Cell surface expression of activating receptors and co-receptors on peripheral blood NK cells in systemic autoimmune diseases

Puxeddu, Ilaria; Bongiorni, F.; Chimenti, Daniele; Bombardieri, Stefano; Moretta, Alessandro; Bottino, Cristina; Migliorini, Paola

doi:10.3109/03009742.2011.648657

Cited by 35 publications

(41 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With regard to NKp30 expression, our data are therefore consistent with those previously reported in a small series of patients with systemic sclerosis (24), another autoimmune disease also characterized by interstitial lung disease, a manifestation shared with aSS.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome

Hervier

Perez

Allenbach

et al. 2016

The Journal of Immunology

View full text Add to dashboard Cite

Antisynthetase syndrome (aSS) is characterized by the association of interstitial lung disease and myositis with anti–tRNA synthetase autoantibodies. Immune mechanisms leading to aSS could be initiated in the lungs, but the role of NK cells has not yet been studied. Both extensive NK cell phenotype and functions were compared between 33 patients and 26 controls. Direct and redirected polyfunctionality assays (degranulation and intracellular production of TNF-α and IFN-γ) were performed spontaneously or after IL-12 plus IL-18 stimulation in the presence of K562 or P815 target cells, respectively. NK cells from inactive patients showed normal phenotype, whereas active aSS revealed a differentiated NK cell profile, as indicated by increased CD57 and Ig-like transcript 2 and an inability to produce IFN-γ (p = 0.002) compared with controls. Importantly, active aSS was more specifically associated with a significant NKp30 decrease (p = 0.009), although levels of mRNA and intracellular protein were similar in aSS and healthy controls. This NKp30 decrease was strongly correlated with reduced NK cell polyfunctionality in both direct and redirected killing assays with anti-NKp30 Abs (p = 0.009 and p = 0.03, respectively), confirming its important impact in aSS. Histological studies revealed massive infiltrations of NK cells inside the lungs of aSS patients (148 versus 11/mm2). Taken together, these data suggest that NK cells and NKp30 could play a role in aSS pathogenesis.

show abstract

Section: Discussionsupporting

confidence: 92%

“…With the exception of aSS, involvement of NK cells has been observed in various inflammatory and autoimmune diseases (13,14,(21)(22)(23), including those characterized by pulmonary manifestations (24). Importantly, note that NK cells have been attributed both a harmful and a beneficial role (21,24).…”

Section: Discussionmentioning

confidence: 99%

Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome

Hervier

Perez

Allenbach

et al. 2016

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…A study by Hervier et al [46] reported an increase in the frequency of NKG2C-positive cells in SLE patients, whereas Ye et al [48] found a decrease. The frequency of NKG2D-positive cells was lower in SLE patients in 2 studies (Puxeddu et al [47] and Li et al [50]), whereas Hervier et al [46], Schepis et al [49], and Ye et al [48] found no substantial differences. The same can be said of the frequency of NK cells positive for the activating receptor 2B4 (increased in 1 study; unchanged in another) or the cytotoxicity receptor NKp46 (increased in SLE patients 2 two studies; unchanged in another 2) [46][47][48][49].…”

Section: Nk Cells In Sle-perennial Underachievers?mentioning

confidence: 89%

“…Studies of various, typical NK-specific receptors in PBMCs, such as NKp30, NKp80, NKp44, and LAIR-1, showed no variations between healthy individuals and SLE patients or between SLE patients with active or inactive disease [46][47][48][49]. A decrease in the frequency of NK cells positive for the inhibitory receptor NKG2A, the KIR family receptors 2DL1/DS1, 2DL3, and 3DL1, and the activating receptor DNAM-1 (CD226) was found in SLE patients compared with healthy individuals [46][47][48]50]. NK cells in patients with active disease also express higher levels of the activating receptor CD69 [46].…”

Section: Nk Cells In Sle-perennial Underachievers?mentioning

confidence: 99%

Recent findings on the role of natural killer cells in the pathogenesis of systemic lupus erythematosus

Spada

Rojas

Barber

2015

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Systemic lupus erythematosus is a chronic, multifactorial autoimmune disease of complex etiology, characterized by loss of tolerance to nuclear autoantigens, expansion of autoreactive T and B cell clones, polyclonal B cell activation that gives rise to hypergammaglobulinemia, and increased autoantibody production, as well as immune complex deposition and multiorgan tissue inflammation. As disease progresses, immune cells, mainly T cells and macrophages, infiltrate affected organs and amplify the local inflammatory response. Natural killer cells are large, granular lymphocytes that are an important link between the innate and adaptive immune systems; variations in their activity correlate with several autoimmune diseases. To date, the literature has disregarded natural killer cells as relevant modulators in systemic lupus erythematosus pathogenesis, as these cells are few in number and show a dysfunctional phenotype in patients with active systemic lupus erythematosus. This review focuses on research that could help define the role of natural killer cells in systemic lupus erythematosus and their function in regulating this autoimmune disorder in nonlymphoid organs.

show abstract

“…With high dimensionality and wide dynamic range capability, this technique permits single cell characterization of various immune subsets and detection of intracellular phosphoproteins (Wu et al, 2010), transcription factors (Crellin et al, 2007) and cytokines (Foster et al, 2007). It has become an important tool widely used in clinical settings such as for the diagnosis of leukemia (Stetler-Stevenson and Tembhare, 2011), immunological disorders (Puxeddu et al, 2012) and monitoring of drug therapy (Galligan et al, 2009).…”

Section: Introductionmentioning

confidence: 99%