Cell stiffness measurement using two-fingered microhand

Kawakami, Daiki; Ohara, Kenichi; Takubo, Tomohito; Mae, Yasushi; Ichikawa, Akihiko; Tanikawa, Tamio; Arai, Tatsuo

doi:10.1109/robio.2010.5723466

Cited by 17 publications

(5 citation statements)

References 17 publications

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“…We prepared the spheroid culture chip as previously described. 33 Polydimethylsiloxane (PDMS) (Silpot 184, Dow Corning Toray, Co., Ltd., Japan) was used for fabrication of 3D culture chips (Fig. 1A and B).…”

Section: Fabrication Of 3d Culture Devicesmentioning

confidence: 99%

“…Detailed system information has been previously described. 33,34 In this study, the load applied to an end-effector was measured when it was pushed in a spheroid, and the measured load (reaction force of the spheroid) was used as an index of hardness. The two-ngered microhand rst grasped a spheroid and then the microhand prepared the spheroid for loading by pressing it 20 mm.…”

Section: Hardness Measurement Of Spheroidmentioning

confidence: 99%

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A 3D culture model study monitoring differentiation of dental epithelial cells into ameloblast-like cells

et al. 2016

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Section: Fabrication Of 3d Culture Devicesmentioning

confidence: 99%

Section: Hardness Measurement Of Spheroidmentioning

confidence: 99%

A 3D culture model study monitoring differentiation of dental epithelial cells into ameloblast-like cells

et al. 2016

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“…Therefore, the inclination angle of the tip of the end-effector is 18°, and the strength of this structure is analyzed. Based on a previous study, the reactive force of the cell on the end-effector is 4,000 nN [31]. The results of a finite element stress analysis of a force of 4,000 nN applied to the tip of the end-effector are shown in figure 2.…”

Section: Fabrication Process and Conditionsmentioning

confidence: 99%

Development of an optimum end-effector with a nano-scale uneven surface for non-adhesion cell manipulation using a micro-manipulator

Horade

Kojima

Kamiyama

et al. 2015

J. Micromech. Microeng.

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In order to realize effective micro-manipulation using a micro-manipulator system, an optimum end-effector is proposed. Cell-manipulation experiments using mouse fibroblast cells are conducted, and the usability of the proposed end-effector is confirmed. A key advantage of the micro-manipulator is high-accuracy, high-speed 3D micro- and nano-scale positioning. Micro-manipulation has often been used in research involving biological cells. However, there are two important concerns with the micro-manipulator system: gripping efficiency and the release of gripped objects. When it is not possible to grip a micro-object, such as a cell, near its center, the object may be dropped during manipulation. Since the acquisition of exact position information for a micro-object in the vertical direction is difficult using a microscope, the gripping efficiency of the end-effector should be improved. Therefore, technical skill or operational support is required. Since, on the micro-scale, surface forces such as the adsorption force are greater than body forces, such as the gravitational force, the adhesion force between the end-effector and the object is strong. Therefore, manipulation techniques without adhesion are required for placed an object at an arbitrary position. In the present study, we consider direct physical contact between the end-effector and objects. First, the design and materials of the end-effector for micro-scale manipulation were optimized, and an end-effector with an optimum shape to increase the grip force was fabricated. Second, the surface of the end-effector tip was made uneven, and the adhesion force from increasing on the micro-scale was prevented. When an end-effector with an uneven surface was used, release without adhesion was successful 85.0% of the time. On the other hand, when an end-effector without an uneven surface was used, release without adhesion was successful 6.25% of the time. Therefore, the superiority of a structure with an uneven surface was demonstrated, and high-accuracy cell manipulation without sliding during handling and without adhesion was performed successfully.

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“…There are also high expectations for treating individual cell characteristics as biomarkers, focusing on their correlation with the disease. In a previous study, a method was reported in which a micromanipulator and strain gauge for directly measuring hardness were integrated, and a force sensor was used to measure the hardness directly [29]. When using a micromanipulator, throughput is low because it is difficult to measure multiple cells simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Cell size and deformation measurement using constrictions integrated into a microfluidic device

Horade,

Moriga,

Murakami

2024

Phys. Scr.

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In this study, we developed a microfluidic device to evaluate cell size and deformability. As a specific method, a physical pushing load was applied to the cells using a channel having a constriction with a height of 1 μm. In addition, by orienting the constriction in a vertical direction, it was possible to measure the cell area easily using a microscope under load. The system constructed in this study can evaluate the contact area between the cells and the glass surface before and after applying a load under a microscope. The only input parameter was the syringe flow rate, and it was possible to evaluate multiple cells in a cell suspension simultaneously. Also, since the flow rate is 50 μm/min or less, there is no need for a high-speed camera. This time, we evaluated cell types with different characteristics: NIH/3T3 and smooth muscle cells (SMC). To evaluate deformability, we focused on the circularity of the cells during load application. Due to the influence of the flow within the channel, cells with high deformability assumed an almost elliptical shape and flowed through the constriction. Using the device developed in this study, we confirmed that SMCs, which are muscle cells, have large variations in cell size and hardness among individual cells. Finally, we discussed these results and possible future applications.

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Cell stiffness measurement using two-fingered microhand

Cited by 17 publications

References 17 publications

A 3D culture model study monitoring differentiation of dental epithelial cells into ameloblast-like cells

A 3D culture model study monitoring differentiation of dental epithelial cells into ameloblast-like cells

Development of an optimum end-effector with a nano-scale uneven surface for non-adhesion cell manipulation using a micro-manipulator

Cell size and deformation measurement using constrictions integrated into a microfluidic device

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