Cell Signaling Experiments Driven by Optical Manipulation

Difato, Francesco; Pinato, Giulietta; Cojoc, D.

doi:10.3390/ijms14058963

Cited by 16 publications

(9 citation statements)

References 125 publications

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“…The variance of the QPD signal decreases when the adhesion contact is stabilized through the binding to a receptor, or by receptors clustering at the bead contact. The lowand high-frequency components of the bead motion are prevalently influenced by actin polymerization 26,27 and myosin II motor contraction 28 .…”

Section: Resultsmentioning

confidence: 99%

HDAC6 and RhoA are novel players in Abeta-driven disruption of neuronal polarity

et al. 2015

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Maintenance of neuronal polarity and regulation of cytoskeletal dynamics are vital during development and to uphold synaptic activity in neuronal networks. Here we show that soluble b-amyloid (Ab) disrupts actin and microtubule (MT) dynamics via activation of RhoA and inhibition of histone deacetylase 6 (HDAC6) in cultured hippocampal neurons. The contact of Ab with the extracellular membrane promotes RhoA activation, leading to growth cone collapse and neurite retraction, which might be responsible for hampered neuronal pathfinding and migration in Alzheimer's disease (AD). The inhibition of HDAC6 by Ab increases the level of heterodimeric acetylated tubulin and acetylated tau, both of which have been found altered in AD. We also find that the loss of HDAC6 activity perturbs the integrity of axon initial segment (AIS), resulting in mislocalization of ankyrin G and increased MT instability in the AIS concomitant with loss of polarized localization of tau and impairment of action potential firing.

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Section: Resultsmentioning

confidence: 99%

HDAC6 and RhoA are novel players in Abeta-driven disruption of neuronal polarity

et al. 2015

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“…The force feedback can reduce the time of operations and increase the feasible complexity of tasks. The new capabilities offered by haptic optical tweezers will inspire study and diagnosis of new types of cells 105,106 or allow complex assembly of biomedical materials. 107 …”

Section: Discussionmentioning

confidence: 99%

Invited Article: A review of haptic optical tweezers for an interactive microworld exploration

Pacoret

Régnier²

2013

Review of Scientific Instruments

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This paper is the first review of haptic optical tweezers, a new technique which associates force feedback teleoperation with optical tweezers. This technique allows users to explore the microworld by sensing and exerting picoNewton-scale forces with trapped microspheres. Haptic optical tweezers also allow improved dexterity of micromanipulation and micro-assembly. One of the challenges of this technique is to sense and magnify picoNewton-scale forces by a factor of 10(12) to enable human operators to perceive interactions that they have never experienced before, such as adhesion phenomena, extremely low inertia, and high frequency dynamics of extremely small objects. The design of optical tweezers for high quality haptic feedback is challenging, given the requirements for very high sensitivity and dynamic stability. The concept, design process, and specification of optical tweezers reviewed here are focused on those intended for haptic teleoperation. In this paper, two new specific designs as well as the current state-of-the-art are presented. Moreover, the remaining important issues are identified for further developments. The initial results obtained are promising and demonstrate that optical tweezers have a significant potential for haptic exploration of the microworld. Haptic optical tweezers will become an invaluable tool for force feedback micromanipulation of biological samples and nano- and micro-assembly parts.

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“…Our proposed ways and the idea of increasing the number of binding regions with a single optical set-up may be a very helpful tool for almost all kinds of controlled manipulations, for fully immersed multiple objects. We believe that this work will have an impact on various real world applications such as to extract unwanted biological cells, control atmospheric pollution, fractionate blood particles [48], cell signaling research [49], or controlled manipulation of DNA and live cells [35,50], in an innocuous manner.…”

Section: Resultsmentioning

confidence: 99%

“…We believe our theoretical work will be a very helpful guide to the highest number of feasible manipulation schemes with a single setup, especially for biological and other real life applications [48][49][50].…”

Section: Introductionmentioning

confidence: 99%

Tractor beam for fully immersed multiple objects: Long distance pulling, trapping, and rotation with a single optical set‐up

et al. 2015

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In this article, we have theoretically demonstrated the mechanism of an active tractor beam for multiple fully immersed objects with additional abilities to yielding stable long distance levitation, a controlled rotation and a desired 3D trapping. This is demonstrated with a single optical setup by using two coaxial, or even non-coaxial, superimposed higher order monochromatic Bessel beams of reverse helical nature and different frequencies. The superimposed beams can possess periodic intensity variations both along and around the beam-axis due to a difference in longitudinal wave-numbers and beam orders, respectively. The difference in frequencies of the two laser beams makes the intensity pattern to move along and around the beam-axis in a continuous way without manual ramping of phase, which allows for bidirectional movement of completely immersed multiple particles. The condition for increasing or decreasing the dimension of binding regions is also proposed here to manipulate multiple immersed objects of different sizes under dipole approximation.

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Cell Signaling Experiments Driven by Optical Manipulation

Cited by 16 publications

References 125 publications

HDAC6 and RhoA are novel players in Abeta-driven disruption of neuronal polarity

HDAC6 and RhoA are novel players in Abeta-driven disruption of neuronal polarity

Invited Article: A review of haptic optical tweezers for an interactive microworld exploration

Tractor beam for fully immersed multiple objects: Long distance pulling, trapping, and rotation with a single optical set‐up

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