Cell Surface Hydrophobicity of <i>Actinomyces pyogenes</i> Determined by Hexadecane Adherence‐ and Salt Aggregation Studies

Ding, H.; Lämmler, Christoph

doi:10.1111/j.1439-0450.1992.tb01148.x

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…3A). 일반적으로 Pseudonocardia를 포함 하는 많은 방선균들이 고체배지 위에서 집락(colony)을 이룰 경 우 기균사(aerial hyphae) 및 포자(spore)들은 보통 소수성 (hydrophobicity)을 나타내는 것으로 알려져 있다 (Clark et al, 1985;Ding and Lammler, 1992). (Fig.…”

Section: )와 가장 가까운 근연관계가 있음을 확인할 수 있었다(16sunclassified

A Unique Prokaryotic Assemblage of Wall Biofilm of a Volcanic Cave (Daesubee) in Jeju

Moon¹,

Jung²,

Kim³

et al. 2013

The Korean Journal of Microbiology

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Cave environment provides special ecosystems for evolution of lives distant from surface environments. We investigated bacterial and archaeal communities of wall biofilm obtained from of a volcanic cave (Daesubee) in Jeju, Republic of Korea. Bacterial and archaeal 16S rRNA genes were PCR-amplified and sequenced using pyrosequencing technologies. Unique prokaryotic communities with low diversities were observed. The main bacterial sequences (ca. 83% of total reads) were affiliated with Pseudonocardia mongoliensis of phylum Actinobacteria and clustered with clones obtained from various caves. Reflection of light on the wall surface of cave might be caused by formation of beads of water caused by hydrophobic filaments of actinobacterial colonies. Main archaeal sequences (ca. 65.7% of total reads) were related with those of I.1a-Associated group of phylum Thaumarchaeota. The sequences were related with that of Candidatus Nitrosotalea devanaterra which was known to oxidize ammonia under acidic condition (ca. pH 5.0). Nutrients leached through volcanic soils contribute formation of unique microbial communities of wall biofilm of cave Daesubee.

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Section: )와 가장 가까운 근연관계가 있음을 확인할 수 있었다(16sunclassified

A Unique Prokaryotic Assemblage of Wall Biofilm of a Volcanic Cave (Daesubee) in Jeju

Moon¹,

Jung²,

Kim³

et al. 2013

The Korean Journal of Microbiology

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“…Compared to MATH, the contact angle method, which consists in estimating the surface energy by measuring the contact angle of the drops deposited on the studied surfaces, requires very elaborate, specifc equipment [18][19][20]. In the case of Streptomyces, the microbial hydrocarbon adhesion method (MATH) was frst used by and more recently by Ding and Lammler [21] and N. Alonso [22] to evaluate the surface hydrophobicity.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Streptomyces Cell Surface by the Microbial Adhesion to Solvents Method

Zanane

Taoufik

2023

International Journal of Microbiology

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The cell surface physicochemical properties of Streptomyces should influencing the dispersal and adsorption of spores and hyphae in soil and should conditioning there interactions with organic or metal substances in the bioremediation of contaminated environment. These properties are concerning surface hydrophobicity, electron donor/acceptor, and charge surface. To date, only hydrophobicity of Streptomyces was studied by contact angle measurements and microbial adhesion to hydrocarbons (MATH). In this work, we studied the electron donor/acceptor character of the Streptomyces cell surface in two ionic strength 10−3 M and 10−1 M of KNO3. Thus, to facilitate the characterisation of the surfaces of microbial cells, we used a simple, rapid, and quantitative technique, the microbial adhesion method to solvents (MATS), which is based on the comparison of the affinity of microbial cells for a monopolar solvent with a polar solvent. The monopolar solvent can be acid (electron acceptor) or basic (electron donor), but both solvents should have a surface tension similar to that of the Kifshitz van der Waals components. At the significant ionic strength of the biological medium, the electron donor character is well expressed for all 14 Streptomyces strains with very significant differences among them ranging from 0% to 72.92%. When the cells were placed in a solution with a higher ionic strength, we were able to classify the donor character results into three categories. The first category is that the weak donor character of strains A53 and A58 became more expressed at 10−1 M KNO3 concentration. The second category is that three strains A30, A60, and A63 expressed a weaker character in a higher ionic strength. For the other strains, no expression of the donor trait was obtained at higher ionic strength. In a suspension with a concentration of 10−3 KNO3, only two strains expressed an electron acceptor character. This character is very important for strains A49, A57, A58, A60, A63, and A65 at 10−1M KNO3. This work has shown that these properties vary greatly depending on the Streptomyces strain. It is important to consider the change in physicochemical properties of surface cells with ionic strength when using Streptomyces in different bioprocesses.

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Adherence of actinomyces pyogenes to HeLa cells mediated by hydrophobic surface proteins

Ding

Lämmler

Seleim

1993

Zentralblatt für Bakteriologie

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Cell Surface Hydrophobicity of Actinomyces pyogenes Determined by Hexadecane Adherence‐ and Salt Aggregation Studies

Cited by 6 publications

References 28 publications

A Unique Prokaryotic Assemblage of Wall Biofilm of a Volcanic Cave (Daesubee) in Jeju

A Unique Prokaryotic Assemblage of Wall Biofilm of a Volcanic Cave (Daesubee) in Jeju

Characterization of Streptomyces Cell Surface by the Microbial Adhesion to Solvents Method

Adherence of actinomyces pyogenes to HeLa cells mediated by hydrophobic surface proteins

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