Cell Sheet-Based Tissue Engineering for Fabricating 3-Dimensional Heart Tissues

Shimizu, Tatsuya

doi:10.1253/circj.cj-14-0973

Cited by 42 publications

(31 citation statements)

References 64 publications

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“…hMSCs are primary cells with the capability to self-renewal and to differentiate into osteogenic, adipogenic and chondrogenic lineages [4,5]. By combining hMSCs with 3D scaffolds made of different biomaterials, tissue engineering is able to provide tissue-specific in vitro 3D models which mimic the in vivo environment for studies in the fields of regenerative medicine, human diseases, new therapies development and diagnostics [6,7]. In clinical applications, MSCs, in combination with other factors (e.g., growth factors, mechanical and chemical properties of the biomaterial itself), will both stimulate and initiate the tissue repair process by differentiating into specific cell lineage or by secreting factors which in turn stimulate other specialized cells to repair the damaged tissue [3,8].…”

mentioning

confidence: 99%

A First Approach to Evaluate the Cell Dose in Highly Porous Scaffolds By Using a Nondestructive Metabolic Method

Divieto

Sassi

2015

Future Sci. OA

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Background:In cell-based therapies, in vitro studies on biomimetic cell–scaffold constructs can facilitate the determination of the cell dose, a key factor in guaranteeing the effectiveness of the treatment. However, highly porous scaffolds do not allow a nondestructive evaluation of the cell number. Our objective was to develop a nondestructive method for human mesenchymal stem cells dose evaluation in a highly porous scaffold for bone regeneration.Materials & measurement method:Proliferation trend of human mesenchymal stem cells on Biocoral® scaffolds was measured by a resazurin-based assay here optimized for 3D cultures. The method allows to noninvasively follow the cell proliferation on biocorals over 3 weeks with very high reproducibility.Conclusion:This reliable method could be a powerful tool in cell-based therapies for cell dose determination.

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confidence: 99%

A First Approach to Evaluate the Cell Dose in Highly Porous Scaffolds By Using a Nondestructive Metabolic Method

Divieto

Sassi

2015

Future Sci. OA

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“…76 With this technique, entire sheets of monolayered iPSC-CMs can be detached from the culture surface, fully preserving cell surface and extracellular components, and raising the possibility of fabricating cardiovascular tissues by overlaying multiple sheets. 77,78 Another interesting approach was developed in Murry’s laboratory, 79 that involves spontaneous aggregation of hESC-CM during suspension culture on a rotating orbital shaker. Cells aggregated forming macroscopic disc-shaped patches of beating tissue with a 300–600 μm thickness.…”

Section: Introductionmentioning

confidence: 99%

“The state of the heart”: Recent advances in engineering human cardiac tissue from pluripotent stem cells

Sirabella

Cimetta

Vunjak-Novakovic

2015

Exp Biol Med (Maywood)

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The pressing need for effective cell therapy for the heart has led to the investigation of suitable cell sources for tissue replacement. In recent years, human pluripotent stem cell research expanded tremendously, in particular since the derivation of human induced pluripotent stem cells. In parallel, bioengineering technologies have led to novel approaches for in vitro cell culture. The combination of these two fields holds potential for in vitro generation of high-fidelity heart tissue, both for basic research and for therapeutic applications. However, this new multidisciplinary science is still at an early stage. Many questions need to be answered and improvements need to be made before clinical applications become a reality. Here we discuss the current status of human stem cell differentiation into cardiomyocytes and the combined use of bioengineering approaches for cardiac tissue formation and maturation in developmental studies, disease modeling, drug testing and regenerative medicine.

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“…This work was pioneered by tissue engineering researchers more than a decade ago . The engineered heart tissue can be a complex 3‐D construct, or simply in a form of cell sheet(s) . The cell sheet can be directly patched (transplanted) on to the surface of the heart, or may be further engineered into desired 3‐D constructs.…”

mentioning

confidence: 99%

“…The cell sheet can be directly patched (transplanted) on to the surface of the heart, or may be further engineered into desired 3‐D constructs. Different types of cells and their combinations have been used to produce cardiac patches, either in conjunction with various types of scaffold materials, or in a scaffold‐free format . Cells that have been employed for this purpose include primary neonatal cardiomyocytes, fibroblasts, adipose‐derived stromal cells, induced pluripotent stem cells, mesenchymal stem cells, and cardiac‐derived progenitor cells .…”

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confidence: 99%

Search for magic patches that heal the broken heart

Jiang

2016

Clin Exp Pharma Physio

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Heart failure caused by acute myocardial infarction remains a great challenge in clinical practice due to the high mortality rate associated with this pathology. Current therapies mainly aim to prevent expansion of the initial infarct lesion; however, approaches to effective regeneration of functional myocardium remain elusive. A recent breakthrough is that scientists have shown a collagen-based cardiac patch containing follistatin-like 1 can not only attenuate infarction-induced heart injuries, but also stimulate proliferation of endogenous myocytes. These results raise the possibility that the intrinsic cardiomyocyte division, albeit a rare phenomenon, may be therapeutically manipulated and augmented for treating myocardial infarction and heart failure, at least in mice.

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Cell Sheet-Based Tissue Engineering for Fabricating 3-Dimensional Heart Tissues

Cited by 42 publications

References 64 publications

A First Approach to Evaluate the Cell Dose in Highly Porous Scaffolds By Using a Nondestructive Metabolic Method

A First Approach to Evaluate the Cell Dose in Highly Porous Scaffolds By Using a Nondestructive Metabolic Method

“The state of the heart”: Recent advances in engineering human cardiac tissue from pluripotent stem cells

Search for magic patches that heal the broken heart

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