Cell specialization in collecting tubules of the guinea pig kidney: Carbonic anhydrase activity and glycosaminoglycan production in different cells

Sato, Akira; Spicer, Samuel S.

doi:10.1002/ar.1092020402

Cited by 27 publications

(12 citation statements)

References 24 publications

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“…Moreover, LeHir et al (1982) have recently demonstrated with an ultrastructural, preembedment method, that binding of PNA in the collecting tubule of the rabbit kidney is restricted to the luminal surface of intercalated cells; whereas the principal cells are unstained. The cell types in collecting ducts of the guinea pig kidney have been further characterized by the localization of carbonic anhydrase exclusivelv in the intercalated cells (Sato and Spicer, 1982). The principal cells differed further in containing chondroitin sulfate-rich cytoplasmic granules and possessing a surface coat with chondroitin sulfate in contrast to the neutral glycoprotein comprising the intercalated cell's glycocalyx.…”

Section: Discussionmentioning

confidence: 99%

Histochemical evaluation of mouse and rat kidneys with lectin‐horseradish peroxidase conjugates

1983

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Paraffin sections of mouse and rat kidney were stained with a battery of ten lectin-horseradish peroxidase conjugates and lectin binding was correlated with the ultrastructural distribution of periodate-reactive sugar residues as determined by the periodic acid-thiocarbohydrazide-silver proteinate technique. Various segments of the uriniferous tubule in both species showed differential affinity for labelled lectins. Significant differences were also evident between comparable tubular segments in mouse and rat kidneys. Neutral glycoconjugates containing terminal beta-galactose and terminal alpha-N-acetylgalactosamine were prevalent on the luminal surface of the proximal convoluted tubule in the rat, but alpha-N-acetylgalactosamine was absent in this site in the mouse. In both species, terminal N-acetylglucosamine was abundant in the brush border of proximal straight tubules but absent in proximal convolutions. Fucose was demonstrated in both proximal and distal segments of mouse kidney tubules but only in the distal nephron and collecting ducts in the rat. Lectin staining revealed striking heterogeneity in the structure and distribution of cellular glycoconjugates. Such cellular heterogeneity was previously unrecognizable with earlier histochemical methods. The marked cellular heterogeneity observed with several lectin-conjugates in distal convoluted tubules and collecting ducts of both species raises a prospect that lectins can provide specific markers for intercalated and principal cells in the mammalian kidney. Glycoconjugates containing terminal sialic acid and penultimate beta-galactose were present on vascular endothelium in both rodent kidneys, as were terminal alpha-galactose residues; but both species lacked reactivity for Ulex europeus I lectin in contrast to human vascular endothelial cells. The constant binding pattern of lectin conjugates allows convenient and precise differentiation of renal tubular segments and should prove valuable in the study of changes in kidney morphology promoted by experimental manipulation or pathologic changes.

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Section: Discussionmentioning

confidence: 99%

Histochemical evaluation of mouse and rat kidneys with lectin‐horseradish peroxidase conjugates

1983

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“…The antibodies against affinity purified CA I1 isoenzyme were raised in rabbits as described earlier in detail (15). The purity and specificity testing of these antibodies have been documented extensively (4,16). Rabbit anti-(Na+K)-ATPase antiserum used was a generous gift from Dr. G. Siegel (Department of Neurology, University of Michigan, Ann Arbor, MI) and was raised in rabbits against a mouse brain (Na+K)-ATPase catalytic unit as described earlier in detail (8).…”

Section: Methodsmentioning

confidence: 99%

Ontogeny of Cell Type-Specific Enzyme Reactivities in Kidney Collecting Ducts

Holthöfer

1987

Pediatr Res

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ABSTRACT. Highly specific antibodies against vital enzymes of the collecting ducts were used to study the appearance of cell type specific enzyme profiles in developing rat kidneys. (Na+K)-ATPase, the abundant enzyme of principal cells, could be detected early in utero in most collecting duct cells. However, the characteristic basolatera1 polarization of this enzyme did not appear until the first hours after birth. After this, the relative amount of (Na+K)-ATPase immunoreactive cells along collecting ducts decreased steadily, to reach the amount found in adult rat kidneys by the 30th postnatal day. Carbonic anhydrase immunoreactivity characteristic for intercalated cells was not detectable in fetal kidneys, but appeared soon after birth, with steadily increasing numbers of cells that were positive. Interestingly, immunoreactive band 3 glycoprotein (anion channel protein of erythrocytes) did not appear until the 5th day of life, with only a slowly increasing number of cells positive for this probe. These results, showing the sequential appearance of cell type-specific enzyme reactivities along collecting ducts, likely reflect a similar pattern of functional development of the respective main cell types. These results may provide an explanation for physiologic neonatal acidosis, as the enzyme profile associated with proton secretion was seen to appear slowly during the first weeks of life in a distinct manner. (Pediatv Res 22: [504][505][506][507][508] 1987)

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“…The luminal surface of the distal nephron has a glycocalyx composed of glycosaminoglycans as well as other proteins that are set in a hydrated polysaccharide gel (43,44,61,65,96,97,109,126). Glycosaminoglycans consist of negatively charged disaccharide repeats that are linked to core proteins.…”

Section: Sensing Flow In Principal and Intercalated Cellsmentioning

confidence: 99%

Regulation of cation transport in the distal nephron by mechanical forces

Satlin¹,

Carattino²,

Liu³

et al. 2006

American Journal of Physiology-Renal Physiology

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Thiazide and loop diuretics induce renal K(+) secretion, often leading to renal K(+) wasting and hypokalemia. This phenomenon has been proposed to reflect an increase in delivery to and reabsorption of Na(+) by the distal nephron, with a resultant increase in the driving force for passive K(+) efflux across the apical membrane. Recent studies suggest that cellular mechanisms that lead to enhanced rates of Na(+) reabsorption as well as K(+) secretion in response to increases tubular flow rates are more complex. Increases in tubular flow rates directly enhance the activity of apical membrane Na(+) channels and indirectly activate a class of K(+) channels, referred to as maxi-K, that are functionally inactive under low flow states. This review addresses the role of biomechanical forces, generated by variations in urinary flow rate and tubular fluid volume, in the regulation of transepithelial Na(+) and K(+) transport in the distal nephron. The question of why the distal nephron has evolved to include a component of flow-dependent K(+) secretion is also addressed.

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Cell specialization in collecting tubules of the guinea pig kidney: Carbonic anhydrase activity and glycosaminoglycan production in different cells

Cited by 27 publications

References 24 publications

Histochemical evaluation of mouse and rat kidneys with lectin‐horseradish peroxidase conjugates

Histochemical evaluation of mouse and rat kidneys with lectin‐horseradish peroxidase conjugates

Ontogeny of Cell Type-Specific Enzyme Reactivities in Kidney Collecting Ducts

Regulation of cation transport in the distal nephron by mechanical forces

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