Cell-specific Processing of Chromogranin A in Endocrine Cells of the Rat Stomach

Norlén, Per; Curry, William; Björkqvist, Maria; Maule, Aaron G.; Cunningham, Rodat T.; Hogg, Robert B.; Harriott, Pat; Johnston, C.F.; Hutton, John C.; Håkanson, R.

doi:10.1177/002215540104900102

Cited by 39 publications

(22 citation statements)

References 43 publications

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“…These results differed from those reported by Norlén et al (2001), who found most CgA antigens in this cell type in the rat stomach. This disparity can be attributed to species differences in the expression of CgA or somatostatin.…”

Section: Discussioncontrasting

confidence: 99%

“…Curry et al (1991) found different immunoreactive patterns with their antibodies at various levels of the rat GI tract but did not relate the staining results to the cell type. Norlén et al (1997Norlén et al ( ,2001) reported a similar study but referred their staining results to the different NE cell types in the rat stomach. No corresponding immunocytochemical study has been reported in the human GI tract.…”

mentioning

confidence: 84%

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Chromogranin A in the Human Gastrointestinal Tract: An Immunocytochemical Study with Region-specific Antibodies

Portela-Gomes

Stridsberg

2002

J Histochem Cytochem.

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S U M M A R YWe studied the immunoreactivity of 12 different region-specific antibodies to the chromogranin A (CgA) molecule in the various neuroendocrine cell types of the human gastrointestinal (GI) tract by using double immunofluorescence techniques. These staining results were compared with others obtained with a commercial monoclonal CgA antibody (LK2H10). G (gastrin)-cells showed immunoreactivity to virtually all region-specific antibodies, but with varying frequency. Most intestinal EC (enterochromaffin)-and L (enteroglucagon)-cells were immunoreactive to the antibodies to the N-terminal and mid-portion of the CgA molecule, whereas the EC-cells in the stomach reacted with fewer region-specific antibodies. D (somatostatin)-cells reacted to the CgA 411-424 antibody and only occasionally showed immunoreactivity to the other CgA antibodies. A larger cytoplasmic area was stained with the antibodies to than with the other antibodies tested. These differences in staining pattern may reflect different cleavage of the CgA molecule in different cell types and at different regions of the GI tract.

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Section: Discussioncontrasting

confidence: 99%

mentioning

confidence: 84%

Chromogranin A in the Human Gastrointestinal Tract: An Immunocytochemical Study with Region-specific Antibodies

Portela-Gomes

Stridsberg

2002

J Histochem Cytochem.

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“…The N-terminal WE-14 antiserum (R814.1) consistently immunostained a larger population of cells relative to that detected by the C-terminal WE-14 antiserum; this would suggest that the antiserum exhibits a degree of crossreaction with CgA or larger processed intermediates. The observations in this study are consistent with previous immunohistological characterization of the CgA and WE-14 antisera (Curry et al 1991;Norlen et al 2000).…”

Section: Discussionsupporting

confidence: 93%

Immunohistochemical localization of WE-14 in the developing porcine sympathoadrenal cell lineage

Barkatullah

Pogue

Depreitere

et al. 2001

Histochem Cell Biol

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Immunohistochemical investigation of the post-translational processing of chromogranin A (CgA) to generate WE-14 in the sympathoadrenal cell lineage of the developing porcine fetus (F) detected intense CgA and weak WE-14 immunoreactivity in migrating neuroblast cells of the diffuse sympathetic ganglia adjacent to the dorsal aorta and projecting toward the cortical mass at F24-27. F37-42; WE-14 immunoreactivity was detected in chromaffinoblasts at the periphery of the developing cortex and at F54-56 days gestation WE-14 immunoreactivity was detected in a large population of central medullary cells. From F74 to F76 days and thereafter the number of cells exhibiting intense WE-14 immunostaining decreased, and the majority of chromaffin cells exhibited uniform weak WE-14 immunostaining. At postnatal day 1 (P1) intense WE-14 immunoreactivity was primarily confined to clusters of chromaffin cells with weak immunostaining in the general population. The transitory neuroblasts, chromaffinoblasts, and maturing chromaffin cell population exhibited uniform intense CgA immunostaining through gestation and after birth. Additional observations detected intense CgA and WE-14 immunostaining in extrachromaffin tissue at P1 and in neuronal-like cells in vessels of the aortic arch at F37. This study has demonstrated that CgA is post-translationally processed to generate WE-14 during early fetal development in the migrating progenitor cells of the porcine sympathoadrenal lineage.

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“…All major endocrine cell types express Chromogranin A in the stomach (Norlen et al 2001). As shown in Figure 5, B and C, there were fewer Chromogranin A-positive cells in the ngn3 −/− stomach than in the control.…”

Section: Gastric Enteroendocrine Differentiation Is Impaired In Ngn3mentioning

confidence: 82%

Neurogenin 3 is essential for the proper specification of gastric enteroendocrine cells and the maintenance of gastric epithelial cell identity

Lee¹,

Perreault²,

Brestelli³

et al. 2002

Genes Dev.

273

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The notch signaling pathway is essential for the endocrine cell fate in various tissues including the enteroendocrine system of the gastrointestinal tract. Enteroendocrine cells are one of the four major cell types found in the gastric epithelium of the glandular stomach. To understand the molecular basis of enteroendocrine cell development, we have used gene targeting in mouse embryonic stem cells to derive an EGFP-marked null allele of the bHLH transcription factor, neurogenin 3 (ngn3). In ngn3 −/− mice, glucagon secreting A-cells, somatostatin secreting D-cells, and gastrin secreting G-cells are absent from the epithelium of the glandular stomach, whereas the number of serotonin-expressing enterochromaffin (EC) cells is decreased dramatically. In addition, ngn3 −/− mice display intestinal metaplasia of the gastric epithelium. Thus, ngn3 is required for the differentiation of enteroendocrine cells in the stomach and the maintenance of gastric epithelial cell identity.

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Cell-specific Processing of Chromogranin A in Endocrine Cells of the Rat Stomach

Cited by 39 publications

References 43 publications

Chromogranin A in the Human Gastrointestinal Tract: An Immunocytochemical Study with Region-specific Antibodies

Chromogranin A in the Human Gastrointestinal Tract: An Immunocytochemical Study with Region-specific Antibodies

Immunohistochemical localization of WE-14 in the developing porcine sympathoadrenal cell lineage

Neurogenin 3 is essential for the proper specification of gastric enteroendocrine cells and the maintenance of gastric epithelial cell identity

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