Cell Surface Changes during Electromagnetic Field Exposure

Hamada, Spencer H.; Witkus, R.; Griffith, R.D.

doi:10.1159/000163501

Cited by 9 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The research, which focused on cell shape and cell surface modifications, provided evidence for time related changes in shape, microvilli (number and shape), surface sugar residues and cytoskeleton organization. Modifications of cell shape and plasma membranes as a consequence of exposure to MFs or EMFs in other cell lines have also been reported recently [Hamada et al, 1989;Paradisi et al, 1993;Santoro et al, 1997;Lisi et al, 2000;Chionna et al, 2003;Rieti et al, 2004]. This indicates that morphological modifications may be considered a Fig.…”

Section: Discussionsupporting

confidence: 60%

Time dependent modifications of Hep G2 cells during exposure to static magnetic fields

Chionna¹,

Tenuzzo²,

Panzarini³

et al. 2005

Bioelectromagnetics

View full text Add to dashboard Cite

Morphological modifications, i.e., cell shape, cell surface sugar residues, cytoskeleton, and apoptosis of Hep G2 cells during 24 h exposure to 6 mT static magnetic field (static MF) were studied by means of light and electron microscopy and cytochemistry. Progressive modifications of cell shape and surface were observed during the entire period of exposure to static MF. Control cells were polyhedric with short microvilli covering the cell surface, while those exposed to static MF, were elongated with many irregular microvilli randomly distributed on the cell surface. At the end of the exposure period, the cells had a less flat shape due to partial detachment from the culture dishes. However, throughout the period of exposure under investigation, the morphology of the organelles remained unmodified and cell proliferation was only partially affected. In parallel with cell shape changes, the microfilaments and microtubules, as well as the quantity and distribution of surface ConA-FITC and Ricinus communnis-FITC labeling sites, were modified in a time dependent manner. Apoptosis, which was almost negligible at the beginning of experiment, increased to about 20% after 24 h of continuous exposure. The induction of apoptosis was likely due to the increment of [Ca2+]i during exposure. In conclusion, the data reported in the present work indicates that 6 mT static MF exposure exerts time dependent biological effects on Hep G2 cells.

show abstract

Section: Discussionsupporting

confidence: 60%

Time dependent modifications of Hep G2 cells during exposure to static magnetic fields

Chionna¹,

Tenuzzo²,

Panzarini³

et al. 2005

Bioelectromagnetics

View full text Add to dashboard Cite

show abstract

“…It is generally known that morphological and structural changes to the plasma membrane interfere with many functional and structural features of the cells, leading, for example, to changes in cellular shape, cytoskeleton arrangement, ion flux, receptor distribution, phagocytosis etc. Modifications in cell shape and plasma membrane as a consequence of exposure to MFs or EMFs have also been reported in many different cells (Hamada et al, 1989;Paradisi et al, 1993;Santoro et al, 1997;Lisi et al, 2000;Chionna et al, 2003;Rieti et al, 2004). Moderate-intensity (6 mT) SMFs exert a strong and replicable effect on cell shape and plasma membrane of different cell types (Chionna et al, 2003, in press).…”

Section: Plasma Membrane Cell Shape and Cytoskeletonmentioning

confidence: 97%

Bioeffects of moderate-intensity static magnetic fields on cell cultures

Dini

Abbro

2005

Micron

258

148

View full text Add to dashboard Cite

“…Cambios semejantes han sido reportados con el uso de diversos campos magnéticos, pulsantes o constantes, en cultivos celulares tanto en células adherentes como no adherentes (8,9,10,11,12). En nuestro estudio, sin embargo, no se apreciaron cambios morfológicos en las células no adherentes K-562 (eritroleucemia humana) Los cambios que han sido descritos por la exposición a campos magnéticos moderados (6mT) consisten, para el caso de células que crecen en suspensión, en la pérdida de su forma redondeada y en la adquisición de una alargada e irregular, mientras que para las células adherentes se trata de modificaciones en su forma y orientación o en la pérdida de su adherencia, quedando, de esta manera, libremente suspendidas en el cultivo (13).…”

Section: Discussionunclassified

Efecto De Los Campos Magnéticos Pulsantes Sobre Líneas De Células Tumorales McF-7 Y K-562

Pinilla¹,

Pombo²,

Urueña³

et al. 2014

rev cuarzo

View full text Add to dashboard Cite

RESUMENNumerosas investigaciones sugieren que los campos magné-ticos inducen apoptosis en diversas líneas tumorales, aparentemente sin afectar a células sanas. Sin embargo, las conclusiones son heterogéneas debido a las diferencias en las características de los campos magnéticos utilizados. Nuestro estudio se realizó con el objetivo de determinar el efecto de los campos magnéticos pulsantes sobre la morfología, el recuento celular y la viabilidad en líneas tumorales MCF-7 y K-562. Las células fueron expuestas a campos pulsantes positivos , de 6 mT y de 60 Hz, por periodos de una o de tres horas . El efecto se comparó con el producido por Doxorrubicina 0,075 µM o 10 µM. En este artículo se exponen los principales resultados de dicho estudio.Palabras clave: cáncer, medicina alternativa, terapéuticas alternativas, viabilidad celular, apoptosis, campos magnéticos pulsantes. ABSTRACTNumerous studies suggest that magnetic fields induce apoptosis in various tumor cell lines, apparently without affecting healthy cells. However, the conclusions are heterogeneous due to the differences in the characteristics of magnetic fields used. Our study was conducted to determine the effect of pulsed magnetic fields on the morphology, cell count and viability in tumor cell lines MCF-7 and K-562. Cells were exposed to positive pulsed field of 6 mT and 60 Hz, for periods of one or three hours. The effect was compared with that produced by doxorubicin 0.075 µM to 10 µM. This article presents the main results of the study are presented.

show abstract

Cell Surface Changes during Electromagnetic Field Exposure

Cited by 9 publications

References 10 publications

Time dependent modifications of Hep G2 cells during exposure to static magnetic fields

Time dependent modifications of Hep G2 cells during exposure to static magnetic fields

Bioeffects of moderate-intensity static magnetic fields on cell cultures

Efecto De Los Campos Magnéticos Pulsantes Sobre Líneas De Células Tumorales McF-7 Y K-562

Contact Info

Product

Resources

About