Cell surface binding of adenosine to Dictyostelium and inhibition of pulsatile signalling

Newell, P. C.

doi:10.1016/0378-1097(82)90202-6

Cited by 10 publications

(21 citation statements)

References 3 publications

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“…It would thus seem likely that adenosine acts indirectly by binding to receptor molecules other than the cyclic AMP receptors. This conclusion is supported by another study demonstrating rapid, reversible binding of 3H-labelled adenosine to adenosine-specific cell surface receptors present at 7 x lo6 molecules per cell (Newell, 1982). The lack of any effect on the activity of the cell-bound phosphodiesterase enzyme indicates that these adenosine receptors are not the active sites of the plasma membrane-bound phosphodiesterase.…”

Section: Discussionmentioning

confidence: 74%

Inhibition by Adenosine of Aggregation Centre Initiation and Cyclic AMP Binding in Dictyostelium

Newell

Ross

1982

Microbiology

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The inhibitory action of adenosine on the initiation of aggregation centre formation in starving Dictyostelium discoidem was examined and used to test assumptions of theoretical models of cyclic AMP signalling. Adenosine at 5 mM strongly inhibited aggregation centre initiation without preventing signal relay. Inosine or AMP at 10 mM had no effect and did not prevent the effect of adenosine. Adenosine had no effect on cellular phosphodiesterase activity, contact site A formation, or chemotaxis to small drops of cyclic AMP. The chemotactically-related responses of rapid cyclic GMP formation and changes in light scattering after pulses of cyclic AMP did not show any inhibition or decrease in sensitivity to the signal in the presence of adenosine. However, binding of 3H-labelled cyclic AMP to cell surface receptors was strongly inhibited by adenosine. The maximum inhibition was approximately 90% and was non-competitive with respect to cyclic AMP. The role of cell surface cyclic AMP receptors in the mechanism of initiation and the implications of the effects of adenosine for the role of cyclic GMP as a secondary messenger are discussed.

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Section: Discussionmentioning

confidence: 74%

Inhibition by Adenosine of Aggregation Centre Initiation and Cyclic AMP Binding in Dictyostelium

Newell

Ross

1982

Microbiology

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“…In spite of the structural similarity, adenosine does not compete with cAMP for the cAMP surface receptors [26,19]. Instead, adenosine acts non-competitively and indirectly though β-receptors as one of the cellular adenosine binding sides [26,30,18]. Alterations in the ribose moiety of adenosine in most cases increase the inhibitory effect on cAMP binding [18].…”

Section: Discussionmentioning

confidence: 97%

“…Adenosine reduces oscillatory cAMP activity [26,19,27] and plays a role in cell type regulation at later states of the developmental cycle [28,29]. In spite of the structural similarity, adenosine does not compete with cAMP for the cAMP surface receptors [26,19]. Instead, adenosine acts non-competitively and indirectly though β-receptors as one of the cellular adenosine binding sides [26,30,18].…”

Section: Discussionmentioning

confidence: 99%

“…Adenosine is a physiological degradation product of cAMP and has been shown to inhibit several cAMPdepending processes by inhibition of cAMP binding [19] and postaggregative differentiation [18]. Adenosine reduces oscillatory cAMP activity [26,19,27] and plays a role in cell type regulation at later states of the developmental cycle [28,29]. In spite of the structural similarity, adenosine does not compete with cAMP for the cAMP surface receptors [26,19].…”

Section: Discussionmentioning

confidence: 99%

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Streamless aggregation of Dictyostelium in the presence of isopropylidenadenosin

Hilgardt

Čejková

Hauser

et al. 2008

Biophysical Chemistry

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“…The prediction of function from a given nucleic acid or amino acid sequence requires precise and unambiguous knowledge 8 Protein Linguistics and the Modular Code of the Cytoskeleton 191 of the functions that are attributed to similar sequences [27,72,74] or threedimensional folds [21,41]. Currently, the ambiguity of annotations in databases is hindering progress in this direction.…”

Section: Protein Linguisticsmentioning

confidence: 99%

The Codes of Life

Barbieri¹,

Hoffmeyer²

2008

Biosemiotics

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Aims and Scope of the SeriesCombining research approaches from biology, philosophy and linguistics, the emerging field of biosemiotics proposes that animals, plants and single cells all engage in semiosis -the conversion of physical signals into conventional signs. This has important implications and applications for issues ranging from natural selection to animal behaviour and human psychology, leaving biosemiotics at the cutting edge of the research on the fundamentals of life.The Springer book series Biosemiotics draws together contributions from leading players in international biosemiotics, producing an unparalleled series that will appeal to all those interested in the origins and evolution of life, including molecular and evolutionary biologists, ecologists, anthropologists, psychologists, philosophers and historians of science, linguists, semioticians and researchers in artificial life, information theory and communication technology.

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Cell surface binding of adenosine to Dictyostelium and inhibition of pulsatile signalling

Cited by 10 publications

References 3 publications

Inhibition by Adenosine of Aggregation Centre Initiation and Cyclic AMP Binding in Dictyostelium

Inhibition by Adenosine of Aggregation Centre Initiation and Cyclic AMP Binding in Dictyostelium

Streamless aggregation of Dictyostelium in the presence of isopropylidenadenosin

The Codes of Life

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