Cell size dynamics and viability of cells exposed to hypotonic treatment and electroporation for electrofusion optimization

Ušaj, Marko; Trontelj, Katja; Hudej, Rosana; Kandušer, Maša; Miklavčič, Damijan

doi:10.2478/v10019-009-0017-9

Cited by 40 publications

(23 citation statements)

References 45 publications

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“…On the other hand, the decreases of the cell volume, which are detected predominantly at highest nystatin concentrations after their maximum has been reached and are reflected in the “type C” behavior pattern (Fig 3b), are explained as a consequence of irreversible slow cell-membrane ruptures (“type-II tension-pore behavior”). Similar cell changes were observed also in different cell lines in a hypotonic buffer [44]. …”

Section: Discussionsupporting

confidence: 80%

Osmotic Effects Induced by Pore-Forming Agent Nystatin: From Lipid Vesicles to the Cell

et al. 2016

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The responses of Chinese hamster ovary epithelial cells, caused by the pore-forming agent nystatin, were investigated using brightfield and fluorescence microscopy. Different phenomena, i.e., the detachment of cells, the formation of blebs, the occurrence of “cell-vesicles” and cell ruptures, were observed. These phenomena were compared to those discovered in giant lipid vesicles. A theoretical model, based on the osmotic effects that occur due to the size-discriminating nystatin transmembrane pores in lipid vesicles, was extended with a term that considers the conservation of the electric charge density in order to describe the cell’s behavior. The increase of the cellular volume was predicted and correlated with the observed phenomena.

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Section: Discussionsupporting

confidence: 80%

Osmotic Effects Induced by Pore-Forming Agent Nystatin: From Lipid Vesicles to the Cell

et al. 2016

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“…The results are development of interstitial edema, increased IFP, and decreased intravascular pressure, which all contribute to compromised blood flow in a way similar to that seen after the application of chemical VDAs [77]. It should be noted that swelling of cells after EP with similar dynamics as seen for endothelial cells was also observed in some other types of cells in vitro [87].…”

Section: Blood Flow-modifying Effects Of Epmentioning

confidence: 84%

Antivascular effects of electrochemotherapy: implications in treatment of bleeding metastases

Jarm

Čemažar

Miklavčič

et al. 2010

Expert Review of Anticancer Therapy

198

132

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“…Likewise, we do not exclude active volume regulation by studied cells, but did not observe any effects that would need to be explained by it. In addition, earlier studies noted suppression of such regulation in electroporated cells [54], making their response more predictable from the physico-chemical point of view.…”

Section: Discussionmentioning

confidence: 99%

Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulses

Nesin

Pakhomova

Xiao

et al. 2011

Biochimica et Biophysica Acta (BBA) - Biomembranes

157

132

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Intense nanosecond-duration electric pulses (nsEP) open stable nanopores in cell plasma membrane, followed by cell volume changesdue to water uptake or expulsion, as regulated by the osmolality balance of pore-impermeable solutes inside and outside the cell. The size of pores opened by 50, 60-ns EP (10 Hz, ~13 kV/cm) and 5, 600-ns EP (1 Hz, ~6 kV/cm) in GH3 cells was estimated by isoosmotic replacement of bath NaCl with (polyethylene glycols and sugars. Such replacement reduced cell swelling and/or turned it into a transient or sustained shrinking, depending on the availability of pores permeable to the test solute. Unexpectedly, solute substitutions showed that for the same integral area of pores opened by 60- and 600-ns treatments (as indicated by cell volume changes), the pore sizes were similar. However, the 600-ns exposure triggered significantly higher cell uptake of propidium. We concluded that 600-ns EP opened a greater number of larger (propidium-permeable pores), but the fraction of the larger pores in the entire pore population was insufficient to contribute to cell volume changes. For both the 60- and 600-ns exposures, cell volume changes were determined by pores smaller than 0.9 nm in diameter; however, the diameter increased with increasing the nsEP intensity.

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Cell size dynamics and viability of cells exposed to hypotonic treatment and electroporation for electrofusion optimization

Abstract: Background. Various electrofusion parameters have to be adjusted to obtain the optimal electrofusion ef

Cited by 40 publications

References 45 publications

Osmotic Effects Induced by Pore-Forming Agent Nystatin: From Lipid Vesicles to the Cell

Osmotic Effects Induced by Pore-Forming Agent Nystatin: From Lipid Vesicles to the Cell

Antivascular effects of electrochemotherapy: implications in treatment of bleeding metastases

Manipulation of cell volume and membrane pore comparison following single cell permeabilization with 60- and 600-ns electric pulses

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