (cementum-periodontal ligament (PDL)-bone). Based on the tooth image dataset, the prototype scaffold was designed with individual periodontal tissues while using the three-dimensional (3D) printing technique and solvent-casting method with poly-ε-caprolactone (PCL). The architecture was characterized by scanning electron microscope (SEM) and biological assessments were performed with human periodontal ligament (hPDL) cells by confocal microscope. In particular, the angulations and deformations of hPDL cells on PDL architectures were analyzed while using nuclear aspect ratio (NAR = 2.319 ± 0.273) and nuclear shape index (NSI (circularity) = 0.546 ± 0.0273). In in-vitro, designed surface microgroove patterns facilitated angular organizations of hPDL cells (frequency of 0–10° angulations = 75 ± 9.54 out of 97.3 ± 2.52) for seven days. The prototype scaffolding system showed geometric adaptation to the digitized image dataset, hPDL orientations on microgroove-patterned surface, and architectural compartmentalizations for periodontal tissue regeneration.

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“…The negative poisson's ratio structure [35] would increase the shear modulus [35] of the cell lattices. Thus the Cambrian worm epithelium is more resistant to the shear force and the shape of a cell within tissues can represent the history of physical signals it encounters [36]. In the contract and elongation stage of the ring (Figure 4f), the big octagon cells have opposite deformation direction to the small quadrangle cells [37], the anisotropy deform forces may affect cell proliferation events [38] distribution pattern within cell clusters.…”

Section: (B) Topological Structure Proprieties and Developmentmentioning

confidence: 99%

The asynchronous growth and movement reconstruction of the early molting animals

Lan

2020

Preprint

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Epithelium is one of the basic types of animal tissue, in which tissue boundaries are the physical barriers to separate adjust cell clusters. However, tissue boundary in cellular level can hardly found in the fossil records. Here I focus on the growth and movement patterns of the early Ecdysozoa being through quantifying forces in cellular level of the epithelium of two worms from the early Cambrian and Ordovician period. The epithelium cells (might be but not necessarily represent the biological cell) separate adjust body ring of the early Ecdysozoa indicates a precise morphological patterning in cellular level has been established in the early Cambrian. The forces distribution patterns on the body ring suggest the boundary cells are contribute to maintain pressure gradient among rings which are the building blocks of the movement pattern of the worm. The active tension field of the worms plates though ontogenetic trajectory while epithelium cells are in the steady state indicate these molting animals employ asynchronous growth pattern of the epithelium.

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Cell shape information is transduced through tension-independent mechanisms

Cited by 57 publications

References 46 publications

Improving Quality, Reproducibility, and Usability of FRET‐Based Tension Sensors

Improving Quality, Reproducibility, and Usability of FRET‐Based Tension Sensors

Prototype Development for the Periodontal Model System with the Spatial Compartmentalization by the Additive Manufacturing

The asynchronous growth and movement reconstruction of the early molting animals

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