Cell Sheet Tissue Engineering for Heart Failure

Sekine, Hidekazu; Shimizu, Tatsuya; Okano, Teruo

doi:10.1007/978-4-431-54628-3_3

Cited by 12 publications

(8 citation statements)

References 21 publications

(19 reference statements)

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“…Cell sheet technology is a scaffold-free method where thermosensitive substrates produce single cells and complete layers of cells for tissue regeneration. This cardiac tissue engineering approach may provide better heart models in the long-term ( Sekine et al, 2016 ; Kobayashi et al, 2019 ; Zurina et al, 2020 ). The technique generally uses specific culture dishes, coated with temperature-sensitive polymers such as poly(N-isopropylacrylamide).…”

Section: Preserving or Rebuilding Cardiovascular Structures For Modelmentioning

confidence: 99%

New Modalities of 3D Pluripotent Stem Cell-Based Assays in Cardiovascular Toxicity

et al. 2021

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The substantial progress of the human induced pluripotent stem cell (hiPSC) technologies over the last decade has provided us with new opportunities for cardiovascular drug discovery, regenerative medicine, and disease modeling. The combination of hiPSC with 3D culture techniques offers numerous advantages for generating and studying physiological and pathophysiological cardiac models. Cells grown in 3D can overcome many limitations of 2D cell cultures and animal models. Furthermore, it enables the investigation in an architecturally appropriate, complex cellular environment in vitro. Yet, generation and study of cardiac organoids—which may contain versatile cardiovascular cell types differentiated from hiPSC—remain a challenge. The large-scale and high-throughput applications require accurate and standardised models with highly automated processes in culturing, imaging and data collection. Besides the compound spatial structure of organoids, their biological processes also possess different temporal dynamics which require other methods and technologies to detect them. In this review, we summarise the possibilities and challenges of acquiring relevant information from 3D cardiovascular models. We focus on the opportunities during different time-scale processes in dynamic pharmacological experiments and discuss the putative steps toward one-size-fits-all assays.

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Section: Preserving or Rebuilding Cardiovascular Structures For Modelmentioning

confidence: 99%

New Modalities of 3D Pluripotent Stem Cell-Based Assays in Cardiovascular Toxicity

et al. 2021

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“…Additionally, the retainment of biological ECM on the basal side of the cell sheet can act as an adhesive agent, allowing the culture surfaces to be directly attached to the host tissue without the use of fibrin glue or a suture. This unique characteristic has shown to enhance cell engraftment and retention [20,25]. Numerous studies have shown that cell sheet technology can improve LV function, increase neovascularization, and decrease fibrosis and remodeling in MI models [4,16,25,26].…”

Section: Cell Sheet Technologymentioning

confidence: 99%

“…This unique characteristic has shown to enhance cell engraftment and retention [20,25]. Numerous studies have shown that cell sheet technology can improve LV function, increase neovascularization, and decrease fibrosis and remodeling in MI models [4,16,25,26]. Cell sheet technology appears to be one of the most promising novel therapeutic techniques as it has the potential to overcome the major obstacles presented with cell injection and bioscaffold engineering.…”

Section: Cell Sheet Technologymentioning

confidence: 99%

Cell Therapy And Methods Of Stem Cell Delivery For Regeneration Of Heart Tissue Following Myocardial Infarction

Spence¹,

Gallicchio²

2021

Journal of Stem Cell Research

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Myocardial infarction (MI) results in irreversible loss of cardiomyocytes (CMs) and can often lead to heart failure. Due to the minimal regeneration capacity of the myocardium, novel therapeutic techniques are needed. Cell therapy has emerged as a promising treatment for MI and involves the introduction of stem cells into the infarct area where they can proliferate and regenerate functional heart tissue. These cells can be delivered by various methods, including direct injection, scaffold formation, and cell sheet preparation. Of these, cell sheet technology appears to hold the most promise as it allows for maximal cell engraftment and retention without significant harmful side effects. Furthermore, the best composition of the cell sheet has since been debated. Cell sheets composed of skeletal myoblasts (SMs), mesenchymal cells (MSCs), and pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), have proven to be of the greatest interest for engraftment into infarcted myocardium. Due to their vast differentiation and proliferation potential, pluripotent stem cells show a unique ability to readily regenerate functional myocardium following transplantation. iPSCs express the same pluripotency and induce similar effects in vivo as human ESCs, while circumventing certain sourcing problems and controversies, making them a favorable alternative Identification of an effective combination cell delivery method that allows for prolonged improvement in heart function while decreasing rates of cell death would represent a major advancement in stem cell therapy and the clinical treatment of MI.

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“…On the other hand, as an advantageous cell-based therapy for HF, tissue-engineered cell sheet technology has been developed for effective delivery of regenerative cells [ 17 , 18 ], such as murine adipose cells [ 19 ], human iPSCs [ 20 ], and EPCs [ 21 ] into cardiac tissues damaged by ischemia or myocarditis. We have also demonstrated that fibroblast sheets (F-sheet) co-cultured with EPCs recover cardiac function in a rat myocardial infarction model [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model

Sekine

Kawaguchi

Miyazawa

et al. 2022

Biology

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Fulminant myocarditis causes impaired cardiac function, leading to poor prognosis and heart failure. Cell sheet engineering is an effective therapeutic option for improving cardiac function. Naïve blood mononuclear cells (MNCs) have been previously shown to enhance the quality and quantity of cellular fractions (QQMNCs) with anti-inflammatory and vasculogenic potential using the one culture system. Herein, we investigated whether autologous cell sheet transplant with QQMNCs improves cardiac function in a rat model with experimental autoimmune myocarditis (EAM). Fibroblast sheets (F-sheet), prepared from EAM rats, were co-cultured with or without QQMNCs (QQ+F sheet) on temperature-responsive dishes. QQ+F sheet induced higher expression of anti-inflammatory and vasculogenic genes (Vegf-b, Hgf, Il-10, and Mrc1/Cd206) than the F sheet. EAM rats were transplanted with either QQ+F sheet or F-sheet, and the left ventricular (LV) hemodynamic analysis was performed using cardiac catheterization. Among the three groups (QQ+F sheet, F-sheet, operation control), the QQ+F sheet transplant group showed alleviation of end-diastolic pressure–volume relationship on a volume load to the same level as that in the healthy group. Histological analysis revealed that QQ+F sheet transplantation promoted revascularization and mitigated fibrosis by limiting LV remodeling. Therefore, autologous QQMNC-modified F-sheets may be a beneficial therapeutic option for EAM.

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Cell Sheet Tissue Engineering for Heart Failure

Cited by 12 publications

References 21 publications

New Modalities of 3D Pluripotent Stem Cell-Based Assays in Cardiovascular Toxicity

New Modalities of 3D Pluripotent Stem Cell-Based Assays in Cardiovascular Toxicity

Cell Therapy And Methods Of Stem Cell Delivery For Regeneration Of Heart Tissue Following Myocardial Infarction

Transplantation of Fibroblast Sheets with Blood Mononuclear Cell Culture Exerts Cardioprotective Effects by Enhancing Anti-Inflammation and Vasculogenic Potential in Rat Experimental Autoimmune Myocarditis Model

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