Cell size controlled in plants using DNA content as an internal scale

Dario, Marco Di; Tavares, Rafael Garcia; Schiessl, Katharina; Desvoyes, Bénédicte; Gutiérrez, Crisanto; Howard, Martin; Sablowski, Robert

doi:10.1126/science.abb4348

Cited by 91 publications

(91 citation statements)

References 41 publications

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“…They demonstrated that, upon replicative stress, WEE1 interacts with and phosphorylates the F-box protein FBL17 inducing its ubiquitylation and further 26S proteasome degradation [123]. In line with its previously described role in KRP degradation [68][69][70]125,126], the loss of FBL17 proteins results in KRP accumulation causing CDK/CYC inhibition, and therefore a blockage in S phase progression [123]. As the p21 and p27 CDK inhibitors are targeted by the mammal Skp2 [127,128] and given that the human Wee1A is able to target Skp2 in vitro, even if these data need further explorations, it is tempting to believe that the mechanistic WEE1-FBL17/Skp2-CKIs-CDKs pathway might be conserved between plants and animals to trigger cell cycle arrest [123].…”

Section: Wee1 a Key Intra-s Checkpoint Proteinsupporting

confidence: 62%

Connections between the Cell Cycle and the DNA Damage Response in Plants

Gentric

Genschik

Noir

2021

IJMS

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Due to their sessile lifestyle, plants are especially exposed to various stresses, including genotoxic stress, which results in altered genome integrity. Upon the detection of DNA damage, distinct cellular responses lead to cell cycle arrest and the induction of DNA repair mechanisms. Interestingly, it has been shown that some cell cycle regulators are not only required for meristem activity and plant development but are also key to cope with the occurrence of DNA lesions. In this review, we first summarize some important regulatory steps of the plant cell cycle and present a brief overview of the DNA damage response (DDR) mechanisms. Then, the role played by some cell cycle regulators at the interface between the cell cycle and DNA damage responses is discussed more specifically.

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Section: Wee1 a Key Intra-s Checkpoint Proteinsupporting

confidence: 62%

Connections between the Cell Cycle and the DNA Damage Response in Plants

Gentric

Genschik

Noir

2021

IJMS

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“…This hypothesis has been verified in yeast and mammalian systems 45 , 46 . In Arabidopsis, recent studies show segregation of set amounts of chromatin-bound KRP4 CDK inhibitors act as a cell size sensor that is diluted by cell growth and is responsible for cell size-dependent cell cycle regulation at G1/S 47 .…”

Section: Discussionmentioning

confidence: 99%

A hierarchical transcriptional network activates specific CDK inhibitors that regulate G2 to control cell size and number in Arabidopsis

et al. 2022

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How cell size and number are determined during organ development remains a fundamental question in cell biology. Here, we identified a GRAS family transcription factor, called SCARECROW-LIKE28 (SCL28), with a critical role in determining cell size in Arabidopsis. SCL28 is part of a transcriptional regulatory network downstream of the central MYB3Rs that regulate G2 to M phase cell cycle transition. We show that SCL28 forms a dimer with the AP2-type transcription factor, AtSMOS1, which defines the specificity for promoter binding and directly activates transcription of a specific set of SIAMESE-RELATED (SMR) family genes, encoding plant-specific inhibitors of cyclin-dependent kinases and thus inhibiting cell cycle progression at G2 and promoting the onset of endoreplication. Through this dose-dependent regulation of SMR transcription, SCL28 quantitatively sets the balance between cell size and number without dramatically changing final organ size. We propose that this hierarchical transcriptional network constitutes a cell cycle regulatory mechanism that allows to adjust cell size and number to attain robust organ growth.

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“…Another study reported that a cell cycle regulator, KIP‐related protein 4, uses DNA content as an internal scale to regulate cell size in the Arabidopsis shoot stem cell niche (D'Ario et al. 2021). That the epidermal cell size is unchanged when comparing 5B to 0B suggests the transcriptome size of mRNA does not increase with more Bs.…”

Section: Discussionmentioning

confidence: 99%

Effect of aneuploidy of a non‐essential chromosome on gene expression in maize

et al. 2022

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The non-essential supernumerary maize (Zea mays) B chromosome (B) has recently been shown to contain active genes and to be capable of impacting gene expression of the A chromosomes. However, the effect of the B chromosome on gene expression is still unclear. In addition, it is unknown whether the accumulation of the B chromosome has a cumulative effect on gene expression. To examine these questions, the global expression of genes, microRNAs (miRNAs), and transposable elements (TEs) of leaf tissue of maize W22 plants with 0-7 copies of the B chromosome was studied. All experimental genotypes with B chromosomes displayed a trend of upregulated gene expression for a subset of A-located genes compared to the control. Over 3000 A-located genes are significantly differentially expressed in all experimental genotypes with the B chromosome relative to the control. Modulations of these genes are largely determined by the presence rather than the copy number of the B chromosome. By contrast, the expression of most B-located genes is positively correlated with B copy number, showing a proportional gene dosage effect. The B chromosome also causes increased expression of A-located miRNAs. Differentially expressed miRNAs potentially regulate their targets in a cascade of effects. Furthermore, the varied copy number of the B chromosome leads to the differential expression of A-located and B-located TEs. The findings provide novel insights into the function and properties of the B chromosome.

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Cell size controlled in plants using DNA content as an internal scale

Cited by 91 publications

References 41 publications

Connections between the Cell Cycle and the DNA Damage Response in Plants

Connections between the Cell Cycle and the DNA Damage Response in Plants

A hierarchical transcriptional network activates specific CDK inhibitors that regulate G2 to control cell size and number in Arabidopsis

Effect of aneuploidy of a non‐essential chromosome on gene expression in maize

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