Cell Surface Antigenic Characteristics of Rat Large Granular Lymphocytes (Lgl)

Reynolds, Craig W.; Ortaldo, John R.; Denn, Alfred C.; Barlozzari, Teresa; Herberman, Ronald B.; Sharrow, Susan O.; Ramsey, Keith M.; Okumura, Ko; Habu, Sonoko

doi:10.1016/b978-0-12-341360-4.50027-5

Cited by 7 publications

(6 citation statements)

References 12 publications

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“…The treatment with LeuOMe furthermore showed that the alloreactive cells were LGL; a morphology for practical purposes associated with NK cells since very few, if any, freshly isolated blood or spleen LGL from normal rats stain with mAb directed towards CD4 or CD5 [44,50].The demonstration that pure populations of IL2-activated NK cells devoid of B and Tcells [47] also exhibited alloreactivity, further confirmed our conclusion.…”

supporting

confidence: 76%

“…Only about 5% of nude spleen cells were CD3+, CD5+ or TcR a/@+ (Table 1) making it highly unlikely that these T cells were responsible for the observed spontaneous alloreactivity in v i m . On the other hand, a large proportion (51%-57%) of spleen cells from nudes stained with antibodies known to react with NK cells like OX34 (CD2), OX8 (CDS), 3.2.3 (NKR-P1) and OX52 [39,44,451 (Table 1). In contrast, about 70% of normal spleen cells expressed the Tcell markers CD3, CD5 and TcR alp, 82%-83% of the cells were CD2+ and OX52+,whereas 18% stained with the 3.2.3 mAb ( Table 1).…”

Section: Phenotypic Characterization Of Nude Spleen Cellsmentioning

confidence: 99%

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Allospecific recognition of hemic cells in vitro by natural killer cells from athymic rats: Evidence that allodeterminants coded for by single major histocompatibility complex haplotypes are recognized

et al. 1991

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We have previously shown that large granular lymphocyte (LGL)-enriched cell populations have the capacity to spontaneously recognize and kill allogeneic small lymphocytes and bone marrow cells (BMC) in vitro in certain strain combinations of rats. Here, we have studied the alloreactivity of natural killer (NK) cells from PVG nude (RT1c) rats against a panel of major histocompatibility complex (MHC) incompatible hemic cells. Both lymphocytes and BMC from the AO (RT1u), DA (RT1a), BN (RT1n) as well as the MHC-congenic PVG-RT1u (RT1u) rat strains were efficiently killed in vitro, whereas cells from syngeneic PVG rats were spared. The structures recognized on lymphocytes and BMC were probably similar since the two cell populations inhibited each other in cross-competition experiments. A number of features aligned the alloreactive effector cells with NK cells and not T cells. (a) Only about 5% of the effector cells from nude spleens expressed the T cell antigens CD3, CD5 or T cell receptor (TcR) alpha/beta whereas greater than 50% of the cells expressed markers present on NK cells (CD2, CD8, OX52 and the rat NK cell-specific marker NKR-P1 recognized by the monoclonal antibody 3.2.3). (b) The alloreactive cells were granular since pretreatment of nude spleen cells with the lysosomotropic agent L-leucine methyl ester which eliminated LGL, simultaneously abolished the cytolysis of both allogeneic lymphocytes and YAC-1 tumor cells. (c) Nude spleen cells stimulated with human recombinant interleukin 2 for 1 week in vitro generated large granular proliferating cells which were CD3-, CD5-, TcR alpha/beta-, but greater than 95% 3.2.3+. These cells efficiently killed allogeneic hemic cells from the same rat strains as did freshly isolated effector cells. (d) The cytolysis of allogeneic hemic cells could effectively be inhibited with unlabelled NK-sensitive (YAC-1 and K-562), but not NK-resistant (Roser leukemia) tumor cells. Cross-competition studies showed that PVG nude NK cells discriminated between AO, BN and DA BMC, suggesting that different alloantigens were positively recognized by subsets of NK cells. The mode of inheritance of the allodeterminant specifically recognized on AO BMC was investigated in crosses and backcrosses between AO and BN or DA rats. A gene dosage effect was observed in that this determinant was expressed at a slightly reduced level in F1 hybrids.(ABSTRACT TRUNCATED AT 250 WORDS)

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supporting

confidence: 76%

Section: Phenotypic Characterization Of Nude Spleen Cellsmentioning

confidence: 99%

Allospecific recognition of hemic cells in vitro by natural killer cells from athymic rats: Evidence that allodeterminants coded for by single major histocompatibility complex haplotypes are recognized

et al. 1991

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“…The OX-19' cells were morphologically recognized as small, dense, agranular lymphocytes from which we have shown in a previous report that they don't have NK activity [ 11. Although all LGL showed the OX-8+/OX-19-phenotype, an important subpopulation was asialo-GM1-which means that the LGL were heterogeneous in the expression of the latter NK marker. Heterogeneity of NK cells has been repeatedly reported [15,161 and it has been proposed that in the rat and human most, if not all, NK cells are LGL but not all LGL have NK activity [2,17,181. Interestingly, in the present study, the asialo-GMl' and asialo-GM1-LGL subpopulations were seen to differ morphologically, namely in the diameter of their granules.…”

Section: Discussionmentioning

confidence: 98%

Immuno‐electron microscopic characterization of large granular lymphocytes (natural killer cells) from rat liver

Bouwens¹,

Wisse

1987

Eur J Immunol

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The immunocytochemical characteristics of large granular lymphocytes (LGL), isolated from the liver sinusoids of euthymic and athymic (nude) rats, were investigated in electron microscopy by the immunoperoxidase technique. The LGL were found positive for MRC OX-8 (natural killer cells and cytotoxic/suppressor T cells) and negative for MRC OX-19 (pan-T marker) in both rat strains. The LGL were heterogeneous in the expression of the natural killer cell marker asialo-GM1 which was found on 56% of the LGL from euthymic and on 71% of the LGL from athymic rats. LGL were easily distinguished from the other cells in the preparations, "conventional" lymphocytes and monocytes, thanks to their highly characteristic ultrastructural features, in particular by the presence of specific electron-dense cytoplasmic granules and rod-cored vesicles. These features have been described formerly for the so-called "pit cells" and are more reliable than the classical LGL characteristics at the light microscopic level, i.e. the presence of azurophilic granules. Our results give further support for the existence of an important population of natural killer cells in the liver sinusoids.

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“…2). Antibody W3/13 recognizes a cell surface antigen that is present not only on T cells, but also on large granular lymphocytes and polymorphonuclear cells (10). Because of its broader spectrum, W3/13 does not reveal the T cell lymphopenia as well as the Fluorescence histograms on two IC rats, one with and one without T cell lymphopenia, using monoclonal antibodies recognizing different T cell subsets.…”

Section: Resultsmentioning

confidence: 99%

Two genes required for diabetes in BB rats. Evidence from cyclical intercrosses and backcrosses.

Jackson¹,

Buse²,

Rifai³

et al. 1984

The Journal of Experimental Medicine

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The BB rat strain spontaneously develops diabetes mellitus which rapidly progresses to ketoacidosis and death if not treated with exogenous insulin. 4 0 -60% of BB rats develop overt diabetes and exhibit prominent insulitis, selective pancreatic beta cell destruction, and circulating antibodies to islet cell surfaces (1, 2). Diabetes can be prevented with multiple forms of immunotherapy and can be transferred with activated splenic iymphocytes (3, 4). In addition to the diabetic syndrome, BB rats have a striking lymphophenia characterized by an almost total lack of circulating T cells (5). Colleet al. (6-8), using a sequential breeding program, have described associations between the development of diabetes and the inheritance of major histocompatibility complex (MHC) 1 genes, lymphopenia, and susceptibility to pancreatic lymphocytic infiltrates.One of us (R. M. W.) initiated a breeding program, different from that of Dr. Coile and co-workers, to isolate the BB rat's diabetogenic genes on the genetic background of three inbred rat strains. BB rats were cyclically backcrossedintercrossed then backcrossed with Brown Norway (BN), Lewis (L), and WistarFurth (WF) rats while selecting for diabetes. We studied the expression of lymphopenia and MHC antigens. We will present evidence that at least two independent genes or gene complexes are absolutely necessary for the inheritance of diabetes in these rats. One gene (l), determines the T cell lymphopenia of the BB rat and is not linked to the rat MHC. The second gene (RTI-DM), determines the susceptibility to diabetes and is closely linked to the rat MHC. Materials a n d M e t h o d sBreeding Program. Outbred diabetic BB rats and the animals used in this study were maintained at

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Cell Surface Antigenic Characteristics of Rat Large Granular Lymphocytes (Lgl)

Cited by 7 publications

References 12 publications

Allospecific recognition of hemic cells in vitro by natural killer cells from athymic rats: Evidence that allodeterminants coded for by single major histocompatibility complex haplotypes are recognized

Allospecific recognition of hemic cells in vitro by natural killer cells from athymic rats: Evidence that allodeterminants coded for by single major histocompatibility complex haplotypes are recognized

Immuno‐electron microscopic characterization of large granular lymphocytes (natural killer cells) from rat liver

Two genes required for diabetes in BB rats. Evidence from cyclical intercrosses and backcrosses.

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